Apopt1 (NM_026511) Mouse Tagged ORF Clone

CAT#: MR201850

Apopt1 (Myc-DDK-tagged) - Mouse RIKEN cDNA 2810002N01 gene (2810002N01Rik), transcript variant 2

Product Images

Circular map for MR201850

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Apopt1
• Synonyms: 1700081D05Rik; 2810002N01Rik; Apop-1; Apop1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR201850 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCTTTGCGGCCCGGAAGCAGAGCGCTTCGCCGGCTTCTCTGCCGGTCCTTCTCCGGCGGCGGCG
  GCGTTAGGTTGGCCCGTGAGCGCCCTACGGATCACAGGGATGCGGCGTCCAGCCGGGTCTCAAGATTCTG
  CCCTCCAAGACAGTCTTGCCATGATTGGATAGGCCCCCCAGACAAATGCTCAAACCTTCGTCCCGTTCAT
  TTTCACATCCCTGAAAACGAGTCTCCCTTGGAGCAAAGACTTAGAGAATTGAGACAAGAAACGCAAGAAT
  GGAATCAACAGTTCTGGGCCAAGCAGAACTTGTCCTTCAATAAGGAAAAAGAAGAATTCATTTACTCAAG
  ACTACAAGCTAAGGGCGCAGGCTTGCGGACTGAGTCAGGCCAAAGAGCAACGCTGGATGCAGAGGAGATG
  GCCGACTTCTATAAGGACTTTCTGAGTAAGAATTTCCAGAAGCACATGCGCTATAACCGAGACTGGTACA
  AGCGTAATTTTGCCATCACTTTCTTCATGGGGAAGGTGGTCCTGGAGAGGATGTGGAGCAAGCTAAGGCA
  AAAGAAGACAAGCAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR201850 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAALRPGSRALRRLLCRSFSGGGGVRLARERPTDHRDAASSRVSRFCPPRQSCHDWIGPPDKCSNLRPVH
  FHIPENESPLEQRLRELRQETQEWNQQFWAKQNLSFNKEKEEFIYSRLQAKGAGLRTESGQRATLDAEEM
  ADFYKDFLSKNFQKHMRYNRDWYKRNFAITFFMGKVVLERMWSKLRQKKTSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026511
• ORF Size: 579 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026511.2, NP_080787.1
• RefSeq Size: 1061 bp
• RefSeq ORF: 579 bp
• Locus ID: 68020
• UniProt ID: Q9CQW7
• Cytogenetics: 12 F1
• MW: 22.7 kDa
• Gene Summary: Plays a role in the regulation of apoptosis. Mediates mitochondria-induced cell death in vascular smooth muscle cells through the release of cytochrome c from mitochondria, followed by the activation of the caspase cascade.[UniProtKB/Swiss-Prot Function]