Tsc22d3 (NM_010286) Mouse Tagged ORF Clone

CAT#: MR200843

Tsc22d3 (Myc-DDK-tagged) - Mouse TSC22 domain family, member 3 (Tsc22d3), transcript variant 2

Product Images

Circular map for MR200843

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tsc22d3
• Synonyms: DIP; Dsip1; Gilz; Tilz3; TSC-22R
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR200843 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACACCGAAATGTATCAGACCCCCATGGAGGTGGCGGTCTATCAGCTGCACAATTTCTCCATCTCCT
  TCTTTTCTTCTCTGCTTGGAGGGGATGTGGTTTCCGTTAAACTGGATAACAGTGCCTCCGGAGCCAGTGT
  GGTGGCCCTAGACAACAAGATTGAGCAGGCCATGGACCTCGTGAAGAACCACCTGATGTACGCTGTGAGA
  GAGGAGGTGGAGGTCCTAAAGGAGCAGATTCGTGAGCTGCTTGAGAAGAACTCCCAGCTGGAGCGCGAGA
  ACACCCTCCTGAAGACGCTGGCAAGCCCCGAGCAACTGGAAAAGTTCCAGTCCCGGCTGAGCCCTGAAGA
  GCCAGCACCTGAAGCCCCAGAAACCCCGGAAACCCCGGAAGCCCCTGGTGGTTCTGCGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR200843 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNTEMYQTPMEVAVYQLHNFSISFFSSLLGGDVVSVKLDNSASGASVVALDNKIEQAMDLVKNHLMYAVR
  EEVEVLKEQIRELLEKNSQLERENTLLKTLASPEQLEKFQSRLSPEEPAPEAPETPETPEAPGGSAV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010286
• ORF Size: 414 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010286.5
• RefSeq Size: 1974 bp
• RefSeq ORF: 414 bp
• Locus ID: 14605
• UniProt ID: Q9Z2S7
• Cytogenetics: X F1
• MW: 15.2 kDa
• Gene Summary: Protects T-cells from IL2 deprivation-induced apoptosis through the inhibition of FOXO3A transcriptional activity that leads to the down-regulation of the pro-apoptotic factor BCL2L11. In macrophages, plays a role in the anti-inflammatory and immunosuppressive effects of glucocorticoids and IL10. In T-cells, inhibits anti-CD3-induced NFKB1 nuclear translocation. In vitro, suppresses AP1 and NFKB1 DNA-binding activities (By similarity). Isoform 1 and isoform 4 inhibit myogenic differentiation and mediate anti-myogenic effects of glucocorticoids by binding and regulating MYOD1 and HDAC1 transcriptional activity resulting in reduced expression of MYOG.[UniProtKB/Swiss-Prot Function]