D1Ertd622e (NM_133825) Mouse Tagged ORF Clone

CAT#: MR202194

D1Ertd622e (Myc-DDK-tagged) - Mouse DNA segment, Chr 1, ERATO Doi 622, expressed (D1Ertd622e)

Product Images

Circular map for MR202194

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: D1Ertd622e
• Synonyms: AI987691; AW047450
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR202194 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGTAGATATAAATGGGGATTCCAGAAGTACCCTGACCACCCTGCCCTTGCCTGTGGCTGAGGGAA
  GCTCTCCAGGAAAAGCAGAGGCAGAGAAACCCCGCTGCTCTAGCACACCCTGCTCGCCCATGCGCCGGAC
  TGTGTCTGGTTATCAGATCCTCCACATGGACTCTAACTACTTGGTTGGCTTCACCACTGGTGAGGAACTC
  CTGAAGTTAGCCCAGAAGTGCACAGGAGGGGAAGACAGCAAGGGAGAAGCCATGCCCGCCTTGCGCGCTA
  AACAGCTGGACACGGGACTCGCGCGTTCCTCTCGTCTGTATAAAACTAGAAGTAGGTACTATCAGCCATA
  TGAGATCCCAGCGGTTAATGGCAGGAGGCGAAGGCGGATGCCCAGCTCAGGAGACAAGTGCACCAAACCC
  TTACCTTATGAACCTTATAAGGCCCTCCATGGGCCCTTGCCTCTATGTCTTCTTAAAGGTAAGAGGGCTC
  ACTCCAAATCTCTGGACTACCTCAATCTAGATAAAATGAACATCAAGGAGCCAGCTGACACGGAAGTGCT
  ACAGTACCAGCTTCAGCACCTGACCCTCCGAGGGGACCGAGTGTTTGCTAGAAACAATACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR202194 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEVDINGDSRSTLTTLPLPVAEGSSPGKAEAEKPRCSSTPCSPMRRTVSGYQILHMDSNYLVGFTTGEEL
  LKLAQKCTGGEDSKGEAMPALRAKQLDTGLARSSRLYKTRSRYYQPYEIPAVNGRRRRRMPSSGDKCTKP
  LPYEPYKALHGPLPLCLLKGKRAHSKSLDYLNLDKMNIKEPADTEVLQYQLQHLTLRGDRVFARNNT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133825
• ORF Size: 624 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133825.3
• RefSeq Size: 2738 bp
• RefSeq ORF: 624 bp
• Locus ID: 52392
• UniProt ID: Q8VEB3
• Cytogenetics: 1 47.76 cM
• MW: 23.1 kDa
• Gene Summary: Probably plays a role in trafficking of proteins via its interaction with UNC119 and UNC119B cargo adapters: may help the release of UNC119 and UNC119B cargo or the recycling of UNC119 and UNC119B. May play a role in ciliary membrane localization via its interaction with UNC119B and protein transport into photoreceptor cells (By similarity).[UniProtKB/Swiss-Prot Function]