Epsti1 (NM_029495) Mouse Tagged ORF Clone

CAT#: MR204486

Epsti1 (Myc-DDK-tagged) - Mouse epithelial stromal interaction 1 (breast) (Epsti1), transcript variant a

Product Images

Circular map for MR204486

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Epsti1
• Synonyms: 2310046K10Rik; 5033415K03Rik; BRESI1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204486 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACACCCGCAGTAAGGTGGTGGGCCCAGGCTTGGGCACTTCCTCCATCTCTCGGGACCACGCTGGAG
  CTGGGCAGCGCAGGGAGCTGGGCTTGCAGCAAAACCGGAGACAGAGTTTGGAGGTGGCAGCTCCCGAGGG
  CCCCAAGATGGAGAGGCAGGGGCATGCTGACCAGGGAAGCGCAGGCACATACACCCTGATAGCACCAAAC
  GAGAGCAGAAGGCAGAAGATACAGAGAATTGCTGAGCAAGAACTGGCAGACCTGGAGAGATGGAAGCAAC
  AGAACAGGGCAAAACCAGTCTACTTGGTGCCACAACGGCTGGGTGGAAGCCAGTCTGAGGCTGAAGTCAG
  ACAGAAACAACAGCTGCAACAGATGCGTTCTAAATACCAGCAAAAGCTCAAGAGGGATGAATCTATTCGA
  ATCAGGAAGGAAGCTGAGGAAGCCAAGTTCCAAAAAATGAAAGCAATCCAGAGGGAGAAGAGTAATAAAC
  TAGAGGAGAAAAAGCAACTTCAAGAAGACATCAGAAGAGCCACATTTCGAGAGCATCATCAGTCCAAAAC
  GGCTGAGCTCTTGAGCAGACTGGACACAGAGCGGAGAAACAGAAGTGCCTGTCTGATTGCTCCTCCTGCC
  ACCCAGTCCTCGAGATGGAAACTTCCAGTCCTGCTTAGAGATCCCAGCTGGGCCGGCAGCCAAGCACACA
  GGGATTCTCCGCAGAAGGAAGACAACCCCAGGCTGCAAAAGACGAGGGATGGACATCAGAAGAATAAATT
  ACTGGAAACTAAAGGACAGCACCAGGAGGAAGAAAGAGCCCAAATCCATCAAGCTGAACACTGGAGGGTA
  AATAATGCTTTTCTGGACCGACTCCAAGGCAAAAGTCAACCAGGTGGCCTCGAGCAGTCTGGAGGCTGTT
  GCAATATGAATAGTACTGACAGCTGGGGTATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204486 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MYTRSKVVGPGLGTSSISRDHAGAGQRRELGLQQNRRQSLEVAAPEGPKMERQGHADQGSAGTYTLIAPN
  ESRRQKIQRIAEQELADLERWKQQNRAKPVYLVPQRLGGSQSEAEVRQKQQLQQMRSKYQQKLKRDESIR
  IRKEAEEAKFQKMKAIQREKSNKLEEKKQLQEDIRRATFREHHQSKTAELLSRLDTERRNRSACLIAPPA
  TQSSRWKLPVLLRDPSWAGSQAHRDSPQKEDNPRLQKTRDGHQKNKLLETKGQHQEEERAQIHQAEHWRV
  NNAFLDRLQGKSQPGGLEQSGGCCNMNSTDSWGI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029495
• ORF Size: 945 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029495.2
• RefSeq Size: 1869 bp
• RefSeq ORF: 945 bp
• Locus ID: 108670
• UniProt ID: Q8VDI1
• Cytogenetics: 14 D3
• MW: 36.1 kDa
• Gene Summary: Plays a role in M1 macrophage polarization and is required for the proper regulation of gene expression during M1 versus M2 macrophage differentiation (PubMed:29217193). Might play a role in RELA/p65 and STAT1 phosphorylation and nuclear localization upon activation of macrophages (PubMed:29217193).[UniProtKB/Swiss-Prot Function]