Ppp1r8 (NM_146154) Mouse Tagged ORF Clone

CAT#: MR205326

Ppp1r8 (Myc-DDK-tagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 8 (Ppp1r8)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ppp1r8
• Synonyms: 6330548N22Rik; AU044684; NIPP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205326 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCAGCCGTGAACTCCGGCTCCAGCCTCCCGCTGTTCGACTGCCCGACTTGGGCAGGTAAACCAC
  CACCTGGCTTACATCTGGATGTGGTGAAAGGAGACAAGCTAATTGAGAAGCTGATTATTGATGAGAAAAA
  GTATTACTTATTTGGGAGGAACCCCGATCTGTGTGACTTCACTATCGACCACCAGTCATGCTCTCGAGTC
  CACGCAGCGCTGGTGTACCACAAACACCTGAAGAGAGTTTTCTTGATAGACCTCAACAGCACACATGGCA
  CTTTCTTGGGTCACATTCGGCTGGAACCTCACAAGCCTCAACAGATTCCCATCGATTCTACGGTCTCATT
  TGGCGCGTCCACGAGGGCATACACTCTGCGAGAGAAGCCTCAGACATTGCCATCGGCTGTGAAAGGAGAT
  GAGAAGATGGGCGGAGAGGATGATGAACTCAAGGGCTTGTTGGGACTTCCCGAAGAGGAGACCGAGCTGG
  ATAACCTGACAGAGTTCAACACTGCCCACAACAAGCGGATTTCAACCCTCACTATTGAGGAGGGGAACCT
  GGACATCCAGAGACCAAAGAGGAAGAGGAAGAACTCAAGGGTGACCTTCAGTGAGGATGACGAGATCATC
  AACCCAGAGGACGTGGATCCGTCTGTTGGTCGGTTCCGGAACATGGTGCAGACAGCAGTGGTTCCAGTCA
  AGAAGAAGCGGATGGAGGGCTCTGGCTCTCTGGGCCTGGAGGAGTCGGGGAGCAGGCGCATGCAGAACTT
  TGCCTTCAGTGGAGGACTATATGGGGGCCTGCCCCCCACACACAGTGAAACGGGCTCCCAGCCGCATGGC
  ATTCATGGGACAGCGCTCATTGGTGGCTTGCCCATGCCATACCCGAACCTCGCCCCTGATGTGGACTTGA
  CTCCTGTTGTGCCATCAGCAGTGGCCATAAATCCCACACCAAACCCTGCAGTCTATAACCCTGAGGCTGT
  GAATGAACCCAAGAAGAAGAAATACGCGAAGGAGGCTTGGCCGGGCAAGAAGCCCACACCTTCCTTACTG
  ATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205326 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAAVNSGSSLPLFDCPTWAGKPPPGLHLDVVKGDKLIEKLIIDEKKYYLFGRNPDLCDFTIDHQSCSRV
  HAALVYHKHLKRVFLIDLNSTHGTFLGHIRLEPHKPQQIPIDSTVSFGASTRAYTLREKPQTLPSAVKGD
  EKMGGEDDELKGLLGLPEEETELDNLTEFNTAHNKRISTLTIEEGNLDIQRPKRKRKNSRVTFSEDDEII
  NPEDVDPSVGRFRNMVQTAVVPVKKKRMEGSGSLGLEESGSRRMQNFAFSGGLYGGLPPTHSETGSQPHG
  IHGTALIGGLPMPYPNLAPDVDLTPVVPSAVAINPTPNPAVYNPEAVNEPKKKKYAKEAWPGKKPTPSLL
  I
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_146154
• ORF Size: 1056 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146154.3
• RefSeq Size: 2060 bp
• RefSeq ORF: 1056 bp
• Locus ID: 100336
• UniProt ID: Q8R3G1
• Cytogenetics: 4 D2.3
• MW: 38.5 kDa
• Gene Summary: Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Essential for cell proliferation and early embryonic development.[UniProtKB/Swiss-Prot Function]