Barx2 (NM_013800) Mouse Tagged ORF Clone

CAT#: MR203352

Barx2 (Myc-DDK-tagged) - Mouse BarH-like homeobox 2 (Barx2)

Product Images

Circular map for MR203352

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Barx2
• Synonyms: 2310006E12Rik; Barx2b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203352 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCGATGAGATCCTCTCCAAGGAGACCTGCGACTACTTTGAGAAACTTTCCCTCTACTCTGTGTGCC
  CGTCGCTGGTGGTGCGACCCAAGCCCCTGCACTCTTGTACCGGTTCCCCTTCCCTACGGGCATATCCTCT
  CCTGTCTGTGATCACCCGGCAGCCCACAGTCATCTCCCACCTGGTTCCCACCGGCTCGGGACTCACCCCA
  GTGTTAACTCGCCATCCAGTCGCCGCCTCGGAGGCGGCGGCTGCTGCTGCTGCTGAGACCCCTGGTGGTG
  AGGCGTTAGCCAGCAGCGAGTCAGAGACAGAACAGCCCACGCCCAGGCAGAAGAAACCACGCAGAAGTCG
  CACCATCTTCACCGAGCTGCAGCTCATGGGCCTAGAGAAGAAATTCCAGAAGCAGAAGTATTTGTCTACC
  CCAGACAGGTTGGACTTGGCCCAGTCTCTGGGACTCACTCAGCTGCAAGTGAAGACTTGGTATCAGAATC
  GCAGGATGAAATGGAAGAAGATGGTCCTTAAAGGTGGACAGGAAGCACCCACAAAACCTAAGGGGCGCCC
  TAAGAAGAACTCCATTCCCACATCAGAGGAGATTGAAGCTGAAGAGAAGATGAACAGCCAGGCTCAGAGC
  CAGGAGCTGCTGGAATCCTCGGAGAGACAGGAGGAGCCCTGTGATACCCAGGAGCCCAAAGCGTGCCTTG
  TCCCCTTGGAGGTGGCAGAACCTATTCACCAGCCCCAGGAGTTATCAGAAGCTTCCTCTGAACCCCCACC
  ATTAAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203352 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MIDEILSKETCDYFEKLSLYSVCPSLVVRPKPLHSCTGSPSLRAYPLLSVITRQPTVISHLVPTGSGLTP
  VLTRHPVAASEAAAAAAAETPGGEALASSESETEQPTPRQKKPRRSRTIFTELQLMGLEKKFQKQKYLST
  PDRLDLAQSLGLTQLQVKTWYQNRRMKWKKMVLKGGQEAPTKPKGRPKKNSIPTSEEIEAEEKMNSQAQS
  QELLESSERQEEPCDTQEPKACLVPLEVAEPIHQPQELSEASSEPPPLS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013800
• ORF Size: 780 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013800.2, NP_038828.2
• RefSeq Size: 1791 bp
• RefSeq ORF: 852 bp
• Locus ID: 12023
• UniProt ID: O08686
• Cytogenetics: 9 A4
• MW: 28.7 kDa
• Gene Summary: Transcription factor. Binds optimally to the DNA consensus sequence 5'-YYTAATGRTTTTY-3'. May control the expression of neural adhesion molecules such as L1 or Ng-CAM during embryonic development of both the central and peripherical nervous system. May be involved in controlling adhesive processes in keratinizing epithelia.[UniProtKB/Swiss-Prot Function]