Sod2 (NM_013671) Mouse Tagged ORF Clone
Sod2 (Myc-DDK-tagged) - Mouse superoxide dismutase 2, mitochondrial (Sod2), nuclear gene encoding mitochondrial protein
|Product Name||Sod2 (NM_013671) Mouse Tagged ORF Clone|
|Type||Mouse Tagged ORF Clone|
|E. coli Selection||Kanamycin (25 ug/mL)|
>MR202568 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>MR202568 protein sequence
Red=Cloning site Green=Tags(s)
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||669 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at email@example.com or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||3824 bp|
|RefSeq ORF||669 bp|
|Cytogenetics||17 8.75 cM|
|Gene Summary||Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.[UniProtKB/Swiss-Prot Function]|
|The use of this cDNA Clones has been cited in the following citations:|
High expression of MnSOD promotes survival of circulating breast cancer cells and increases their resistance to doxorubicin
,Fu, A;Ma, S;Wei, N;Tan, BX;Tan, EY;Luo, KQ;,
,PubMed ID 27384484
|cDNA Clone Resources|
|MC203718||Sod2 (untagged) - Mouse superoxide dismutase 2, mitochondrial (Sod2), nuclear gene encoding mitochondrial protein, (10ug)||
|MG202568||Sod2 (GFP-tagged) - Mouse superoxide dismutase 2, mitochondrial (Sod2)||
|MR202568L3||Lenti ORF clone of Sod2 (Myc-DDK-tagged) - Mouse superoxide dismutase 2, mitochondrial (Sod2), nuclear gene encoding mitochondrial protein||
|MR202568L4||Lenti ORF clone of Sod2 (mGFP-tagged) - Mouse superoxide dismutase 2, mitochondrial (Sod2), nuclear gene encoding mitochondrial protein||