Macroh2a1 (NM_001159515) Mouse Tagged ORF Clone

CAT#: MR205680

H2afy (Myc-DDK-tagged) - Mouse H2A histone family, member Y (H2afy), transcript variant 4

Product Images

Circular map for MR205680

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Macroh2a1
• Synonyms: H2af; H2AF12; H2AF12M; H2afy; MACROH2; mH2a; mH2a1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205680 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGAGCCGCGGCGGGAAGAAGAAATCCACCAAGACCTCCCGGTCAGCCAAGGCCGGAGTCATCTTCC
  CTGTGGGACGCATGCTTCGGTACATCAAGAAAGGCCACCCTAAGTATAGGATCGGAGTGGGGGCACCTGT
  GTACATGGCTGCTGTCCTGGAGTACCTGACTGCTGAGATCCTGGAGCTGGCTGGCAATGCAGCAAGAGAC
  AACAAGAAGGGACGGGTCACACCCCGGCACATCCTGTTAGCTGTGGCCAATGATGAAGAGCTAAACCAGC
  TGCTAAAGGGTGTCACCATAGCCAGCGGGGGCGTGTTGCCGAATATCCATCCTGAGTTGCTAGCGAAGAA
  GCGAGGATCCAAGGGAAAATTGGAAGCCATCATCACGCCTCCGCCGGCCAAAAAGGCCAAGTCTCCATCC
  CAGAAGAAGCCAGTGGCTAAGAAGACAGGAGGCAAGAAAGGGGCCCGGAAGTCTAAGAAGAAGCAGGGAG
  AAGTGAGCAAGGCGGCCAGCGCAGACAGTACGACGGAGGGCACGCCTACAGACGGCTTCACTGTCCTCTC
  CACCAAGAGCCTCTTCCTCGGCCAGAAGTTGCAAGTTGTTCAGGCTGACATTGCCTCGATCGACAGTGAT
  GCTGTCGTTCACCCGACAAACACTGACTTCTACACCGGTGGTGAAGTAGGAAACACACTGGAGAAGAAGG
  GCGGCAAGGAGTTTGTAGAAGCTGTTCTGGAACTCCGGAAAAAGAACGGGCCCTTGGAGGTAGCTGGAGC
  TGCTATTAGTGCAGGCCATGGCCTGCCTGCCAAGTTTGTGATCCACTGTAATAGTCCTGTCTGGGGTGCA
  GACAAATGTGAAGAACTTCTAGAAAAGACGGTGAAAAACTGCTTGGCTCTAGCTGATGACAGAAAGCTGA
  AATCCATCGCCTTCCCATCCATTGGCAGCGGCAGGAACGGGTTCCCGAAGCAGACAGCGGCCCAGCTCAT
  TCTGAAGGCCATCTCCAGCTACTTTGTCTCCACGATGTCCTCCTCCATCAAAACTGTGTACTTCATGCTT
  TTTGACAGTGAGAGCATAGGTATCTATGTGCAGGAAATGGCCAAGCTGGACGCCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205680 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSRGGKKKSTKTSRSAKAGVIFPVGRMLRYIKKGHPKYRIGVGAPVYMAAVLEYLTAEILELAGNAARD
  NKKGRVTPRHILLAVANDEELNQLLKGVTIASGGVLPNIHPELLAKKRGSKGKLEAIITPPPAKKAKSPS
  QKKPVAKKTGGKKGARKSKKKQGEVSKAASADSTTEGTPTDGFTVLSTKSLFLGQKLQVVQADIASIDSD
  AVVHPTNTDFYTGGEVGNTLEKKGGKEFVEAVLELRKKNGPLEVAGAAISAGHGLPAKFVIHCNSPVWGA
  DKCEELLEKTVKNCLALADDRKLKSIAFPSIGSGRNGFPKQTAAQLILKAISSYFVSTMSSSIKTVYFML
  FDSESIGIYVQEMAKLDAN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001159515
• ORF Size: 1110 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001159515.1, NP_001152987.1
• RefSeq Size: 1966 bp
• RefSeq ORF: 1107 bp
• Locus ID: 26914
• UniProt ID: Q9QZQ8
• Cytogenetics: 13 B1
• MW: 39.3 kDa
• Gene Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2015]