Crym (NM_016669) Mouse Tagged ORF Clone

CAT#: MR204445

Crym (Myc-DDK-tagged) - Mouse crystallin, mu (Crym)

Product Images

Circular map for MR204445

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Crym
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204445 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCGGGCGCCAGCGTTCCTGAGCGCAGAGGAGGTGCAGGATCACCTTCGCAGCTCCAGCCTTCTCA
  TCCCACCCCTGGAGGCCGCACTGGCCAACTTCTCCAAAGGTCCCGACGGAGGGGTCATGCAGCCAGTGCG
  CACCGTGGTGCCTGTAGCCAAGCACCGAGGCTTCCTGGGAGTCATGCCTGCCTACAGTGCTGCTGAGGAT
  GCGCTCACCACCAAGTTAGTCACCTTCTATGAGGGCCACAGCAACACAGCGGTCCCCTCCCATCAGGCAT
  CGGTGCTTCTCTTTGATCCCAGCAATGGCTCCCTGCTGGCGGTCATGGATGGAAATGTCATAACTGCAAA
  GAGAACAGCAGCGGTGTCTGCCATTGCCACAAAGCTGTTGAAGCCCCCAGGCAGTGATGTGCTGTGCATC
  CTTGGAGCGGGGGTCCAGGCGTACAGTCACTATGAGATCTTCACAGAGCAGTTCTCCTTCAAGGAGGTGA
  GAATGTGGAACCGCACCAGGGAAAATGCTGAGAAGTTTGCAAGCACAGTGCAAGGAGATGTTCGGGTCTG
  TTCATCAGTGCAGGAGGCTGTGACAGGTGCTGATGTCATCATCACAGTCACCATGGCAACAGAGCCCATT
  TTATTTGGTGAATGGGTAAAGCCGGGGGCTCACATCAATGCTGTTGGAGCCAGCAGGCCTGACTGGCGAG
  AACTGGATGACGAGCTCATGAGGCAAGCGGTGCTGTATGTGGACTCCCGGGAGGCTGCCCTGAAGGAGTC
  AGGAGACGTTCTGTTGTCAGGGGCTGACATCTTTGCTGAGCTTGGAGAAGTGATTTCAGGAGCGAAGCCT
  GCACACTGTGAGAAGACCACAGTGTTCAAATCTTTGGGGATGGCAGTGGAAGACCTGGTTGCAGCCAAAT
  TAGTATATGATTCTTGGTCATCTGGCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204445 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKRAPAFLSAEEVQDHLRSSSLLIPPLEAALANFSKGPDGGVMQPVRTVVPVAKHRGFLGVMPAYSAAED
  ALTTKLVTFYEGHSNTAVPSHQASVLLFDPSNGSLLAVMDGNVITAKRTAAVSAIATKLLKPPGSDVLCI
  LGAGVQAYSHYEIFTEQFSFKEVRMWNRTRENAEKFASTVQGDVRVCSSVQEAVTGADVIITVTMATEPI
  LFGEWVKPGAHINAVGASRPDWRELDDELMRQAVLYVDSREAALKESGDVLLSGADIFAELGEVISGAKP
  AHCEKTTVFKSLGMAVEDLVAAKLVYDSWSSGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016669
• ORF Size: 942 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016669.2
• RefSeq Size: 1256 bp
• RefSeq ORF: 942 bp
• Locus ID: 12971
• UniProt ID: O54983
• Cytogenetics: 7 64.47 cM
• MW: 33.5 kDa
• Gene Summary: Specifically catalyzes the reduction of imine bonds in brain substrates that may include cystathionine ketimine (CysK) and lanthionine ketimine (LK). Binds thyroid hormone which is a strong reversible inhibitor. Presumably involved in the regulation of the free intracellular concentration of triiodothyronine and access to its nuclear receptors (By similarity).[UniProtKB/Swiss-Prot Function]