Cyp2j13 (BC016446) Mouse Tagged ORF Clone

CAT#: MR205809

Cyp2j13 (Myc-DDK-tagged) - Mouse cytochrome P450, family 2, subfamily j, polypeptide 13 (cDNA clone MGC:25927 IMAGE:4235131)

Product Images

Circular map for MR205809

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cyp2j13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205809 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCGAAGCCGCGGACTCCCTGGCAGCTGCCTTCTGGGCAGCTCTCCATCTCCGGACTCTGCTGCTAG
  CTGCTGTCACCTTCCTTTTTCTGGCTGACTACCTCAAAAGCCGGCGCCCCAAGAACTACCCGCCAAGGCC
  GCCGCGCTTGCCCTTTGTGGGCAACTCGTTCCAGATGGACTTGACGCAGTCGCATCTGGCAGTTCAGAAG
  TTTGTGAAGAAGTATGGGAATGTTATCAGTCTTGATTTCGGAATCAAGTCTGCAGTCATTATAAGCAGCC
  TGCCTTTAATCAAAGAAGCCTTTTCTCACCTGGATGAAAACTTTATAAATCGCCGTATATTTCCTCTACA
  AAGACAGATCTTTAATGGAAATGGATTGATCTTCTCCAGTGGCCAGACATGGAAGGAACAAAGGAGGTTT
  GCCCTGATGACACTGAGGAACTTTGGACTGGGAAAGAAGAGCTTAGAGCATCGAATTCAGGAGGAGGCCT
  CCCACCTGGTGGAGGCTATAGGGGAAGAGGAAGGACAGCCTTTTGATCCTCACTTCAAGATCTACAGTGC
  AGTTTCCAATATCATTTGCTCCATCACCTTTGGGGAACGGTTTGAGTACCATGACAGTCAGTTTCAGGAG
  CTGCTGCAGTTACTGGATAAAGCCATGTACTTGGGTACCACCTCCCTAATGGTCCATTACCCAGGCAAGA
  CTACTACAAGTTTCAATGAAGAAAACCTTATCTGCAGCACTCTGGACCTCTTCCTTGCTGGAACAGAGAC
  AACATCCTCAACACTACGATGGGCTCTGCTCTACATGGCCCTATACCCAGAAGTCCAAGAAAATGTACAG
  GCTGAAATTGACCGAGTGATTGGCCAGTCGAAGCATCCCAGCCTGGCTGACAGAGACTCCATGCCCTACA
  CCAATGCTGTTGTCCATGAAATTCTGAGAATGGGCAATAGTGTCCCTCTGAATATTCCCAGGGAAGTGGC
  AGTTGACACTACACTGGCTGGATTTCACTTGCCAAAGGAAAGCGAGCTTGCCTCGGAGAACAATTGGCCA
  GGTTTGAGCTCTTTATTTTTTTCACTGCCCTTGTGCAAAAATTTACCTTCAAGCCCCCAGCCAATGAGAA
  CC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205809 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLEAADSLAAAFWAALHLRTLLLAAVTFLFLADYLKSRRPKNYPPRPPRLPFVGNSFQMDLTQSHLAVQK
  FVKKYGNVISLDFGIKSAVIISSLPLIKEAFSHLDENFINRRIFPLQRQIFNGNGLIFSSGQTWKEQRRF
  ALMTLRNFGLGKKSLEHRIQEEASHLVEAIGEEEGQPFDPHFKIYSAVSNIICSITFGERFEYHDSQFQE
  LLQLLDKAMYLGTTSLMVHYPGKTTTSFNEENLICSTLDLFLAGTETTSSTLRWALLYMALYPEVQENVQ
  AEIDRVIGQSKHPSLADRDSMPYTNAVVHEILRMGNSVPLNIPREVAVDTTLAGFHLPKESELASENNWP
  GLSSLFFSLPLCKNLPSSPQPMRT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC016446
• ORF Size: 1122 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC016446, AAH16446
• RefSeq Size: 1512 bp
• RefSeq ORF: 1124 bp
• Locus ID: 230459
• Cytogenetics: 4 C5
• MW: 42.2 kDa