Actr2 (NM_146243) Mouse Tagged ORF Clone

CAT#: MR206161

Actr2 (Myc-DDK-tagged) - Mouse ARP2 actin-related protein 2 homolog (yeast) (Actr2)

Product Images

Circular map for MR206161

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Actr2
• Synonyms: 4921510D23Rik; AA409782; Arp2; D6Ertd746e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206161 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAGCCAGGGCAGGAAGGTGGTGGTGTGCGACAACGGCACTGGGTTTGTGAAGTGTGGATATGCAG
  GCTCTAATTTTCCAGAACACATCTTCCCAGCTTTGGTTGGAAGACCTATTATCAGATCAACCACCAAAGT
  GGGAAACATTGAAATCAAGGACCTTATGGTTGGTGATGAGGCAAGTGAGCTGCGTTCCATGTTGGAAGTG
  AACTACCCTATGGAGAATGGCATCGTGCGGAATTGGGATGACATGAAGCACCTGTGGGACTACACATTTG
  GACCAGAGAAACTCAATATAGACACCAGAAACTGCAAGATTTTACTTACAGAACCTCCCATGAATCCAAC
  CAAAAACAGAGAGAAGATTGTAGAGGTAATGTTTGAAACTTACCAGTTTTCTGGTGTGTATGTAGCCATC
  CAGGCAGTTCTGACTTTGTATGCTCAAGGTTTACTGACTGGTGTAGTAGTGGACTCTGGAGATGGCGTCA
  CTCACATTTGCCCAGTATATGAAGGCTTTTCTCTCCCTCACCTTACAAGGAGGCTGGATATTGCTGGGAG
  GGATATTACCAGGTATCTTATCAAGCTGCTGCTGTTGCGAGGATATGCCTTCAACCATTCTGCTGATTTT
  GAGACAGTTCGCATGATTAAGGAAAAACTTTGTTATGTGGGTTACAATATTGAGCAGGAGCAGAAGCTGG
  CCTTAGAGACCACAGTGTTAGTTGAGTCATATACACTTCCAGATGGACGTATCATTAAGGTTGGAGGAGA
  ACGATTTGAAGCACCAGAGGCTTTATTTCAGCCTCATTTGATCAATGTCGAGGGGGTTGGTGTTGCTGAA
  CTGCTTTTTAACACAATCCAGGCAGCCGACATTGATACCAGATCTGAATTTTACAAGCACATTGTGCTTT
  CTGGAGGTTCTACCATGTATCCTGGCCTGCCATCGAGGTTGGAACGAGAGCTTAAACAGCTTTACTTAGA
  ACGAGTTCTGAAAGGAGATGTGGAAAAACTTTCAAAATTTAAGATCCGAATTGAAGACCCACCCCGCAGG
  AAGCACATGGTGTTCTTGGGTGGCGCAGTCCTAGCAGACATCATGAAAGACAAAGACAACTTTTGGATGA
  CCAGACAAGAGTACCAAGAAAAGGGTGTCCGTGTGCTGGAGAAACTTGGTGTAACTGTTCGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206161 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDSQGRKVVVCDNGTGFVKCGYAGSNFPEHIFPALVGRPIIRSTTKVGNIEIKDLMVGDEASELRSMLEV
  NYPMENGIVRNWDDMKHLWDYTFGPEKLNIDTRNCKILLTEPPMNPTKNREKIVEVMFETYQFSGVYVAI
  QAVLTLYAQGLLTGVVVDSGDGVTHICPVYEGFSLPHLTRRLDIAGRDITRYLIKLLLLRGYAFNHSADF
  ETVRMIKEKLCYVGYNIEQEQKLALETTVLVESYTLPDGRIIKVGGERFEAPEALFQPHLINVEGVGVAE
  LLFNTIQAADIDTRSEFYKHIVLSGGSTMYPGLPSRLERELKQLYLERVLKGDVEKLSKFKIRIEDPPRR
  KHMVFLGGAVLADIMKDKDNFWMTRQEYQEKGVRVLEKLGVTVR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_146243
• ORF Size: 1185 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146243.1, NP_666355.1
• RefSeq Size: 3688 bp
• RefSeq ORF: 1185 bp
• Locus ID: 66713
• UniProt ID: P61161
• Cytogenetics: 11 12.88 cM
• MW: 44.8 kDa
• Gene Summary: ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. Seems to contact the pointed end of the daughter actin filament. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).[UniProtKB/Swiss-Prot Function]