Txnl4a (BC014770) Mouse Tagged ORF Clone

CAT#: MR202512

(Myc-DDK-tagged) - Mouse cDNA clone MGC:25491 IMAGE:4503212

Product Images

Circular map for MR202512

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Txnl4a
• Synonyms: D18Wsu98e; Dim1; ENSMUSG00000057130; Txnl4; U5-15kD; U5-15kDa
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR202512 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTACATGCTTCCGCATCTGCACAATGGCTGGCAGGTAGACCAGGCCATCCTTTCGGAGGAGGACC
  GCGTGGTCGTCATTCGTTTCGGACACGACTGGGACCCCACTTGCATGAAGATGGACGAGGTTCTGTACAG
  CATCGCCGAAAAGAAATGGAAGATAGTGGGAGATCTTCCTCATCTTGTA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR202512 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSYMLPHLHNGWQVDQAILSEEDRVVVIRFGHDWDPTCMKMDEVLYSIAEKKWKIVGDLPHLV
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: BC014770
• ORF Size: 189 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC014770, AAH14770
• RefSeq Size: 1654 bp
• RefSeq ORF: 662 bp
• Locus ID: 27366
• Cytogenetics: 18 53.34 cM
• MW: 7.4 kDa
• Gene Summary: Plays role in pre-mRNA splicing as component of the U5 snRNP and U4/U6-U5 tri-snRNP complexes that are involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex).[UniProtKB/Swiss-Prot Function]