Ift46 (NM_023831) Mouse Tagged ORF Clone
CAT#: MR204208
- TrueORF®
Ift46 (Myc-DDK-tagged) - Mouse intraflagellar transport 46 homolog (Chlamydomonas) (Ift46)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_023831" in other vectors (4)
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USD 198.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Ift46 |
Synonyms | 1500035H01Rik; AA408110; AI663846; c11orf2; R75047 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR204208 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGATAACAGCAGTGACGAGTACGAGGAGGACAATAAGGAGAAGAAGAAGCCCTCCCAGCTGACAC CACAGCAGGGCTTCAGTGAGAATGATGATGACGACGACGATGACTCCTCTGAGACGGATTCCGATGACGA CGATGACGATGAGGAGCATGGAGCACCTCTGGAAGGGGCGTATGATCCTGCCGACTACGAGCATCTGCCA GTCTCGGCCGAGATCAAGGAGCTCTTTGAGTACATCAGCCGGTACACACCTCAGTTGATCGATCTGGACC ACAAACTGAAACCTTTCATTCCTGATTTTATCCCAGCTGTTGGGGATATCGATGCATTTTTAAAGGTGCC ACGTCCCGATGGAAAGCCTGACCACCTTGGCCTCTTGGTGTTAGATGAACCATCGACAAAGCAGTCGGAC CCTACCGTGCTTTCGCTGTGGTTAACAGAGAATTCCAAGCAGCATAATATTACGCAACATATGAAAGTAA AGAGCCTGGAGGATGCAGAGAAGAATCCCAAAGCCATTGACACCTGGATTGAGAGCATCTCTGAGTTACA CCGTTCCAAGCCCCCTGCAACTGTGCACTACACCAGGCCCATGCCCGACATTGACACTCTGATGCAGGAG TGGTCCCCGGAATTTGAAGAGCTCCTGGGAAAGGTGAGTCTGCCCACTGTCGAGATCGATTGCAGCCTGG CTGAGTACATTGACATGATCTGTGCGATCCTGGACATTCCTTTCTATAAGAGCCGGATTCAGTCTCTCCA CCTGCTCTTTTCCCTCTACTCAGAGTTCAAGAACTCCCAGCATTTTAAAGCTCTTGCTGAAGGCAAAAAA GTATTCACCCCTCCACCCAACTCTGCCTCCCAGGCTGGGGATGCGGAGACACTGACTTTCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204208 protein sequence
Red=Cloning site Green=Tags(s) MADNSSDEYEEDNKEKKKPSQLTPQQGFSENDDDDDDDSSETDSDDDDDDEEHGAPLEGAYDPADYEHLP VSAEIKELFEYISRYTPQLIDLDHKLKPFIPDFIPAVGDIDAFLKVPRPDGKPDHLGLLVLDEPSTKQSD PTVLSLWLTENSKQHNITQHMKVKSLEDAEKNPKAIDTWIESISELHRSKPPATVHYTRPMPDIDTLMQE WSPEFEELLGKVSLPTVEIDCSLAEYIDMICAILDIPFYKSRIQSLHLLFSLYSEFKNSQHFKALAEGKK VFTPPPNSASQAGDAETLTFI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_023831 |
ORF Size | 906 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_023831.1, NM_023831.2, NM_023831.3, NP_076320.2 |
RefSeq Size | 3337 bp |
RefSeq ORF | 906 bp |
Locus ID | 76568 |
UniProt ID | Q9DB07 |
Cytogenetics | 9 A5.2 |
MW | 34.1 kDa |
Gene Summary | Forms part of a complex involved in intraflagellar transport (IFT), the bi-directional movement of particles required for the assembly, maintenance and functioning of primary cilia. May play a role in chondrocyte maturation and skeletogenesis.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC205580 | Ift46 (untagged) - Mouse intraflagellar transport 46 homolog (Chlamydomonas) (Ift46), (10ug) |
USD 300.00 |
|
MG204208 | Ift46 (tGFP-tagged) - Mouse RIKEN cDNA 1500035H01 gene (1500035H01Rik) |
USD 500.00 |
|
MR204208L3 | Lenti ORF clone of Ift46 (Myc-DDK-tagged) - Mouse intraflagellar transport 46 homolog (Chlamydomonas) (Ift46) |
USD 600.00 |
|
MR204208L4 | Lenti ORF clone of Ift46 (mGFP-tagged) - Mouse intraflagellar transport 46 homolog (Chlamydomonas) (Ift46) |
USD 600.00 |
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