Csnk2a1 (NM_007788) Mouse Tagged ORF Clone

CAT#: MR206103

Csnk2a1 (Myc-DDK-tagged) - Mouse casein kinase 2, alpha 1 polypeptide (Csnk2a1)

Product Images

Circular map for MR206103

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Csnk2a1
• Synonyms: Csnk2a1-rs4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206103 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGGACCCGTGCCAAGCAGGGCCAGAGTTTACACAGATGTTAACACACACAGACCCCGAGAGTACT
  GGGATTATGAATCACATGTGGTGGAATGGGGGAATCAAGATGACTATCAGCTTGTTCGAAAATTAGGTAG
  GGGCAAATACAGTGAAGTGTTTGAAGCCATCAACATCACAAATAATGAAAAAGTTGTTGTTAAAATTCTC
  AAGCCAGTAAAAAAGAAGAAAATTAAGCGTGAAATAAAGATTTTGGAGAATTTGAGAGGTGGGCCCAACA
  TCATCACACTTGCAGACATTGTAAAAGACCCTGTGTCACGAACCCCTGCCTTGGTTTTTGAACATGTAAA
  CAACACAGACTTCAAGCAATTGTACCAGACGTTAACAGACTATGACATTCGATTTTACATGTATGAAATT
  CTGAAAGCCCTGGATTATTGTCACAGCATGGGGATTATGCACAGAGATGTGAAACCCCATAATGTCATGA
  TTGATCATGAGCACAGAAAGCTTCGGCTAATAGACTGGGGTTTAGCTGAGTTTTACCATCCAGGCCAAGA
  GTATAATGTCCGAGTTGCTTCTCGATATTTCAAAGGTCCAGAGCTACTTGTAGATTATCAGATGTACGAT
  TATAGTTTGGATATGTGGAGCTTGGGTTGTATGCTGGCCAGTATGATCTTCCGGAAGGAGCCATTTTTCC
  ATGGACATGACAATTATGATCAGTTGGTGAGGATAGCCAAGGTTCTGGGAACAGAAGATTTATATGACTA
  TATTGACAAGTACAACATTGAATTAGATCCACGTTTCAACGATATCTTGGGCAGACACTCCCGTAAGCGA
  TGGGAACGCTTTGTCCACAGTGAAAACCAGCATCTTGTCAGCCCTGAGGCCTTGGATTTTCTGGACAAGC
  TGCTTCGATATGACCACCAGTCACGGCTCACTGCAAGAGAGGCCATGGAGCACCCTTACTTCTACACTGT
  TGTGAAGGACCAGGCTCGAATGAGTTCCACTAGCATGGCAGGGGGCAGCACACCTGTCAGCAGCGCCAAT
  ATGATGTCAGGGATTTCTTCAGTGCCAACTCCTTCACCCCTTGGACCTCTGGCAGGCTCACCAGTGATTG
  CCGCTGCCAACTCACTTGGGATACCTGTTCCAGCTGCTGCTGGCGCTCAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206103 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSGPVPSRARVYTDVNTHRPREYWDYESHVVEWGNQDDYQLVRKLGRGKYSEVFEAINITNNEKVVVKIL
  KPVKKKKIKREIKILENLRGGPNIITLADIVKDPVSRTPALVFEHVNNTDFKQLYQTLTDYDIRFYMYEI
  LKALDYCHSMGIMHRDVKPHNVMIDHEHRKLRLIDWGLAEFYHPGQEYNVRVASRYFKGPELLVDYQMYD
  YSLDMWSLGCMLASMIFRKEPFFHGHDNYDQLVRIAKVLGTEDLYDYIDKYNIELDPRFNDILGRHSRKR
  WERFVHSENQHLVSPEALDFLDKLLRYDHQSRLTAREAMEHPYFYTVVKDQARMSSTSMAGGSTPVSSAN
  MMSGISSVPTPSPLGPLAGSPVIAAANSLGIPVPAAAGAQQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007788
• ORF Size: 1176 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007788.3, NP_031814.2
• RefSeq Size: 4208 bp
• RefSeq ORF: 1176 bp
• Locus ID: 12995
• UniProt ID: Q60737
• Cytogenetics: 2 G3
• MW: 45.1 kDa
• Gene Summary: Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. It is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythms. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. [provided by RefSeq, Feb 2014]