Faim2 (NM_028224) Mouse Tagged ORF Clone

CAT#: MR204553

Faim2 (Myc-DDK-tagged) - Mouse Fas apoptotic inhibitory molecule 2 (Faim2), transcript variant 1

Product Images

Circular map for MR204553

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Faim2
• Synonyms: 2900002L20Rik; Lfg; Lfg2; NMP25; Nmp35; Tmbim2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204553 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCAGGGAAAGCTCTCTGTGGCTAACAAGGCCCCCGGGACTGAGGGGCAGCAGCACCAGGCAAATG
  GAGAGAAGAAGGATGCTCCGGCAGTGCCCTCGGCGCCACCCTCCTATGAGGAGGCCACCTCTGGAGAGGG
  GCTGAAGGCAGGGACCTTCCCCCAGGGCCCAACAGCTGTGCCACTCCATCCAAGCTGGGCCTACGTGGAC
  CCCAGCGGCAGCTCCGGCTATGAAGGTGGTTTCCCCGCAGGACATCACGAGCATTTCACCACCTTCAGCT
  GGGATGACCAGAAAGTTCGTCGGCTCTTCATCAGAAAGGTCTATACCATCCTATTGGTCCAGCTGCTGGT
  GACCTTGGCTGTGGTGGCTCTCTTTACCTTCTGTGATGTCGTTAAGGACTATGTCCAGGCTAACCCCGGC
  TGGTACTGGGCGTCCTATGCCGTGTTCTTTGCAACTTACCTGACTCTGGCTTGCTGTTCTGGACCCAGGA
  GACATTTCCCTTGGAACCTGATTCTGCTGACCATCTTTACCCTGTCCATGGCCTACCTCACTGGGATGCT
  GTCCAGTTACTACAACACCACATCTGTGCTGCTGTGCCTGGTCATCACGGCTCTCGTCTGCCTCTCGGTC
  ACCATCTTCAGTTTCCAGACCAAGTTTGACTTCACCTCCTGTCAGGGTGTGCTGTTTGTGCTGCTCATGA
  CCCTCTTCTTCAGTGGACTCCTCCTCGCTGTCCTCCTGCCCTTCCAATACGTCCCCTGGCTCCACGCGGT
  CTATGCTGTACTGGGAGCGGGTGTGTTCACGTTGTTCCTGGCCTTTGATACCCAGTTGCTGATGGGTAAC
  CGTCGACACTCGCTGAGCCCCGAGGAGTATATTTTTGGAGCCCTCAACATCTACTTAGACATTATCTATA
  TCTTCACCTTCTTCCTGCAGCTTTTTGGCACCAACCGGGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204553 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTQGKLSVANKAPGTEGQQHQANGEKKDAPAVPSAPPSYEEATSGEGLKAGTFPQGPTAVPLHPSWAYVD
  PSGSSGYEGGFPAGHHEHFTTFSWDDQKVRRLFIRKVYTILLVQLLVTLAVVALFTFCDVVKDYVQANPG
  WYWASYAVFFATYLTLACCSGPRRHFPWNLILLTIFTLSMAYLTGMLSSYYNTTSVLLCLVITALVCLSV
  TIFSFQTKFDFTSCQGVLFVLLMTLFFSGLLLAVLLPFQYVPWLHAVYAVLGAGVFTLFLAFDTQLLMGN
  RRHSLSPEEYIFGALNIYLDIIYIFTFFLQLFGTNRE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028224
• ORF Size: 954 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028224.4, NP_082500.2
• RefSeq Size: 4668 bp
• RefSeq ORF: 954 bp
• Locus ID: 72393
• UniProt ID: Q8K097
• Cytogenetics: 15 F1
• MW: 35.3 kDa
• Gene Summary: Antiapoptotic protein which protects cells uniquely from Fas-induced apoptosis. Regulates Fas-mediated apoptosis in neurons by interfering with caspase-8 activation. Plays a role in cerebellar development by affecting cerebellar size, internal granular layer (IGL) thickness, and Purkinje cell (PC) development.[UniProtKB/Swiss-Prot Function]