Ing3 (NM_023626) Mouse Tagged ORF Clone

CAT#: MR206480

Ing3 (Myc-DDK-tagged) - Mouse inhibitor of growth family, member 3 (Ing3)

Product Images

Circular map for MR206480

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ing3
• Synonyms: 1300013A07Rik; P47ING3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206480 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTGAGCAGCTTCCGATGGACCTGCGGGACCGCTTCACGGAGATGCGCGAGATGGATCTGCAGGTGC
  AGAATGCAATGGATCAACTAGAACAAAGAGTCAGTGAGTTCTTTATGAATGCAAAGAAGAATAAACCCGA
  GTGGCGAGAAGAACAGATGGCATCCATCAAAAAAGACTACTATAAAGCTTTGGAAGATGCAGATGAGAAA
  GTACAGTTGGCAAACCAGATTTATGATTTGGTAGATCGCCACTTGAGAAAGCTGGATCAGGAACTGGCCA
  AGTTTAAAATGGAGCTGGAAGCCGATAATGCTGGAATTACAGAAATACTAGAGAGGCGATCTTTGGAATT
  AGATGCTCCTTCGCAGCCAGTGAACAATCACCACGCTCATTCACATACTCCCGTGGAAAAAAGGAAATAT
  AATCCAACTTCTCACCATGCAGCAGCAGACCATATCCCTGAGAAGAAATTCAAATCTGAAGCGCTGCTGT
  CCACCCTTACATCTGACGCTTCCAAGGAGAACACGCTGGGTTGTCGGAATAATAATTCCACAGCTTCCTG
  CAACAATGCTTACAATGTGAACTCGTCCCAGCCCCTGGCCTCCTATAACATTGGTTCATTATCTTCAGGG
  GCTGGCGCAGGGGCCATCACCATGGCGGCAGCACAGGCCGTGCAAGCGACAGCACAGATGAAAGAAGGGA
  GAAGAACATCGAGTTTAAAAGCCAGTTACGAAGCATTTAAGAACAATGACTTCCAGCTGGGAAAAGAATT
  TTCCATACCCAGAGAAACAGCTGGCTACTCCTCCTCATCGGCACTCATGACAACCTTAACCCAGAATGCC
  AGTTCCTCCGCCACGGACTCGCGGAGTGGCAGGAAGAGCAAAAACAACACCAAGTCTTCAAGCCAGCAGT
  CGTCGTCCTCCTCCTCCTCGTCGTCCTCTTCCTCCTTATCTCTGTGTTCATCGTCTTCAACTGTAGTACA
  AGAAGTTTCCCAGCAAGCAACTGTAGTGCCCGAATCTGACTCCAACAGTCAAGTCGATTGGACTTACGAC
  CCAAATGAACCTCGGTATTGCATCTGTAATCAGGTATCCTATGGAGAGATGGTGGGATGTGATAACCAAG
  ATTGTCCAATAGAATGGTTCCACTATGGGTGTGTTGGCTTGACCGAAGCGCCGAAGGGGAAGTGGTTCTG
  TCCGCAGTGCACCGCTGCCATGAAGAGAAGAGGCAGTCGGCACAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206480 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MIEQLPMDLRDRFTEMREMDLQVQNAMDQLEQRVSEFFMNAKKNKPEWREEQMASIKKDYYKALEDADEK
  VQLANQIYDLVDRHLRKLDQELAKFKMELEADNAGITEILERRSLELDAPSQPVNNHHAHSHTPVEKRKY
  NPTSHHAAADHIPEKKFKSEALLSTLTSDASKENTLGCRNNNSTASCNNAYNVNSSQPLASYNIGSLSSG
  AGAGAITMAAAQAVQATAQMKEGRRTSSLKASYEAFKNNDFQLGKEFSIPRETAGYSSSSALMTTLTQNA
  SSSATDSRSGRKSKNNTKSSSQQSSSSSSSSSSSSLSLCSSSSTVVQEVSQQATVVPESDSNSQVDWTYD
  PNEPRYCICNQVSYGEMVGCDNQDCPIEWFHYGCVGLTEAPKGKWFCPQCTAAMKRRGSRHK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023626
• ORF Size: 1239 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023626.4
• RefSeq Size: 3755 bp
• RefSeq ORF: 1266 bp
• Locus ID: 71777
• UniProt ID: Q8VEK6
• Cytogenetics: 6 A3.1
• MW: 45.7 kDa
• Gene Summary: Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome (By similarity).[UniProtKB/Swiss-Prot Function]