Asb6 (NM_133346) Mouse Tagged ORF Clone

CAT#: MR206631

Asb6 (Myc-DDK-tagged) - Mouse ankyrin repeat and SOCS box-containing 6 (Asb6)

Product Images

Circular map for MR206631

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Asb6
• Synonyms: 2510004M11Rik; AA409356
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206631 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGTTCTTGCACGGCTTCCGCAGGATCATCTTTGAGTACCAGCCCCTAGTGGACGCCATCCTTGGCG
  CCCTGGGCATCCAGGATCTGGAGCGGCAGGAGCCCCTGGATGATTCTGCTTCCAGCGAGGAGAGCCGGAT
  CCTGGTCCTCACGGAGCTGCTGGAGCAGAAGGCTCACTCTCCATTCTACCAGGAAGGCGTGAGCAATGCG
  TTGCTGAAGATGGCTGAGCTGGGCCTGACCCGGGCAGCTGCTGTCCTTCTGCAGAGTGGGGCCAACCTCA
  ATTTCGAAGACCCTGTTACCTACTACACAGCCCTGCACATTGCTGTCCTGAGAAACCAGCCTGACATGGT
  TGAGCTGCTGGTGCGCCACGGGGCTGACATCAACAGGAGGGACCGGATCCATGAGAGCAGCCCCTTGGAT
  CTGGCCAGCGAGGAACCCGAACGCCTGCCCTGCCTGCAGCGCCTCTTGGATCTTGGAGCAGATGTCAATG
  CAGCTGACAAGAATGGGAAGACAGCTTTACTTCACGCCCTGGCCAGCAGCGATGGTGTGCAGATCCACAA
  CACAGATAACATCCGGCTCCTCCTGGAGGGAGGGGCAGACGTCAAGGCCACCACCAAGGATGGGGACACT
  GTATTCACCTGCATCATCTTCCTACTCGGTGAGACTGTCTGTGGGGACAAGGAGGAGGCCCCGATGATCA
  ACCGCTTCTGCTTCCAAGTCACGCAGCTCTTGCTGGCCCACGGTGCCGACCCCAGCGAGTGCCCGGCCCA
  TGAGTCCCTCACGCACATCTGCCTCAAGAGCTTCAAGCTGCACTTCCCACTCCTCTGCTTCCTGCTGGAG
  TCCGGAGCCGCCTACAACTGCTCCCTGCACGGTGCATCCTGTTGGTCTGGCTTCAACCTCGTTTTTGAGA
  GGCTCTGCTCGCACCCGGGCTGTGCCGAGGACGACAGCCACATTGAGCTTCTGCATAAGGCTGAGACCGT
  GCTGGACCTCATGGTGACCAGCTCCCAGAGGCTGCAGCTGCCTGAGAACCTCAACATCCACCCAGTGGGT
  AGCCTGGCAGGGAAGATCCAGGCCCTTCATGCCTCCCTGAGGCAGCTCGAGAGCTACCCGCCACCTCTCA
  AACACCTGTGCCGGGTGTCCATCCGGCTGTGCCTGCGACCGTGGCCTGTGGACACCAAGGTCAAAGCACT
  GCCCTTGCCCGACAGGCTCAAGTGGTACCTGCTCAGTGCACACAGTGATACCCAAGACACTTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206631 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPFLHGFRRIIFEYQPLVDAILGALGIQDLERQEPLDDSASSEESRILVLTELLEQKAHSPFYQEGVSNA
  LLKMAELGLTRAAAVLLQSGANLNFEDPVTYYTALHIAVLRNQPDMVELLVRHGADINRRDRIHESSPLD
  LASEEPERLPCLQRLLDLGADVNAADKNGKTALLHALASSDGVQIHNTDNIRLLLEGGADVKATTKDGDT
  VFTCIIFLLGETVCGDKEEAPMINRFCFQVTQLLLAHGADPSECPAHESLTHICLKSFKLHFPLLCFLLE
  SGAAYNCSLHGASCWSGFNLVFERLCSHPGCAEDDSHIELLHKAETVLDLMVTSSQRLQLPENLNIHPVG
  SLAGKIQALHASLRQLESYPPPLKHLCRVSIRLCLRPWPVDTKVKALPLPDRLKWYLLSAHSDTQDTC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133346
• ORF Size: 1257 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133346.1, NM_133346.2, NP_579924.1
• RefSeq Size: 2129 bp
• RefSeq ORF: 1257 bp
• Locus ID: 72323
• UniProt ID: Q91ZU1
• Cytogenetics: 2 B
• MW: 46.3 kDa
• Gene Summary: Probable substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.[UniProtKB/Swiss-Prot Function]