Dhps (BC039963) Mouse Tagged ORF Clone

CAT#: MR201742

Dhps (Myc-DDK-tagged) - Mouse deoxyhypusine synthase (cDNA clone MGC:49129 IMAGE:4240646)

Product Images

Circular map for MR201742

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dhps
• Synonyms: Dhs, MGC49129, MGC74384
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR201742 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCATTCTGGACCAGATGGTGCTGGAGCAGAACACAGAGGGTGTGAAGTGGACACCTTCCAAGATGA
  TCTCCCGGCTTGGGAAGGAGATCAACAACCCAGACTCTGTGTATTATTGGGCCCATAAGAACCACATCCC
  TGTGCTGAGTCCAGCACTCACGGATGGCTCACTGGGTGACATGATCTTCTTCCATTCCTATAAAAACCCA
  GGCTTGGTCCTGGACATTGTTGAAGACCTGCGACTCATCAACACGCAGGCCATTTTCGCCAAGCGCTCTG
  GGATGATCATCCTGGGTGGAGGTGTGGTCAAGCACCACATTGCCAACGCTAACCTCATGAGGAATGGAGC
  AGACTACGCTGTTTATATCAACACAGCCCAGGAGTTTGATGGCTCAGACTCCGGAGCCCGGCCAGATGAG
  GCTGTCTCTTGGGGCAAGATCCGGATGGACGCACAGCCAGTAAAGGTCTATGCTGATGCTTCTCTGGTTT
  TCCCCTTGCTGGTGGCTGAGACATTCGCCCAAAAGGCAGATGCCTTCAGAGCTGAGAAGAATGAAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR201742 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPILDQMVLEQNTEGVKWTPSKMISRLGKEINNPDSVYYWAHKNHIPVLSPALTDGSLGDMIFFHSYKNP
  GLVLDIVEDLRLINTQAIFAKRSGMIILGGGVVKHHIANANLMRNGADYAVYINTAQEFDGSDSGARPDE
  AVSWGKIRMDAQPVKVYADASLVFPLLVAETFAQKADAFRAEKNED
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC039963
• ORF Size: 558 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC039963, AAH39963
• RefSeq Size: 1149 bp
• RefSeq ORF: 560 bp
• Locus ID: 330817
• Cytogenetics: 8 C3
• MW: 20.6 kDa
• Gene Summary: Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a critical lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue. This is the first step of the post-translational modification of that lysine into an unusual amino acid residue named hypusine. Hypusination is unique to mature eIF-5A factor and is essential for its function.[UniProtKB/Swiss-Prot Function]