Tead2 (BC050217) Mouse Tagged ORF Clone

CAT#: MR206438

Tead2 (Myc-DDK-tagged) - Mouse TEA domain family member 2 (cDNA clone MGC:60551 IMAGE:30021393)

Product Images

Circular map for MR206438

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tead2
• Synonyms: ETF, TEF4, TEAD-2, TEF-4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206438 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGATCCCCGGACTGGGGCCCCCCTGGATGATGGCGGGGGCTGGACAGGTAGCGAGGAAGGCAGCG
  AAGAGGGCACCGGAGGCAGCGAGGGCGTTGGAGGTGATGGCAGCCCTGATGCAGAAGGCCGGAACGAACT
  CATTGCCCGTTACATCAAGCTGAGGACAGGGAAGACGAGAACGCGAAAGCAGGTCTCCAGCCATATTCAG
  GTTTTGGCTCGAAGGAAATCGAGAGAAATTCAGTCCAAGCTGAAGGACCAAGTCTCCAAGGACAAGGCCT
  TCCAGACGATGGCCACCATGTCTTCGGCACAGCTCATCTCCGCCCCTTCCCTCCAGGCCAAGCTGGGCCC
  TTCTGGCCCTCAGGCCACTGAGCTTTTCCAGTTCTGGTCAGGGAGCTCTGGGCCACCATGGAATGTTCCA
  GACGTGAAGCCCTTCTCACAGGCACCGTTCTCCGTGTCACTGACGCCCCCAGCCTCTGACCTACCAGGGT
  ACGAGCCGCCCCCAGCCCTCTCACCCCTGCCCCCACCCGCTCCGTCTCCCCCAGCCTGGCAGGCTCGGGC
  CCTGGGCACTGCCCGCCTGCAGCTGATAGAGTTCTCAGCGTTTGTGGAACCGCCAGACGCAGTTGACTCG
  TTCCAGAGGCATCTGTTTGTCCACATCAGTCAGCAGTGTCCCAGCCCTGGAGCACCACCCCTAGAGAGTG
  TGGACGTGCGGCAGATCTACGACAAATTCCCTGAGAAGAAGGGCGGCCTCCGCGAGCTGTATGACCGAGG
  GCCACCACATGCCTTCTTCCTCGTCAAGTTCTGGGCGGACCTGAACTGGGGCCCCAGTGCCGAGGAGGCA
  GGGAGCAGCGGAGGTGGCGGTGGCTTCTATGGAGTGAGCAGCCAGTATGAGAGCCGGGAGCTCATGACAC
  TCACCTGCTCCTCCAAGGTCTGCTCCTTTGGCAAGCAAGTGGTAGAGAAGGTGGAGACGGAACGGGCCCA
  GCTGGAGGACGGGCGCTTTGTGTACCGTCTGCTGCGCTCTCCCATGTGTGAGTACCTGGTTAATTTCCTG
  CACAAGCTCCGTCAGCTGCCTGAACGCTACATGATGAACAGTGTCCTGGAGAACTTCACCATCCTCCAGG
  TTGTGACAAACAGGGACACTCAGGAACTGCTGCTGTGTACTGCCTACGTCTTTGAAGTCTCCACCAGTGA
  ACGAGGAGCCCAGTACCACATCTACCGCCTGGTCAGGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206438 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGDPRTGAPLDDGGGWTGSEEGSEEGTGGSEGVGGDGSPDAEGRNELIARYIKLRTGKTRTRKQVSSHIQ
  VLARRKSREIQSKLKDQVSKDKAFQTMATMSSAQLISAPSLQAKLGPSGPQATELFQFWSGSSGPPWNVP
  DVKPFSQAPFSVSLTPPASDLPGYEPPPALSPLPPPAPSPPAWQARALGTARLQLIEFSAFVEPPDAVDS
  FQRHLFVHISQQCPSPGAPPLESVDVRQIYDKFPEKKGGLRELYDRGPPHAFFLVKFWADLNWGPSAEEA
  GSSGGGGGFYGVSSQYESRELMTLTCSSKVCSFGKQVVEKVETERAQLEDGRFVYRLLRSPMCEYLVNFL
  HKLRQLPERYMMNSVLENFTILQVVTNRDTQELLLCTAYVFEVSTSERGAQYHIYRLVRD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC050217
• ORF Size: 1230 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC050217, AAH50217
• RefSeq Size: 1951 bp
• RefSeq ORF: 1232 bp
• Locus ID: 21677
• Cytogenetics: 7 29.19 cM
• MW: 45.1 kDa
• Gene Summary: Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction (By similarity). Binds to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif.[UniProtKB/Swiss-Prot Function]