Havcr1 (NM_001166631) Mouse Tagged ORF Clone

CAT#: MG227390

Havcr1 (tGFP-tagged) - Mouse hepatitis A virus cellular receptor 1 (Havcr1) transcript variant 2, (10ug)

Product Images

Circular map for MG227390

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Havcr1
• Synonyms: AI503787; KIM-1; TIM-1; Tim1; Timd1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227390 representing NM_001166631
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCAGATTCAAGTCTTCATTTCAGGCCTCATACTGCTTCTCCCAGGCGCTGTGGATTCTTATGTGG
  AAGTAAAGGGGGTGGTGGGTCACCCTGTCACACTTCCATGTACTTACTCAACATATCGTGGAATCACAAC
  GACATGTTGGGGCCGAGGGCAATGCCCATCTTCTGCTTGTCAAAATACACTTATTTGGACCAATGGACAT
  CGTGTCACCTATCAGAAGAGCAGTCGGTACAACTTAAAGGGGCATATTTCAGAAGGAGATGTGTCCTTGA
  CGATAGAGAACTCTGTTGAGAGTGACAGTGGTCTGTATTGTTGTCGAGTGGAGATTCCTGGATGGTTTAA
  TGATCAGAAAGTGACCTTTTCATTGCAAGTTAAACCAGAGATTCCCACACGTCCTCCAAGAAGACCCACA
  ACTACAAGGCCCACAGCTACAGGAAGACCCACGGCTATTTCAACAAGATCCACACATGTACCAACATCAA
  CCAGAGTCTCTACCTCCACTCCTCCAACATCTACACACACATGGACTCACAAACCAGACTGGAATGGCAC
  TGTGACATCCTCAGGAGATACCTGGAGTAATCACACTGAAGCAATCCCTCCAGGGAAGCCGCAGAAAAAC
  CCTACTAAGGGCTTCTATGTTGGCATCTGCATCGCAGCCCTGCTGCTACTGCTCCTTGTGAGCACCGTGG
  CTATCACCAGGTACATACTTATGAAAAGGAAGTCAGCATCTCTAAGCGTGGTTGCCTTCCGTGTCTCTAA
  GATTGAAGCTTTGCAGAACGCAGCGGTTGTGCATTCCCGAGCTGAAGACAACATCTACATTGTTGAAGAT
  AGACCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227390 representing NM_001166631
  Red=Cloning site Green=Tags(s)
  
  MNQIQVFISGLILLLPGAVDSYVEVKGVVGHPVTLPCTYSTYRGITTTCWGRGQCPSSACQNTLIWTNGH
  RVTYQKSSRYNLKGHISEGDVSLTIENSVESDSGLYCCRVEIPGWFNDQKVTFSLQVKPEIPTRPPRRPT
  TTRPTATGRPTAISTRSTHVPTSTRVSTSTPPTSTHTWTHKPDWNGTVTSSGDTWSNHTEAIPPGKPQKN
  PTKGFYVGICIAALLLLLLVSTVAITRYILMKRKSASLSVVAFRVSKIEALQNAAVVHSRAEDNIYIVED
  RP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001166631
• ORF Size: 846 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166631.1, NP_001160103.1
• RefSeq Size: 2001 bp
• RefSeq ORF: 849 bp
• Locus ID: 171283
• UniProt ID: Q5QNS5
• Cytogenetics: 11 B1.1
• Gene Summary: May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4. May play a role in kidney injury and repair (By similarity).[UniProtKB/Swiss-Prot Function]