Fam111a (NM_026640) Mouse Tagged ORF Clone

CAT#: MR206440

Fam111a (Myc-DDK-tagged) - Mouse family with sequence similarity 111, member A (Fam111a)

Product Images

Circular map for MR206440

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fam111a
• Synonyms: 4632417K18Rik; AW413625
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206440 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTGTAAGAAGCGGAAATCACAGATCTCATTTAATCCAAGGAAAAATAAAAAAATTAAGGACTATT
  TCTCTCAGGTCCCCAAAGAAGAGCAGAATGACCCCAACACAGTAAAAGTGGACTCTAAAAAAATGCCAAG
  GGATATAACCAACACCAGGGACCAAAGACCACTTTCACCCAGGAAAACTCGACAAGATCAGACTCCTCCC
  CTAAATAAGAAAATCACAGTAACCTTGGGTGTGAACTCTAGGAAACACAAAAACATGAAATATGAGCTCA
  CGTGTAGGGAGACAAGCAGCTTGTATGCAGCACTCAACACTCTCAGTGCTGTCAGAGAAGAGGTAGAAAG
  TCAAAAGGGCAGAGAAATGCTGGTGTGTGGCAAAGAAGGAATTGAAGGGTACTTAAACCTTGGCATGCCC
  GTCTGCTGTATACCAGAAGGCAGCCATGTCGTCATTACATTTTGTCAATGTAAAAGTAAGACACAGGAAA
  ATAAACAATTCTTTGAATCACAGGACCAAGCATCTACCAATTACGTCCGATTTTGCATTCATGCAGTTGG
  GAGTAAGAGGAAAAAGATTCTGAAGTGTGGAGAACTTCAGAAAGAGGGGAACAAACTCTGTGTCTATGGC
  TTCAAAGGAGAGACCATCAGGGACACTCTGAGGAAGGATGGCAGGTTTTGTACTTTCATAGAGAGTGACG
  ATTGGAAACTCATTAATGACCTGGACACCATCATAGAAAACACACAGCCAGTTGATGAGTTAGAGGGCAA
  GCTTTTTCAGGTTGCAGCTGAGCTACCAAAGAACCCCAGAGTAGTCTCTGTCACTCAGAATTCTGGGTCA
  GAGAACAGAAACTTTCATAAGCTAGAAGAGTACATTGTGAATGAGTACACTACATTGAAAGAAGAAGGAA
  AAAAACTTAGAGCATACATCAAGGAAAAAAGTGAAAAAAGGAAGAAGAAAGCTTCCTTATTCAAAGTGCA
  TAAAGAACACTTTGGGAAAATGACAAGAAATTCTACTCCTGTTAAAGTGGTCAAACATCTTTCGAGGGTC
  AGTGACTCAGTTGGGTTCCTATGGTGGAACAACAATGGAAATGCAGGCTGTGCCACCTGCTTTGTTTTTA
  AAGAGTTGTACATTTTGACTTGTCAGCATGTGATAGCTAGCATTGTGGGTGAAGGCATAGATTCAAGTGA
  GTGGGCAAACATAATTAGTCAGTGTGTAAAGGTGACCTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206440 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSCKKRKSQISFNPRKNKKIKDYFSQVPKEEQNDPNTVKVDSKKMPRDITNTRDQRPLSPRKTRQDQTPP
  LNKKITVTLGVNSRKHKNMKYELTCRETSSLYAALNTLSAVREEVESQKGREMLVCGKEGIEGYLNLGMP
  VCCIPEGSHVVITFCQCKSKTQENKQFFESQDQASTNYVRFCIHAVGSKRKKILKCGELQKEGNKLCVYG
  FKGETIRDTLRKDGRFCTFIESDDWKLINDLDTIIENTQPVDELEGKLFQVAAELPKNPRVVSVTQNSGS
  ENRNFHKLEEYIVNEYTTLKEEGKKLRAYIKEKSEKRKKKASLFKVHKEHFGKMTRNSTPVKVVKHLSRV
  SDSVGFLWWNNNGNAGCATCFVFKELYILTCQHVIASIVGEGIDSSEWANIISQCVKVTF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026640
• ORF Size: 1233 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026640.1, NM_026640.2, NP_080916.1
• RefSeq Size: 3199 bp
• RefSeq ORF: 1842 bp
• Locus ID: 107373
• UniProt ID: Q9D2L9
• Cytogenetics: 19
• MW: 46.9 kDa
• Gene Summary: Chromatin-associated protein required for PCNA loading on replication sites. Promotes S-phase entry and DNA synthesis (By similarity).[UniProtKB/Swiss-Prot Function]