Emc3 (NM_175101) Mouse Tagged ORF Clone

CAT#: MR203415

Emc3 (Myc-DDK-tagged) - Mouse transmembrane protein 111 (Tmem111)

Product Images

Circular map for MR203415

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Emc3
• Synonyms: 0610039A15Rik; AI225901; AW260416; Pob; Tmem111
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203415 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGGCCCGAGCTGCTGCTTGACTCCAACATCCGCCTCTGGGTGGTCCTGCCCATCGTTATCATCA
  CTTTCTTCGTGGGCATGATCCGCCACTACGTGTCAATCCTACTGCAGAGCGACAAGAAGCTCACCCAGGA
  ACAAGTGTCTGACAGTCAGGTCCTAATTCGAAGCAGAGTCCTCAGGGAAAATGGAAAATACATTCCCAAG
  CAGTCTTTCTTAACACGAAAATATTACTTCAACAACCCAGAGGATGGATTTTTCAAAAAAACAAAAAGGA
  AGGTTGTGCCACCTTCCCCCATGACAGACCCCACCATGCTCACAGACATGATGAAAGGGAATGTCACAAA
  TGTCCTCCCAATGATTCTTATCGGCGGATGGATCAACATGACGTTTTCAGGCTTTGTCACAACTAAGGTC
  CCGTTTCCACTGACACTTCGCTTCAAGCCTATGCTTCAGCAAGGAATAGAGCTGCTCACACTAGACGCAT
  CCTGGGTGAGTTCTGCATCCTGGTACTTCCTCAATGTGTTTGGGCTCCGGAGCATTTACTCTCTAATCCT
  GGGCCAAGATAACGCCGCCGACCAGTCACGAATGATGCAGGAGCAGATGACAGGAGCAGCGATGGCCATG
  CCTGCAGACACCAACAAAGCTTTCAAGACAGAGTGGGAAGCTTTGGAACTGACAGATCACCAGTGGGCGC
  TCGATGATGTGGAAGAAGAACTCATGGCCAGAGACCTCCACTTTGAAGGCATGTTCAAAAAGGAACTACA
  GACGTCCATATTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203415 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGPELLLDSNIRLWVVLPIVIITFFVGMIRHYVSILLQSDKKLTQEQVSDSQVLIRSRVLRENGKYIPK
  QSFLTRKYYFNNPEDGFFKKTKRKVVPPSPMTDPTMLTDMMKGNVTNVLPMILIGGWINMTFSGFVTTKV
  PFPLTLRFKPMLQQGIELLTLDASWVSSASWYFLNVFGLRSIYSLILGQDNAADQSRMMQEQMTGAAMAM
  PADTNKAFKTEWEALELTDHQWALDDVEEELMARDLHFEGMFKKELQTSIF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175101
• ORF Size: 786 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175101.3, NP_780310.1
• RefSeq Size: 1958 bp
• RefSeq ORF: 786 bp
• Locus ID: 66087
• UniProt ID: Q99KI3
• Cytogenetics: 6 E3
• MW: 30 kDa
• Gene Summary: Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins. Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues. Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices. It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes. By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors. By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes.[UniProtKB/Swiss-Prot Function]