Alg1 (BC011281) Mouse Tagged ORF Clone

CAT#: MR206977

Alg1 (Myc-DDK-tagged) - Mouse asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase) (cDNA clone MGC:18946

Product Images

Circular map for MR206977

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Alg1
• Synonyms: HMT1, HMAT1, MGC18946
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206977 representing BC011281
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206977 representing BC011281
  Red=Cloning site Green=Tags(s)
  
  MAASCVALLVLALLLLVLLLGLWKRGRQTGRARHMVVVVLGDVGRSPRMQYHALSLAQSGFSVTLLGFYT
  GPRILQYGVKVVFQAVYLLWKMMRMDPAAYIFLQNPPGLPAIAVCWFVGCICGSKLVIDWHNYGYSIMGL
  VHGPRHPIVLLAKWYEKFFGRLSHLNLCVTNAMREDLAENWCVRAVTLYDKPASFFKETPLDLQHELFMK
  LSHTYSPFQSCSDPSHPDTERSAFTERDCQSGVVRRLHGRPALLVSSTSWTEDEDFSILLRALEKFEQQA
  LTGDSLPSLVCVITGKGPLREHYRHLISQKHLRHIRFCTPWLEAEDYPLLLGSADLGVCLHMSSSGLDLP
  MKVVDMFGCHLPVCAVNFKCLHELVRHGENGLVFKDAEELAAQLQMLFSKFPDPAGKLSQFRKKLQESRQ
  QRWDESWQHTVLPLLTHR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC011281
• ORF Size: 1316 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC011281
• RefSeq Size: 1677 bp
• RefSeq ORF: 1316 bp
• Locus ID: 208211
• Cytogenetics: 16 A1
• MW: 61.5 kDa
• Gene Summary: Participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. Involved in assembling the dolichol-pyrophosphate-GlcNAc(2)-Man(5) intermediate on the cytoplasmic surface of the ER (By similarity).[UniProtKB/Swiss-Prot Function]