Cav2 (NM_016900) Mouse Tagged ORF Clone

CAT#: MR201276

Cav2 (Myc-DDK-tagged) - Mouse caveolin 2 (Cav2)

Product Images

Circular map for MR201276

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cav2
• Synonyms: AI447843
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR201276 representing NM_016900
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCTGGAGACCGAGAAGGCCGATGTGCAGCTCTTCATGGCCGATGACGCCTACAGCCACCACAGTG
  GCGTTGACTACGCAGATCCTGAGAAGTATGTTGACTCGAGTCACGACCGGGATCCTCACCAGCTCAACTC
  TCATCTCAAGCTAGGCTTCGAGGATCTGATTGCAGAGCCTGAGACTACACACTCCTTTGACAAAGTGTGG
  ATCTGCAGCCATGCTCTCTTTGAAATCAGCAAATATGTGATGTACAAGTTCCTGACCGTATTTCTGGCCA
  TCCCCTTGGCCTTCATTGCGGGTATCCTGTTTGCTACCCTCAGCTGTCTGCACATCTGGATCCTGATGCC
  TTTTGTGAAGACCTGCCTAATGGTCTTGCCTTCCGTGCAGACAATATGGAAGAGTGTGACAGACGTTGTC
  ATTGGCCCATTGTGTACAAGTGTGGGCCGCAGCTTCTCATCGGTCAGCATGCAACTGAGCCACGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR201276 representing NM_016900
  Red=Cloning site Green=Tags(s)
  
  MGLETEKADVQLFMADDAYSHHSGVDYADPEKYVDSSHDRDPHQLNSHLKLGFEDLIAEPETTHSFDKVW
  ICSHALFEISKYVMYKFLTVFLAIPLAFIAGILFATLSCLHIWILMPFVKTCLMVLPSVQTIWKSVTDVV
  IGPLCTSVGRSFSSVSMQLSHD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016900
• ORF Size: 486 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016900.4, NP_058596.1
• RefSeq Size: 2748 bp
• RefSeq ORF: 489 bp
• Locus ID: 12390
• UniProt ID: Q9WVC3
• Cytogenetics: 6 A2
• MW: 18.7 kDa
• Gene Summary: This gene belongs to the caveolin family whose members encode the major protein components of caveolae, which are invaginations of plasma membrane. This gene is located adjacent to caveolin-1 and the proteins coexpressed by the two genes localize together in caveolae, where they form hetero-oligomers. The encoded protein may be involved in diverse cellular functions including proliferation, differentiation, endocytosis and trafficking. Alternative splicing of this gene results in transcript variants encoding different isoforms. [provided by RefSeq, Apr 2013]