Exo5 (NM_001160043) Mouse Tagged ORF Clone

CAT#: MR205764

Exo5 (Myc-DDK-tagged) - Mouse defects in morphology 1 homolog (S. cerevisiae) (Dem1), transcript variant 2

Product Images

Circular map for MR205764

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Exo5
• Synonyms: 3110037I16Rik; AV297100; Dem1; Exo V; mExo5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205764 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAGACTGGGGAAGAGGAGACAGCATCAGCAGAAGCCTCAGGGTTTTCAGACTTGAGTGACTCAG
  AGTTAGTTGAATTTCTGGATCTGGAAGAAGCCAAAGAATCGGCTGTTTCACTTAGCAAGCCTGGCCCTTC
  TGCTGAACTCCCTGGGAAGGATGACAAACCAGTAAGCTTGCAGAACTGGAAAGGGGGATTGGATGTCTTG
  TCACCCATGGAGAGATTCCACCTGAAATATTTATATGTCACTGACCTGTGCACTCAGAACTGGTGTGAGT
  TGCAGATGGTGTACGGGAAGGAGCTTCCTGGTTCGTTGACACCTGAGAAAGCAGCTGTTTTGGACACTGG
  TGCTAGCATCCACCTAGCAAAAGAACTAGAACTTCATGACCTTGTGACAGTCCCCATCGCCACAAAAGAA
  GATGCTTGGGCAGTTAAGTTTCTGAACATACTAGCAATGATTCCTGCCCTACAGTCGGAAGGGCGCGTCA
  GAGAGTTTCCAGTGTTTGGGGAGGTGGAGGGAATATTTCTTGTTGGAGTCATTGATGAGTTGCACTACAC
  ATCCAAGGGGGAACTAGAGCTGGCTGAACTCAAGACACGAAGGCGCCCCGTGCTCCCCCTGCCAGCTCAG
  AAAAAGAAAGACTATTTTCAAGTTAGCCTATACAAATATATCTTTGATGCCATGGTACAAGGGAAAGTGA
  CTCCTGCTAGCCTAATCCACCACACTAAATTGTGTCTAGACAAGCCACTGGGACCTTCTGTGCTGAGGCA
  TGCCAGACAAGGAGGCGTGTCTGTAAAATCTTTGGGTGACCTTATGGAACTGGTTTTCTTGTCTCTTACA
  CTGTCTGATCTCCCAGCTATTGATACCCTAAAACTTGAGTATATCCATCAAGAGACTGCCACTATACTGG
  GCACAGAGATTGTAGCCTTTGAAGAGAAGGAAGTGAAAAGCAAGGTGCAGCATTACGTGGCCTACTGGAT
  GGGCCACCGAGATCCTCAAGGCGTTGATGTGGAGGAGGCATGGAAGTGCCGGACCTGTGACTATGTGGAC
  ATCTGCGAGTGGAGGAGGGGCAGTGGAGTGCTCAGCTCATCGTGGGAGCCCAAAGCCAAGAAGTTTAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205764 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAETGEEETASAEASGFSDLSDSELVEFLDLEEAKESAVSLSKPGPSAELPGKDDKPVSLQNWKGGLDVL
  SPMERFHLKYLYVTDLCTQNWCELQMVYGKELPGSLTPEKAAVLDTGASIHLAKELELHDLVTVPIATKE
  DAWAVKFLNILAMIPALQSEGRVREFPVFGEVEGIFLVGVIDELHYTSKGELELAELKTRRRPVLPLPAQ
  KKKDYFQVSLYKYIFDAMVQGKVTPASLIHHTKLCLDKPLGPSVLRHARQGGVSVKSLGDLMELVFLSLT
  LSDLPAIDTLKLEYIHQETATILGTEIVAFEEKEVKSKVQHYVAYWMGHRDPQGVDVEEAWKCRTCDYVD
  ICEWRRGSGVLSSSWEPKAKKFK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001160043
• ORF Size: 1122 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001160043.1, NP_001153515.1
• RefSeq Size: 1969 bp
• RefSeq ORF: 1122 bp
• Locus ID: 73172
• UniProt ID: Q9CXP9
• Cytogenetics: 4 D2.2
• MW: 41.6 kDa
• Gene Summary: Single-stranded DNA (ssDNA) bidirectional exonuclease involved in DNA repair. Probably involved in DNA repair following ultraviolet (UV) irradiation and interstrand cross-links (ICLs) damage. Has both 5'-3' and 3'-5' exonuclease activities with a strong preference for 5'-ends. Acts as a sliding exonuclease that loads at ssDNA ends and then slides along the ssDNA prior to cutting; however the sliding and the 3'-5' exonuclease activities are abolished upon binding to the replication protein A (RPA) complex that enforces 5'-directionality activity (By similarity).[UniProtKB/Swiss-Prot Function]