Alox5 (NM_009662) Mouse Tagged ORF Clone

CAT#: MG227106

Alox5 (tGFP-tagged) - Mouse arachidonate 5-lipoxygenase (Alox5), (10ug)

Product Images

Circular map for MG227106

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Alox5
• Synonyms: 5-LO; 5-LOX; 5LO; 5LX; AI850497; F730011J02
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227106 representing NM_009662
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTCCTACACGGTCACCGTGGCCACCGGCAGCCAGTGGTTCGCGGGCACCGACGACTACATCTACC
  TCAGCCTCATTGGCTCTGCGGGCTGTAGCGAGAAGCATCTGCTGGACAAGGCATTCTACAATGACTTCGA
  ACGGGGCGCGGTGGACTCCTACGATGTCACCGTGGATGAGGAGCTGGGCGAGATCTACCTAGTCAAAATT
  GAGAAGCGCAAATACTGGCTCCATGATGACTGGTACCTGAAGTACATCACACTGAAGACACCCCACGGGG
  ACTACATCGAGTTCCCATGTTACCGCTGGATCACAGGCGAGGGCGAGATTGTCCTGAGGGATGGACGTGC
  AAAATTGGCCCGAGATGACCAAATTCACATCCTCAAGCAGCACAGACGTAAAGAACTGGAGGCACGGCAA
  AAACAGTATCGATGGATGGAGTGGAACCCCGGCTTCCCTTTGAGTATTGATGCCAAATGCCACAAGGATC
  TGCCCCGAGATATCCAGTTTGATAGTGAAAAAGGAGTGGACTTTGTTCTGAACTACTCAAAAGCGATGGA
  GAACCTGTTCATCAACCGCTTCATGCACATGTTCCAGTCTTCCTGGCACGACTTTGCTGACTTTGAGAAA
  ATCTTCGTCAAAATCAGCAACACTATATCTGAGCGAGTCAAGAACCACTGGCAGGAAGACCTCATGTTTG
  GCTACCAGTTCCTGAATGGCTGCAACCCAGTACTCATCAAGCGCTGCACAGCGTTGCCCCCGAAGCTCCC
  AGTGACCACAGAGATGGTGGAGTGCAGCCTAGAGCGGCAGCTCAGTTTAGAACAGGAAGTACAGGAAGGG
  AACATTTTCATCGTTGATTACGAGCTACTGGATGGCATCGATGCTAACAAAACTGACCCCTGTACACACC
  AGTTCCTGGCTGCCCCCATCTGCCTGCTATATAAGAACCTAGCCAACAAGATTGTTCCCATTGCCATCCA
  GCTCAACCAAACCCCTGGAGAGAGTAACCCAATCTTCCTCCCTACGGATTCAAAGTACGACTGGCTTTTG
  GCCAAAATCTGGGTGCGTTCCAGTGACTTCCACGTCCATCAAACGATCACCCACCTTCTGCGCACGCATC
  TGGTGTCTGAGGTGTTTGGTATCGCCATGTACCGCCAGCTGCCTGCTGTGCATCCCCTTTTCAAGCTGCT
  GGTAGCCCATGTGAGGTTCACCATTGCTATCAACACTAAGGCCCGGGAACAGCTTATCTGCGAGTATGGC
  CTTTTTGACAAGGCCAATGCCACCGGGGGTGGAGGGCACGTGCAGATGGTGCAGAGGGCTGTCCAGGATC
  TGACCTATTCCTCCCTGTGTTTCCCGGAGGCCATCAAGGCCCGGGGCATGGACAGCACGGAAGACATCCC
  CTTCTACTTCTATCGTGATGATGGACTGCTCGTGTGGGAGGCTATCCAGTCGTTCACAATGGAGGTGGTG
  AGCATCTACTATGAGAACGACCAGGTGGTGGAGGAGGACCAGGAACTGCAGGACTTCGTGAAGGATGTTT
  ACGTGTACGGCATGCGGGGCAAAAAGGCCTCAGGTTTCCCCAAGTCCATCAAGAGCAGGGAGAAGCTGTC
  CGAGTACCTGACGGTGGTGATCTTCACGGCCTCTGCCCAGCATGCAGCTGTAAACTTCGGCCAGTATGAC
  TGGTGCTCCTGGATCCCCAACGCTCCTCCAACTATGCGGGCCCCACCACCCACGGCCAAGGGTGTGGTCA
  CCATCGAGCAGATCGTGGATACTCTACCAGACCGTGGCCGATCATGTTGGCATCTAGGTGCAGTGTGGGC
  CTTGAGCCAGTTTCAAGAAAATGAGCTGTTTCTAGGCATGTACCCAGAGGAGCATTTCATTGAGAAGCCA
  GTGAAGGAAGCCATGATCCGATTCCGCAAGAACCTGGAGGCCATCGTCAGCGTGATCGCCGAGCGCAATA
  AGAACAAAAAGCTCCCCTACTACTACCTGTCACCAGACAGGATTCCCAACAGCGTAGCCATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227106 representing NM_009662
  Red=Cloning site Green=Tags(s)
  
  MPSYTVTVATGSQWFAGTDDYIYLSLIGSAGCSEKHLLDKAFYNDFERGAVDSYDVTVDEELGEIYLVKI
  EKRKYWLHDDWYLKYITLKTPHGDYIEFPCYRWITGEGEIVLRDGRAKLARDDQIHILKQHRRKELEARQ
  KQYRWMEWNPGFPLSIDAKCHKDLPRDIQFDSEKGVDFVLNYSKAMENLFINRFMHMFQSSWHDFADFEK
  IFVKISNTISERVKNHWQEDLMFGYQFLNGCNPVLIKRCTALPPKLPVTTEMVECSLERQLSLEQEVQEG
  NIFIVDYELLDGIDANKTDPCTHQFLAAPICLLYKNLANKIVPIAIQLNQTPGESNPIFLPTDSKYDWLL
  AKIWVRSSDFHVHQTITHLLRTHLVSEVFGIAMYRQLPAVHPLFKLLVAHVRFTIAINTKAREQLICEYG
  LFDKANATGGGGHVQMVQRAVQDLTYSSLCFPEAIKARGMDSTEDIPFYFYRDDGLLVWEAIQSFTMEVV
  SIYYENDQVVEEDQELQDFVKDVYVYGMRGKKASGFPKSIKSREKLSEYLTVVIFTASAQHAAVNFGQYD
  WCSWIPNAPPTMRAPPPTAKGVVTIEQIVDTLPDRGRSCWHLGAVWALSQFQENELFLGMYPEEHFIEKP
  VKEAMIRFRKNLEAIVSVIAERNKNKKLPYYYLSPDRIPNSVAI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009662
• ORF Size: 2022 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009662.2, NP_033792.1
• RefSeq Size: 2827 bp
• RefSeq ORF: 2025 bp
• Locus ID: 11689
• UniProt ID: P48999
• Cytogenetics: 6 53.79 cM
• Gene Summary: Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.[UniProtKB/Swiss-Prot Function]