Cnot8 (NM_026949) Mouse Tagged ORF Clone

CAT#: MR203998

Cnot8 (Myc-DDK-tagged) - Mouse CCR4-NOT transcription complex, subunit 8 (Cnot8)

Product Images

Circular map for MR203998

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cnot8
• Synonyms: 1500015I04Rik; 1810022F04Rik; AA536816; AU015770; AU043059
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203998 representing NM_026949
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGCGGCACTTGTAGAAAACAGTCAGGTCATCTGTGAGGTGTGGGCCAGCAATCTTGAGGAAGAGA
  TGCGGAAGATCCGTGAGATTGTCCTCAGCTACAGCTATATCGCTATGGACACAGAGTTCCCAGGTGTTGT
  TGTACGGCCGATCGGTGAATTTCGAAGCTCCATAGATTACCAGTATCAGCTTCTGCGGTGCAATGTTGAT
  CTTCTTAAAATCATCCAGCTGGGCCTTACGTTCACCAATGAGAAGGGGGAGTACCCGTCTGGAATCAACA
  CATGGCAGTTCAACTTCAAGTTCAACCTGACAGAGGATATGTACTCCCAGGATTCCATAGATCTGCTTGC
  AAACTCAGGGCTGCAGTTCCAGAAACACGAGGAGGAAGGGATCGATACACTGCACTTTGCAGAGCTGCTT
  ATGACATCGGGAGTGGTTCTCTGTGACAACGTCAAGTGGCTTTCATTTCACAGTGGTTATGATTTTGGCT
  ACATGGTAAAGCTGCTAACAGACTCTCGCCTGCCGGAAGAAGAACACGAGTTCTTTCATATCCTGAATCT
  TTTCTTCCCGTCCATTTACGATGTGAAATACCTGATGAAGAGCTGCAAAAACCTGAAGGGAGGCCTTCAG
  GAGGTGGCTGATCAGTTGGATCTGCAGAGGATCGGAAGGCAGCATCAAGCAGGCTCAGACTCTCTGCTGA
  CGGGGATGGCTTTCTTCAGGATGAAAGAGCTATTCTTTGAGGACAGTATTGACGATGCCAAGTATTGTGG
  GCGCCTCTATGGCCTGGGCACAGGGGTGGCCCAGAAGCAGAATGAGGATGTGGACTGTGCCCAGGAGAAG
  ATGAGCATCTTGGCCATGATCAACAACATGCAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203998 representing NM_026949
  Red=Cloning site Green=Tags(s)
  
  MPAALVENSQVICEVWASNLEEEMRKIREIVLSYSYIAMDTEFPGVVVRPIGEFRSSIDYQYQLLRCNVD
  LLKIIQLGLTFTNEKGEYPSGINTWQFNFKFNLTEDMYSQDSIDLLANSGLQFQKHEEEGIDTLHFAELL
  MTSGVVLCDNVKWLSFHSGYDFGYMVKLLTDSRLPEEEHEFFHILNLFFPSIYDVKYLMKSCKNLKGGLQ
  EVADQLDLQRIGRQHQAGSDSLLTGMAFFRMKELFFEDSIDDAKYCGRLYGLGTGVAQKQNEDVDCAQEK
  MSILAMINNMQQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026949
• ORF Size: 876 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026949.5
• RefSeq Size: 2035 bp
• RefSeq ORF: 879 bp
• Locus ID: 69125
• UniProt ID: Q9D8X5
• Cytogenetics: 11 B1.3
• MW: 33.6 kDa
• Gene Summary: Has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Its function seems to be partially redundant with that of CNOT7. Catalytic component of the CCR4-NOT complex which is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. During miRNA-mediated repression the complex seems also to act as translational repressor during translational initiation. Additional complex functions may be a consequence of its influence on mRNA expression. Associates with members of the BTG family such as TOB1 and BTG2 and is required for their anti-proliferative activity.[UniProtKB/Swiss-Prot Function]