Kctd9 (NM_134073) Mouse Tagged ORF Clone

CAT#: MR201015

Kctd9 (Myc-DDK-tagged) - Mouse potassium channel tetramerisation domain containing 9 (Kctd9), transcript variant 2

Product Images

Circular map for MR201015

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kctd9
• Synonyms: AA675328; AI854539
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR201015 representing NM_134073
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGCGGGTCACGCTGTTCTTGAACGGCAGCCCGAGCAACGGCAAGGTGGTTGCTGTATACGGAACTT
  TGTCTGACTTGCTCTCAGTGGCCAGCAGTAAACTCGGCATAAAAGCCACCAGTGTATATAATGGGAAAGG
  CGGACTGATTGATGATATTGCTTTGATCAGGGATGATGATGTGCTGTTTGTGTGTGAAGGAGAGCCGTTT
  ATTGATCCTCAGACAGATGCTAGACCACCTGGAGGATTGCTAGGATCCCACACAGACTGGCTCACATTAA
  ATGTAGGAGGACGATACTTCACAACAACAAGGAGCACTTTAGTGAATAAAGAGCCTGACAGTATGCTGGC
  GCACATGTTCAAGGACAAAGGTGTCTGGGGAAATAAGCAAGATCATAGAGGAGCATTCTTAATTGACCGA
  AGTCCTGAGTACTTTGAACCTATTTTGAACTACTTACGTCATGGACAGCTCATTGTAAATGATGGCATTA
  ACTTATTGGGTGTGCTAGAAGAAGCCAGGTTTTTTGGTATTGACTCACTGATTGAACACCTAGAAGTGGC
  CATAAAGAATTCCCAACCACCAGAGGATCATTCACCAATATCCCGAAAGGAATTTGTTCGATTTCTGCTG
  GCCACTCCAACCAAGTCAGAGCTGCGCTGCCAGTGTGCAAATCTCCAGGGAGTCAAGATGCTCTGCTCAA
  ACGCAGAAGGCGCATCCCTGAGGCTCTGTAATTTTGAGGATCCTTCGGGTCTTAAAGCCAACTTAGAGGG
  TGCTAATCTGAAAGGTGTGGACATGGAAGGAAGCCAGATGACAGGGATTAACCTCAGAGTTGCCACCTTA
  AAAAATGCAAAACTGAAGAACTGTAATCTCAGAGGAGCAACTCTGGCAGGAACCGATTTAGAAAACTGTG
  ATTTGTCGGGGTGTGACCTCCAGGAAGCCAACCTGAGAGGCTCCAATGTGAAGGGTGCCATATTTGAAGA
  GATGCTGACACCATTACATATGTCCCAGAGTGTCAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR201015 representing NM_134073
  Red=Cloning site Green=Tags(s)
  
  MRRVTLFLNGSPSNGKVVAVYGTLSDLLSVASSKLGIKATSVYNGKGGLIDDIALIRDDDVLFVCEGEPF
  IDPQTDARPPGGLLGSHTDWLTLNVGGRYFTTTRSTLVNKEPDSMLAHMFKDKGVWGNKQDHRGAFLIDR
  SPEYFEPILNYLRHGQLIVNDGINLLGVLEEARFFGIDSLIEHLEVAIKNSQPPEDHSPISRKEFVRFLL
  ATPTKSELRCQCANLQGVKMLCSNAEGASLRLCNFEDPSGLKANLEGANLKGVDMEGSQMTGINLRVATL
  KNAKLKNCNLRGATLAGTDLENCDLSGCDLQEANLRGSNVKGAIFEEMLTPLHMSQSVR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_134073
• ORF Size: 1017 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134073.2, NP_598834.2
• RefSeq Size: 3174 bp
• RefSeq ORF: 1020 bp
• Locus ID: 105440
• UniProt ID: Q80UN1
• Cytogenetics: 14 D1
• MW: 37.4 kDa
• Gene Summary: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex, which mediates the ubiquitination of target proteins, leading to their degradation by the proteasome.[UniProtKB/Swiss-Prot Function]