Wdr92 (NM_178909) Mouse Tagged ORF Clone

CAT#: MR205445

Wdr92 (Myc-DDK-tagged) - Mouse WD repeat domain 92 (Wdr92)

Product Images

Circular map for MR205445

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Wdr92
• Synonyms: AI553587; HZGJ
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205445 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCACCTTCGAGAAGCCTCAGATCATTGTCCACATTCAAAAGGGCCTCAACTACACAGTGTTCGACA
  GCAAATGGGTGCCCTGCAGTGCCAAATTCGTGACGATGGGCAACTTTGCCCGGGGCACCGGCGTTATCCA
  GGTGTACGAGATCCAGCGCGGGGACCTCAAGCTGCTGCGGGAGATTGAAAAGGCCAAGCCCATTAAATGT
  GGAACATTTGGTGCCGCATCTCTGCAGCAGAGATTCTTAGCTACTGGAGATTTTGGTGGAAATCTTCATA
  TATGGAACTTGGAAGCTCCAGAGATGCCAGTGTATTCTGTAAAGGGCCACAAAGAAATCATAAATACCAT
  AGATGGTGTAGGTGGACTTGGAATTGGGGAAGGAGCCCCTGAAATCGTGACGGGCAGCCGTGATGGAACT
  GTAAAGGTGTGGGACCCAAGGCAAAAAGAGGATCCTGTCGCTAACATGGAACCTGCACAAGGAGAAAACA
  AGAGAGACTGTTGGACCGTGGCATTTGGCAATGCTTATAATCAAGAAGAACGTGTTGTCTGTGCTGGCTA
  TGACAATGGGGACATCAAGCTGTTTGACCTCAGGAACATGTCACTGCGGTGGGAGACAAACATTAAAAAT
  GGGGTATGCAGCTTGGAATTTGACAGAAAGGACATCAGTATGAATAAGTTGGTAGCTACATCTCTAGAAG
  GGAAGTTCCACGTCTTCGACATGAGAACACAGCATCCTACCAAGGGCTTTGCTTCTGTTACAGAAAAGGC
  CCATAAATCCACTGTATGGCAAGTCCGGCACCTGCCTCAAAACAGGGAGATCTTTCTGACAACGGGAGGC
  GCTGGGAGTCTTCACCTCTGGAAGTATGAATACCCAACTCAGCGGTCAAAGAAAGATTCCGAGGGCTTGG
  AGATGGGAGTCGCAGGTTCCGTCAGCCTTCTGCAGAACGTGACCGTGTCTACTCAGCCCATCTCCAGTTT
  GGACTGGAGTCCTGACAAAAGGGGGCTCTGCGTCTGTAGTTCATTTGACCAGATGGTGCGTGTGCTGATA
  GTTACAAAGCTCCAGACCATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205445 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSTFEKPQIIVHIQKGLNYTVFDSKWVPCSAKFVTMGNFARGTGVIQVYEIQRGDLKLLREIEKAKPIKC
  GTFGAASLQQRFLATGDFGGNLHIWNLEAPEMPVYSVKGHKEIINTIDGVGGLGIGEGAPEIVTGSRDGT
  VKVWDPRQKEDPVANMEPAQGENKRDCWTVAFGNAYNQEERVVCAGYDNGDIKLFDLRNMSLRWETNIKN
  GVCSLEFDRKDISMNKLVATSLEGKFHVFDMRTQHPTKGFASVTEKAHKSTVWQVRHLPQNREIFLTTGG
  AGSLHLWKYEYPTQRSKKDSEGLEMGVAGSVSLLQNVTVSTQPISSLDWSPDKRGLCVCSSFDQMVRVLI
  VTKLQTI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178909
• ORF Size: 1074 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178909.4, NP_849240.1
• RefSeq Size: 3431 bp
• RefSeq ORF: 1074 bp
• Locus ID: 103784
• UniProt ID: Q8BGF3
• Cytogenetics: 11 A2
• MW: 39.8 kDa
• Gene Summary: Seems to act as a modulator of apoptosis.[UniProtKB/Swiss-Prot Function]