Kcnmb2 (NM_028231) Mouse Tagged ORF Clone

CAT#: MR202855

Kcnmb2 (Myc-DDK-tagged) - Mouse potassium large conductance calcium-activated channel, subfamily M, beta member 2 (Kcnmb2)

Product Images

Circular map for MR202855

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kcnmb2
• Synonyms: 2700049B16Rik; 3110031N04Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR202855 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTATATGGACCAGTGGCCGGACCTCTTCATCTTACAGACAGGACGAGAAAAGAAATATCTACCAGA
  AAATCAGGGACCATGACCTCCTGGACAAAAGGAAAACTGTGACAGCTCTGAAGGCTGGGGAGGACCGGGC
  CATCCTGCTCGGCCTGGCCATGATGGTGTGCTCCATCATGATGTACTTCCTGCTGGGAATCACACTGCTG
  CGCTCCTACATGCAGAGCGTGTGGACAGAAGAAGCCCAGTGTGCCCTGCTGAATGTGTCAATCACAGAAA
  CGTTTAACTGTTCCTTCAGCTGTGGGCCCGACTGTTGGAAGCTCTCTCAGTACCCTTGCCTGCAGGTGTA
  CGTGAACCTGACATCTTCCGGGGAGAGGCTCCTCCTCTACCACACGGAAGAGACCATGAAGATCAATCAA
  AAGTGCTCCTATATTCCTAAGTGTGGAAACAACTTTGAGGAGTCCATGTCTCTCGTGAGTGTCGTCATGG
  AAAACTTCAGGAGACACCAACACTTCCCCTGCTATTCTGACCCAGAAGGAAACCAGAAGAGTGTCATCCT
  GACCAAACTCTACAGCTCCAATGTGCTGTTCCATTCTCTCTTCTGGCCAACTTGTATGATGGCTGGGGGT
  GTGGCAATCGTTGCTATGGTGAAACTAACTCAGTACCTCTCCCTGCTTTGTGAGAGGATCCAACGGATCA
  ACAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR202855 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFIWTSGRTSSSYRQDEKRNIYQKIRDHDLLDKRKTVTALKAGEDRAILLGLAMMVCSIMMYFLLGITLL
  RSYMQSVWTEEAQCALLNVSITETFNCSFSCGPDCWKLSQYPCLQVYVNLTSSGERLLLYHTEETMKINQ
  KCSYIPKCGNNFEESMSLVSVVMENFRRHQHFPCYSDPEGNQKSVILTKLYSSNVLFHSLFWPTCMMAGG
  VAIVAMVKLTQYLSLLCERIQRINR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028231
• ORF Size: 708 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028231.2, NP_082507.1
• RefSeq Size: 2947 bp
• RefSeq ORF: 708 bp
• Locus ID: 72413
• UniProt ID: Q9CZM9
• Cytogenetics: 3 A3
• MW: 27.1 kDa
• Gene Summary: Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Acts as a negative regulator that confers rapid and complete inactivation of KCNMA1 channel complex (By similarity).[UniProtKB/Swiss-Prot Function]