Ntng1 (NM_001163349) Mouse Tagged ORF Clone

CAT#: MR206979

Ntng1 (Myc-DDK-tagged) - Mouse netrin G1 (Ntng1), transcript variant c

Product Images

Circular map for MR206979

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ntng1
• Synonyms: A930010C08Rik; AI853992; Lmnt1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206979 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATTTGTCAAGATTCCTGTCGATCCATGCCCTGTGGGTGACAGTGTCCTCTGTGATGCAGCCCTACC
  TTTTCGTGTGGGGACATTATGATGTATGTAAGAGCCTGATTTACACAGAAGAAGGCAAAGTTTGGGATTA
  CACAGCCTGCCAGCCGGAATCCACGGACATGACCAAGTATCTGAAAGTGAAACTGGACCCTCCGGATATT
  ACCTGTGGAGACCCTCCAGAGTCCTTCTGTGCAATGGGCAACCCTTACATGTGCAATAATGAGTGTGATG
  CGAGTACCCCTGAACTGGCACACCCTCCTGAGCTGATGTTTGATTTTGAAGGAAGACATCCCTCCACATT
  TTGGCAGTCTGCTACTTGGAAGGAGTACCCCAAACCTCTCCAGGTTAACATCACTCTGTCTTGGAGCAAA
  ACCATTGAACTCACAGACAACATAGTTATTACCTTTGAATCGGGGCGTCCAGACCAAATGATCCTAGAGA
  AATCTCTCGACTACGGACGAACATGGCAGCCCTATCAGTATTATGCCACAGACTGCCTCCATGCATTCCA
  CATGGACCCGAAATCCGTGAAGGATTTATCTCAGCACACGGTCTTGGAAATCATTTGCACGGAAGAGTAC
  TCCACTGGGTACTCCACGAATAGCAAAATAATCCACTTCGAGATCAAAGACAGGTTTGCGTTTTTCGCTG
  GACCTCGGCTACGAAATATGGCTTCCCTCTATGGACAGCTGGATACAACCAAGAAACTCAGAGATTTCTT
  CACTGTCACAGACCTGAGGATCAGGCTGTTGAGACCCGCCGTTGGGGAAATATTTGTAGATGAACTACAT
  TTGGCACGTTACTTTTATGCGATCTCAGACATAAAGGTGCGAGGAAGGTGCAAGTGCAACCTGCATGCCA
  CTTCGTGTTTGTATGACAACAGCAAACTGACATGTGAATGTGAGCACAACACTACAGGTCCCGACTGTGG
  GAAATGCAAGAAGAACTACCAGGGCCGACCTTGGAGCCCCGGCTCATACCTCCCCATCCCCAAAGGCACC
  GCAAACACCTGTATCCCCAGCATTTCCAGTATCGGTACTAATGTCTGCGACAATGAGCTCCTGCACTGCC
  AGAATGGAGGGACCTGCCAGAACAATGTGCGCTGCGCGTGCCCAGACGCCTACACCGGCATCCTCTGTGA
  GAAGCTACGGTGCGAAGAGGCGGGCAGCTGTGGCTCCGAATCCGGCCAGGGAGCACCCCCGCGGGGCTCC
  CCAGCACTGCTGCTGCTGACCATGCTGCTGGGGACTGCCGGTCCCCTGGTGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206979 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MYLSRFLSIHALWVTVSSVMQPYLFVWGHYDVCKSLIYTEEGKVWDYTACQPESTDMTKYLKVKLDPPDI
  TCGDPPESFCAMGNPYMCNNECDASTPELAHPPELMFDFEGRHPSTFWQSATWKEYPKPLQVNITLSWSK
  TIELTDNIVITFESGRPDQMILEKSLDYGRTWQPYQYYATDCLHAFHMDPKSVKDLSQHTVLEIICTEEY
  STGYSTNSKIIHFEIKDRFAFFAGPRLRNMASLYGQLDTTKKLRDFFTVTDLRIRLLRPAVGEIFVDELH
  LARYFYAISDIKVRGRCKCNLHATSCLYDNSKLTCECEHNTTGPDCGKCKKNYQGRPWSPGSYLPIPKGT
  ANTCIPSISSIGTNVCDNELLHCQNGGTCQNNVRCACPDAYTGILCEKLRCEEAGSCGSESGQGAPPRGS
  PALLLLTMLLGTAGPLVF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163349
• ORF Size: 1317 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163349.1, NP_001156821.1
• RefSeq Size: 5044 bp
• RefSeq ORF: 1317 bp
• Locus ID: 80883
• UniProt ID: Q8R4G0
• Cytogenetics: 3 F3
• MW: 49.3 kDa
• Gene Summary: Involved in controlling patterning and neuronal circuit formation at the laminar, cellular, subcellular and synaptic levels. Promotes neurite outgrowth of both axons and dendrites.[UniProtKB/Swiss-Prot Function]