Mrpl19 (NM_026490) Mouse Tagged ORF Clone

CAT#: MR204003

Mrpl19 (Myc-DDK-tagged) - Mouse mitochondrial ribosomal protein L19 (Mrpl19), nuclear gene encoding mitochondrial protein

Product Images

Circular map for MR204003

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mrpl19
• Synonyms: 9030416F12Rik; 9130412E02Rik; D6Ertd157e; MRP-L15; RLX1; Rpml15
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204003 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCTCCATGGCAGAAAGTTGTAGGGCGTCCCTGTACCTGGCCCGGAGCGTCCGAATGGCCAGGC
  CACGGCTTGCTGCCTTCGCATCTGATGCCTGCCGTGTCTGCACGGGCCCAAGCCGATTTCAGAGCACCGG
  GCCTTCAGAGCCCGGTGGGTTCAAGCCACCGCCGAAACCTGTCATCGTGGACCGGCGCCGAGTCCCGGAA
  GACGAGAGAAGGTTCCTGAGTCCTGAATTCATCCCTCCCAGGGGGAGAACAAACCCTCTGAAATTTAAGA
  TAGAAAGAAAAGATATGCTAGACAGGAGAAAGGTGCTTCCGATTCCAGAGTTCTATGTTGGAAGCATACT
  TCGAGTCACCACAGCCGACCCCTATGCCAGTGGAAAAACCAGCCAGTTCCTGGGGATTTGCATCAAGCGA
  TCAGGGAATGGGCTCGGGGCTACTTTCACCCTCAGGAACACGATTGAAGGACAAGGTGTGGAGATTTGCT
  TTGAACTGTATAATCCTCGAATCCAGGAGATCCAGGTGGTGAAGTTGGAGAAGCGGCTGGATGACAACCT
  GCTGTACTTACGGGACGCCCTTCCCGAGTACAGCACCTTTGACGTGAACATGAAGCCGGTCCCACAGGAG
  GCCTGCCAGGAAGTGCCTGTTAACAAGCTGAAAGTTAAAATGAAGCCAAAGCCGTGGTCCAAACGCTGGG
  AACGTCCAAACTTCAACATTAAGGGAATCAGATTTGACCTTGCTTTAACTGAAGAGCAAATGAAGGAAGC
  CCAGAAGTGGAACAAGCCATGGATTGAGTTTGATATGATGAGAGAATATGACACTTCAAAAATTGAAGCT
  GCATTGTGGGAAGAAATAGAAGCATCGAAGAAGTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204003 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAASMAESCRASLYLARSVRMARPRLAAFASDACRVCTGPSRFQSTGPSEPGGFKPPPKPVIVDRRRVPE
  DERRFLSPEFIPPRGRTNPLKFKIERKDMLDRRKVLPIPEFYVGSILRVTTADPYASGKTSQFLGICIKR
  SGNGLGATFTLRNTIEGQGVEICFELYNPRIQEIQVVKLEKRLDDNLLYLRDALPEYSTFDVNMKPVPQE
  ACQEVPVNKLKVKMKPKPWSKRWERPNFNIKGIRFDLALTEEQMKEAQKWNKPWIEFDMMREYDTSKIEA
  ALWEEIEASKKS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026490
• ORF Size: 879 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026490.2, NP_080766.1
• RefSeq Size: 5016 bp
• RefSeq ORF: 879 bp
• Locus ID: 56284
• UniProt ID: Q9D338
• Cytogenetics: 6 35.81 cM
• MW: 33.6 kDa