Sugct (NM_138654) Mouse Tagged ORF Clone

CAT#: MR206951

5033411D12Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 5033411D12 gene (5033411D12Rik)

Product Images

Circular map for MR206951

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sugct
• Synonyms: 5033411D12Rik; D17907
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206951 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTGGATGCTGGCCAGGGCGGTGGCTTTTCGGAGACCCGGCCGAGGCCTGGCCGGCGGGAGGGGGC
  TGTGGACCGGCCGCCCGCAGTCAGATTGTGACAGTATGAAGCCCCTGGAGGGTGTGAGAATTCTGGATCT
  AACAAGAGTACTGGCAGGACCCTTTGCCACGATGAATTTAGGAGATCTTGGAGCAGAAGTTATAAAAGTT
  GAAAGACCAGGAGCTGGTGATGACACACGATCTTGGGGACCTCCTTTTGTGAACACTGAAAGTACATATT
  TTCTTAGTGTTAATCGAAATAAAAAAAGTATAGCTGTTAATATCAAGGATCCAAGAGGAGTGAGAATCGT
  CAAAGAGCTTGCAGCCATTTGTGATGTGTTTGTGGAAAACTATGTTCCTGGGAAACTTTCTGAGATGGGC
  CTAGGATATGAAGATATAGATAAGATTGCTCCTCACATCATCTACTGCTCCATCACAGGATATGGCCAGA
  CAGGGCCCATGTCTCACAGAGCAGGTTATGATGCTATTGCTTCTGCTATGTCTGGTCTAATGCACATCAC
  AGGACCTGAGGATGGAGATCCTGTTCGCCCTGGAGTGGCCATGACTGACCTCGCCACTGGTCTTTTTGCT
  TATGGGGCCATTATGGCTGGCCTCCTACAACGTTATAGAACTGGAAAAGGACTGTTCATTGATTGTAATC
  TGCTGTCATCACAGGTGGCATGTTTGACCCAAGTAGCTGCTAATTATCTTATTGGACAAAAGGAAGCGAA
  ACGCTGGGGCACAGCTCATGGCAGTATTGTTCCTTATCAGGCTTTTAAAACCAAGGATGGCTATCTTGTA
  ATTGGAGCAGGAAATAACCAACAGTTTGCTGTGGTATGCAAGATCTTGAATTTGCCTGAACTGATCGATG
  ATTCCAAGTATAGAACGAACCATCTTCGGGTACAGAATAGAAAAGAGCTTGTGAAAATCCTTTCAGCACG
  GTTTGCAGAAGAAGTGACTGCCAAGTGGCTTTGTCTCTTTGAAGGAAGTGGGATCCCATATGGACCAATC
  AACAGCCTGAAGGATGTGTTTTCAGAAGCCCAGGTATTGCACAATGGCCTTGTTATGGAGATGAATCATC
  CAACTGTGGGGAAGATTTCAGTCCCAGGTCCAGCTGTGAGATACAGCAAGTTCAAGATGTCAGAGGCAAA
  GCCACCTCCCCTGCTGGGACAGCATACAAGGCATATCCTGAAGGAGGTCCTCAGATATGATGAGGGGGCT
  ATTGAGAAGCTACTCTGCTCTGGTGTGATAGAGCAACATGAAACCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206951 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLWMLARAVAFRRPGRGLAGGRGLWTGRPQSDCDSMKPLEGVRILDLTRVLAGPFATMNLGDLGAEVIKV
  ERPGAGDDTRSWGPPFVNTESTYFLSVNRNKKSIAVNIKDPRGVRIVKELAAICDVFVENYVPGKLSEMG
  LGYEDIDKIAPHIIYCSITGYGQTGPMSHRAGYDAIASAMSGLMHITGPEDGDPVRPGVAMTDLATGLFA
  YGAIMAGLLQRYRTGKGLFIDCNLLSSQVACLTQVAANYLIGQKEAKRWGTAHGSIVPYQAFKTKDGYLV
  IGAGNNQQFAVVCKILNLPELIDDSKYRTNHLRVQNRKELVKILSARFAEEVTAKWLCLFEGSGIPYGPI
  NSLKDVFSEAQVLHNGLVMEMNHPTVGKISVPGPAVRYSKFKMSEAKPPPLLGQHTRHILKEVLRYDEGA
  IEKLLCSGVIEQHETK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138654
• ORF Size: 1311 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138654.2, NP_619595.2
• RefSeq Size: 1727 bp
• RefSeq ORF: 1311 bp
• Locus ID: 192136
• UniProt ID: Q7TNE1
• Cytogenetics: 13 A2
• MW: 47.7 kDa
• Gene Summary: Catalyzes the succinyl-CoA-dependent conversion of glutarate to glutaryl-CoA. Can use different dicarboxylic acids as CoA acceptors, the preferred ones are glutarate, succinate, adipate, and 3-hydroxymethylglutarate (By similarity).[UniProtKB/Swiss-Prot Function]