Seh1l (NM_001039088) Mouse Tagged ORF Clone

CAT#: MR205515

Seh1l (Myc-DDK-tagged) - Mouse SEH1-like (S. cerevisiae (Seh1l), transcript variant 1

Product Images

Circular map for MR205515

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Seh1l
• Synonyms: 2610007A16Rik; AW540070; SEC13L; Seh1; SEH1A; SEH1B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205515 representing NM_001039088
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTGTGGCGCGCAGCATCGCGGCGGACCACAAGGACCTCATCCACGATGTGTCTTTCGACTTCCACG
  GGCGCCGGATGGCCACCTGCTCCAGCGATCAGAGCGTCAAGGTGTGGGATAAGAGCGAGAGCGGAGACTG
  GCATTGTACAGCTAGCTGGAAGACACATAGTGGATCTGTATGGCGTGTGACGTGGGCTCATCCTGAATTT
  GGACAAGTTTTGGCTTCCTGTTCTTTTGACCGAACAGCAGCTGTATGGGAAGAAATAGTGGGAGAATCAA
  ACGATAAACTTCGAGGACAGAGTCACTGGGTGAAGAGGACAACTCTAGTGGACAGCAGAACCTCTGTTAC
  TGATGTGAAATTTGCTCCCAAACATATGGGTCTGATGTTGGCCACCTGTTCAGCAGATGGTATAGTAAGG
  GTCTACGAGGCTCCCGATGTGATGAACCTCAGCCAGTGGTCTCTGCAGCACGAGGTCTCGTGTAAGCTGT
  GCTGCAGCTGCATTTCCTGGAACCCTTCCAGCTCTCGTGCTCATCCCCCCATGATCGCTGTGGGAAGTGA
  TGATAGCAGTCCAAATTCAATGGCCAAGGTTCAGATTTTTGAATACAATGAAAATACCAGGAAATATGCG
  AAAGCTGAGACTCTTATGACAGTCACAGACCCCGTCCATGATATCGCGTTTGCTCCAAATTTGGGAAGAT
  CTTTCCACATCCTGGCAGTAGCAACCAAAGATGTAAGAATTTTCACATTAAAACCTCTGAGGAAAGAACT
  TACTTCCTCTGGTGGTCCAACAAAATTTGAAATCCATATTGTGGCTCAGTTTGATAATCATAATTCTCAG
  GTCTGGAGGGTGAGTTGGAACATAACAGGGACAGTCCTGGCGTCTTCAGGAGATGACGGCTGTGTTAGGT
  TGTGGAAAGCTAATTACATGGACAATTGGAAGTGTACTGGTATTTTGAAAGGTAACGGGAGCCCAGTAAA
  TGGGAGTTCTCAGCTGGGAAACTCAAATCCTTCACTGAGCTCAAATATTCCAAATCTTCAAAATTCATTA
  AATGGAACTTCTGCTAGCAGAAAGCACAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205515 representing NM_001039088
  Red=Cloning site Green=Tags(s)
  
  MFVARSIAADHKDLIHDVSFDFHGRRMATCSSDQSVKVWDKSESGDWHCTASWKTHSGSVWRVTWAHPEF
  GQVLASCSFDRTAAVWEEIVGESNDKLRGQSHWVKRTTLVDSRTSVTDVKFAPKHMGLMLATCSADGIVR
  VYEAPDVMNLSQWSLQHEVSCKLCCSCISWNPSSSRAHPPMIAVGSDDSSPNSMAKVQIFEYNENTRKYA
  KAETLMTVTDPVHDIAFAPNLGRSFHILAVATKDVRIFTLKPLRKELTSSGGPTKFEIHIVAQFDNHNSQ
  VWRVSWNITGTVLASSGDDGCVRLWKANYMDNWKCTGILKGNGSPVNGSSQLGNSNPSLSSNIPNLQNSL
  NGTSASRKHS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001039088
• ORF Size: 1080 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039088.1, NP_001034177.1
• RefSeq Size: 3542 bp
• RefSeq ORF: 1083 bp
• Locus ID: 72124
• UniProt ID: Q8R2U0
• Cytogenetics: 18 E1
• MW: 40.2 kDa
• Gene Summary: Component of the Nup107-160 subcomplex of the nuclear pore complex (NPC). The Nup107-160 subcomplex is required for the assembly of a functional NPC. The Nup107-160 subcomplex is also required for normal kinetochore microtubule attachment, mitotic progression and chromosome segregation. This subunit plays a role in recruitment of the Nup107-160 subcomplex to the kinetochore.[UniProtKB/Swiss-Prot Function]