Sri (NM_001080974) Mouse Tagged ORF Clone

CAT#: MR201972

Sri (Myc-DDK-tagged) - Mouse sorcin (Sri), transcript variant 1

Product Images

Circular map for MR201972

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sri
• Synonyms: 2210417O06Rik; 2900070H08Rik; Sor
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR201972 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTATCCCGGGCACCCTGGCGCGGGCGGAGGGTACTACCCAGGCGGGTATGGTGGGGCTCCCGGAG
  GGCCTGCGTTCCCTGGACAAACTCAGGATCCGCTATATGGTTACTTTGCTGCTGTGGCTGGACAGGACGG
  ACAAATTGATGCTGACGAATTGCAGAGATGCCTAACACAGTCTGGCATTGCGGGAGGATACAAGCCTTTT
  AACCTGGAGACTTGTCGCCTTATGGTTTCAATGTTGGATAGAGATATGTCCGGCACGATGGGGTTCAATG
  AATTCAAAGAGCTCTGGGCTGTGCTGAATGGCTGGAGACAACACTTCATCAGTTTCGATAGCGATAGGAG
  TGGCACCGTGGATCCCCAGGAGCTACAGAAGGCCCTGACGACTATGGGATTCAGGTTGAGCCCCCAAACT
  GTGAATTCAGTTGCAAAGCGATACAGCACCAGCGGGAAGATCACGTTTGATGACTACATTGCCTGCTGTG
  TCAAACTGAGGGCTCTCACAGATAGCTTTCGCAGACGGGATTCGGGTCAACAAGGAGTTGTGAATTTCTC
  GTATGATGATTTCATCCAGTGTGTCATGACCGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR201972 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAYPGHPGAGGGYYPGGYGGAPGGPAFPGQTQDPLYGYFAAVAGQDGQIDADELQRCLTQSGIAGGYKPF
  NLETCRLMVSMLDRDMSGTMGFNEFKELWAVLNGWRQHFISFDSDRSGTVDPQELQKALTTMGFRLSPQT
  VNSVAKRYSTSGKITFDDYIACCVKLRALTDSFRRRDSGQQGVVNFSYDDFIQCVMTV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001080974
• ORF Size: 597 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080974.2, NP_001074443.1
• RefSeq Size: 2399 bp
• RefSeq ORF: 597 bp
• Locus ID: 109552
• UniProt ID: Q6P069
• Cytogenetics: 5 3.38 cM
• MW: 21.6 kDa
• Gene Summary: Calcium-binding protein that modulates excitation-contraction coupling in the heart. Contributes to calcium homeostasis in the heart sarcoplasmic reticulum. Modulates the activity of RYR2 calcium channels.[UniProtKB/Swiss-Prot Function]