Ppp2r2b (NM_027531) Mouse Tagged ORF Clone

CAT#: MR207057

Ppp2r2b (Myc-DDK-tagged) - Mouse protein phosphatase 2 (formerly 2A), regulatory subunit B (PR 52), beta isoform (Ppp2r2b), transcript variant 1

Product Images

Circular map for MR207057

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ppp2r2b
• Synonyms: 2900026H06Rik; 6330404L05Rik; E130009M08Rik; PP2A-PR55B; PR55-BETA; SCA12
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207057 representing NM_027531
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGGACATTGATACCCGCAAAATCAACAACAGTTTCCTGCGCGACCACAGCTATGCAACCGAAG
  CTGACATTATCTCTACGGTAGAATTCAACCACACGGGAGAGTTACTAGCGACGGGGGACAAGGGGGGTCG
  GGTCGTAATATTTCAGCGAGAACAGGAGAGTAAAAACCAGGTTCATCGTAGGGGTGAATACAATGTTTAC
  AGCACATTCCAGAGCCATGAACCCGAGTTCGATTACCTGAAAAGTTTAGAAATAGAAGAAAAAATCAATA
  AGATAAGATGGCTCCCTCAACAAAACGCAGCTTACTTTCTTCTGTCTACCAACGATAAAACTGTGAAGCT
  GTGGAAAGTCAGTGAGCGGGATAAGAGGCCAGAAGGCTACAACCTGAAAGATGAGGAGGGCCGGCTCCGG
  GATCCTGCCACCATCACAACCCTGCGGGTGCCTGTCCTGAGACCCATGGACCTGATGGTGGAGGCCACCC
  CACGAAGAGTGTTTGCCAATGCGCACACGTACCACATCAACTCTATATCTGTCAACAGCGACTATGAGAC
  CTACATGTCTGCTGATGACCTGAGGATTAATCTGTGGAACTTTGAGATAACCAATCAGAGTTTTAATATC
  GTGGACATCAAGCCAGCCAACATGGAGGAGCTCACAGAGGTGATCACTGCAGCTGAGTTCCACCCCCACC
  ACTGCAACACCTTCGTATACAGCAGCAGCAAGGGGACAATCCGGCTGTGTGACATGCGTGCATCTGCGCT
  GTGTGACAGGCACACCAAGTTTTTTGAAGAACCGGAAGATCCAAGCAACAGATCGTTTTTCTCTGAAATC
  ATCTCTTCAATTTCGGATGTGAAATTCAGCCACAGTGGGAGGTATATCATGACCAGAGACTATCTGACCG
  TGAAAGTCTGGGACCTCAACATGGAAAATCGCCCCATTGAGACATACCAGGTTCATGACTACCTCCGCAG
  CAAGTTGTGCTCCCTCTATGAAAACGACTGCATTTTTGATAAATTTGAGTGTGTATGGAATGGGTCAGAC
  AGCGTCATCATGACAGGCTCCTACAATAACTTCTTCAGGATGTTCGACCGAAACACCAAGCGTGATGTGA
  CCCTTGAGGCCTCAAGGGAAAACAGCAAACCCCGGGCTATCCTCAAACCCCGAAAAGTGTGCGTCGGGGG
  CAAGCGAAGAAAAGATGAGATCAGTGTTGACAGTCTGGACTTTAGCAAAAAGATCTTGCATACAGCTTGG
  CATCCTTCAGAAAACATTATAGCAGTGGCGGCTACGAATAACTTATATATATTCCAAGACAAGGTTAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207057 representing NM_027531
  Red=Cloning site Green=Tags(s)
  
  MEEDIDTRKINNSFLRDHSYATEADIISTVEFNHTGELLATGDKGGRVVIFQREQESKNQVHRRGEYNVY
  STFQSHEPEFDYLKSLEIEEKINKIRWLPQQNAAYFLLSTNDKTVKLWKVSERDKRPEGYNLKDEEGRLR
  DPATITTLRVPVLRPMDLMVEATPRRVFANAHTYHINSISVNSDYETYMSADDLRINLWNFEITNQSFNI
  VDIKPANMEELTEVITAAEFHPHHCNTFVYSSSKGTIRLCDMRASALCDRHTKFFEEPEDPSNRSFFSEI
  ISSISDVKFSHSGRYIMTRDYLTVKVWDLNMENRPIETYQVHDYLRSKLCSLYENDCIFDKFECVWNGSD
  SVIMTGSYNNFFRMFDRNTKRDVTLEASRENSKPRAILKPRKVCVGGKRRKDEISVDSLDFSKKILHTAW
  HPSENIIAVAATNNLYIFQDKVN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027531
• ORF Size: 1329 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027531.2
• RefSeq Size: 2003 bp
• RefSeq ORF: 1332 bp
• Locus ID: 72930
• Cytogenetics: 18 B3
• MW: 73.4 kDa
• Gene Summary: The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. Within the PP2A holoenzyme complex, isoform 2 is required to promote proapoptotic activity. Isoform 2 regulates neuronal survival through the mitochondrial fission and fusion balance.[UniProtKB/Swiss-Prot Function]