Alox5 (NM_009662) Mouse Untagged Clone

CAT#: MC220311

Alox5 (untagged) - Mouse arachidonate 5-lipoxygenase (Alox5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Alox5
• Synonyms: 5-LO; 5-LOX; 5LO; 5LX; AI850497; F730011J02
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220311 representing NM_009662
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTCCTACACGGTCACCGTGGCCACCGGCAGCCAGTGGTTCGCGGGCACCGACGACTACATCTACC
  TCAGCCTCATTGGCTCTGCGGGCTGTAGCGAGAAGCATCTGCTGGACAAGGCATTCTACAATGACTTCGA
  ACGGGGCGCGGTGGACTCCTACGATGTCACCGTGGATGAGGAGCTGGGCGAGATCTACCTAGTCAAAATT
  GAGAAGCGCAAATACTGGCTCCATGATGACTGGTACCTGAAGTACATCACACTGAAGACACCCCACGGGG
  ACTACATCGAGTTCCCATGTTACCGCTGGATCACAGGCGAGGGCGAGATTGTCCTGAGGGATGGACGTGC
  AAAATTGGCCCGAGATGACCAAATTCACATCCTCAAGCAGCACAGACGTAAAGAACTGGAGGCACGGCAA
  AAACAGTATCGATGGATGGAGTGGAACCCCGGCTTCCCTTTGAGTATTGATGCCAAATGCCACAAGGATC
  TGCCCCGAGATATCCAGTTTGATAGTGAAAAAGGAGTGGACTTTGTTCTGAACTACTCAAAAGCGATGGA
  GAACCTGTTCATCAACCGCTTCATGCACATGTTCCAGTCTTCCTGGCACGACTTTGCTGACTTTGAGAAA
  ATCTTCGTCAAAATCAGCAACACTATATCTGAGCGAGTCAAGAACCACTGGCAGGAAGACCTCATGTTTG
  GCTACCAGTTCCTGAATGGCTGCAACCCAGTACTCATCAAGCGCTGCACAGCGTTGCCCCCGAAGCTCCC
  AGTGACCACAGAGATGGTGGAGTGCAGCCTAGAGCGGCAGCTCAGTTTAGAACAGGAAGTACAGGAAGGG
  AACATTTTCATCGTTGATTACGAGCTACTGGATGGCATCGATGCTAACAAAACTGACCCCTGTACACACC
  AGTTCCTGGCTGCCCCCATCTGCCTGCTATATAAGAACCTAGCCAACAAGATTGTTCCCATTGCCATCCA
  GCTCAACCAAACCCCTGGAGAGAGTAACCCAATCTTCCTCCCTACGGATTCAAAGTACGACTGGCTTTTG
  GCCAAAATCTGGGTGCGTTCCAGTGACTTCCACGTCCATCAAACGATCACCCACCTTCTGCGCACGCATC
  TGGTGTCTGAGGTGTTTGGTATCGCCATGTACCGCCAGCTGCCTGCTGTGCATCCCCTTTTCAAGCTGCT
  GGTAGCCCATGTGAGGTTCACCATTGCTATCAACACTAAGGCCCGGGAACAGCTTATCTGCGAGTATGGC
  CTTTTTGACAAGGCCAATGCCACCGGGGGTGGAGGGCACGTGCAGATGGTGCAGAGGGCTGTCCAGGATC
  TGACCTATTCCTCCCTGTGTTTCCCGGAGGCCATCAAGGCCCGGGGCATGGACAGCACGGAAGACATCCC
  CTTCTACTTCTATCGTGATGATGGACTGCTCGTGTGGGAGGCTATCCAGTCGTTCACAATGGAGGTGGTG
  AGCATCTACTATGAGAACGACCAGGTGGTGGAGGAGGACCAGGAACTGCAGGACTTCGTGAAGGATGTTT
  ACGTGTACGGCATGCGGGGCAAAAAGGCCTCAGGTTTCCCCAAGTCCATCAAGAGCAGGGAGAAGCTGTC
  CGAGTACCTGACGGTGGTGATCTTCACGGCCTCTGCCCAGCATGCAGCTGTAAACTTCGGCCAGTATGAC
  TGGTGCTCCTGGATCCCCAACGCTCCTCCAACTATGCGGGCCCCACCACCCACGGCCAAGGGTGTGGTCA
  CCATCGAGCAGATCGTGGATACTCTACCAGACCGTGGCCGATCATGTTGGCATCTAGGTGCAGTGTGGGC
  CTTGAGCCAGTTTCAAGAAAATGAGCTGTTTCTAGGCATGTACCCAGAGGAGCATTTCATTGAGAAGCCA
  GTGAAGGAAGCCATGATCCGATTCCGCAAGAACCTGGAGGCCATCGTCAGCGTGATCGCCGAGCGCAATA
  AGAACAAAAAGCTCCCCTACTACTACCTGTCACCAGACAGGATTCCCAACAGCGTAGCCATCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009662
• Insert Size: 2025 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009662.2, NP_033792.1
• RefSeq Size: 2827 bp
• RefSeq ORF: 2025 bp
• Locus ID: 11689
• UniProt ID: P48999
• Cytogenetics: 6 53.79 cM
• Gene Summary: Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.[UniProtKB/Swiss-Prot Function]