Mki67 (NM_001081117) Mouse Tagged ORF Clone

CAT#: MG227041

Mki67 (tGFP-tagged) - Mouse antigen identified by monoclonal antibody Ki 67 (Mki67), (10ug). Note: ORF is codon optimized

Product Images

Circular map for MG227041

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mki67
• Synonyms: D630048A14Rik; Ki-67; Ki67
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227041 representing NM_001081117, codon optimized.
  Due to the complexity of NM_001081117, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCATCTGCCCACCTGGTAACAATAAAGAGGTCCGGCGACGACGGAGCCCATTTTCCTCTGTCTC
  TGAGTAGCTGCCTCTTCGGCAGGTCCATTGAGTGCGACATTAGAATCCAGCTGCCCGTGGTGAGCAAGAG
  GCACTGCAAGATAGAAGTGAAGGAACAGGAAGCCATCCTGTACAACTTTTCTTCAACAAACCCCACCCAG
  GTAAACGGCGTGACAATCGATGAGCCTGTAAGGCTGAGGCACGGCGATATCATCACCATAATCGACCGGT
  CCTTCAGATACGAAGATGGTAACCATGAAGACGGGTCTAAGCCTACCGAGTTCCCAGGCAAAAGCTTGGG
  TAAAGAACCCAGCCGGCGCGCTAGTAGGGATAGTTTTTGCGCGGACCCGGATGGCGAAGGTCAGGATACA
  AAAGCTAGCAAGATGACCGCCTCCCGAAGATCATTTGTGTATGCGAAAGGGCTCAGTGCAGACTCCCCAG
  CTTCCGACGGAAGTAAAAACAGTGTGTCTCAGGACTCATCTGGTCACGTGGAACAGCACACTGGCAGGAA
  CATCGTGGAGCCGACATCTGGGGATCTGTTCAAGAAGTCCCGGTCAACAGGTTCAAGCTACCGAGAGCCA
  AAATCTTCTCCTACTCAGAGCTTGTCTAATTCTAACGAAAAGGAATCTCCCTTCGAGAAGCTCTACCAGT
  CTATGAAAGAGGAGCTGGATGTCAAGTCTCAGAAGAGTTGCCGAAAGAGCGAACCCCAGCCGGATAGAGC
  AGCCGAGGAGAGCCGCGAGACTCAGCTGCTCGTGAGCGGCAGGGCCAGAGCGAAGTCATCAGGATCTACC
  CCTGTGACTGCCGCATCTTCCCCAAAAGTGGGAAAGATCTGGACTGAGCGCTGGAGGGGGGGGATGGTCC
  CAGTCCAGACCTCTACCGAAACAGCAAAGATGAAGACCCCAGTCCGCCACTCTCAGCAGCTCAAAGACGA
  GGATTCTAGAGTCACAGGGAGAAGGCACAGTGTCAACCTGGACGAGGGGGAGTCCGCACAGGCTGTGCAC
  AAGACAGTCACACCGGGTAAGCTGGCGACTAGGAATCAGGCAGCCGTTGAGGCTGGGGACGTGGCATCCC
  CCGCTGACACTCCCGAGCATTCAAGTTCCAAAAAGAGATCTATTCCCGCCAAGGTGGAAGCTCCTTCCGC
  CGAGACACAAAAGCGCCTGTCTCTCACGCAGCGCCTGGTACCCGGGGAGAAGAAGACACCAAAGGGCTCT
  TTCAGTAAGCCAGAGAAGCTGGCCACCGCAGCCGAACAGACCTGTTCCGGTCTGCCTGGGCTGAGCAGCG
  TGGACATTTCTAACTTCGGAGACAGTATCAACAAGAGCGAGGGAATGCCAATGAAGCGACGGAGGGTGAG
  CTTCGGTGGCCATCTGCGACCTGAGTTGTTCGATGAGAATCTTCCTCCTAACACCCCACTGAAGCGGGGC
  GAAACACCGACCAAACGGAAAAGCCTCGGGACCCACAGTCCCGCTGTGCTGAAGACGATCATCAAGGAAC
  GACCGCAAAGCCCTGGCAAACAGGAAAGCCCTGGTATTACTCCCCCAAGGACGAATGACCAGCGCAGGAG
  GAGCGGTCGGACCAGTAGTGGTTCTAAGTTCTTGTGTGAAACAGATATACCGAAGAAAGCCGGTAGGAAG
  AGTGGTAACCTCCCAGCAAAAAGAGCTTCTATCAGCCGGTCCCAGCACGGGATCCTCCAGATGATTTGTA
  GCAAGAGGCGCTCTGGCGCCAGCGAGGCCAACTTGATCGTCGCCAAGTCCTGGGCAGATGTTGTGAAGCT
  CGGGGTTAAACAGACCCAGACAAAGGTCGCGAAACACGTGCCCCAAAAACAGACTTCTAAGAGGCAGCGC
  AGGCCAAGTACACCAAAGAAGCCAACAAGCAATCTGCACAACCAGTTCACAACTGGCCACGCCAACTCTC
  CGTGCACTATCGTGGTGGGTAGAGCACAGATCGAGAAGGTGTCAGTGCCTGCAAGGCCTTATAAAATGCT
  GAACAACCTGATGCTCAATAGGAAAGTAGACTTCTCCGAAGACCTGAGCGGCCTGACCGAAATGTTCAAG
  ACACCCGTTAAGGAGAAGCAGCAGCAGATGTCCGACACAGGATCAGTGCTGTCAAATTCCGCCAACCTTT
  CTGAAAGACAGTTGCAAGTAACAAATTCTGGGGACATACCGGAGCCCATCACAACTGAAATCCTCGGCGA
  AAAAGTACTTTCAAGTACTCGAAATGCAGCGAAGCAACAATCAGATCGGTATAGCGCCAGCCCGACACTG
  CGGCGCCGATCAATAAAGCATGAGAATACCGTGCAGACCCCCAAGAACGTTCACAACATCACAGACCTTG
  AAAAGAAGACACCCGTCTCCGAAACAGAGCCACTTAAAACGGCTTCATCCGTGAGTAAGCTGCGCCGGAG
  CAGGGAACTTCGCCATACACTTGTGGAAACGATGAACGAGAAGACAGAGGCTGTGCTGGCTGAAAATACT
  ACCGCGCGACATCTGAGAGGGACATTTCGCGAGCAGAAGGTGGACCAACAAGTCCAGGACAACGAAAATG
  CCCCACAGCGGTGCAAGGAGTCTGGTGAGTTGTCAGAGGGAAGTGAGAAGACTAGTGCCAGACGCTCCTC
  TGCTCGCAAGCAGAAACCAACTAAGGACCTCCTGGGGAGCCAGATGGTCACGCAGACAGCTGATTACGCA
  GAGGAACTGCTGAGTCAGGGCCAGGGGACAATTCAGAATCTCGAGGAGTCCATGCATATGCAGAACACCT
  CTATCTCAGAGGATCAGGGGATAACAGAGAAGAAAGTGAACATCATCGTGTATGCTACTAAGGAAAAGCA
  CAGTCCAAAGACGCCAGGGAAAAAGGCGCAGCCTCTTGAAGGTCCCGCCGGATTGAAAGAGCACTTCGAG
  ACGCCGAACCCTAAAGACAAGCCTATCACCGAGGATCGCACCCGGGTCCTGTGTAAAAGCCCGCAGGTGA
  CAACCGAGAACATTACCACGAACACTAAGCCGCAGACATCTACAAGCGGCAAGAAGGTGGATATGAAGGA
  AGAATCCTCCGCACTGACGAAACGAATCCATATGCCCGGGGAGTCTCGACACAACCCCAAAATACTGAAG
  TTGGAGTGCGAAGATATAAAGGCTCTGAAGCAATCCGAAAATGAGATGCTGACCAGTACAGTGAATGGAT
  CAAAGCGAACCCTGGAAAAAAGCAAGAAAAAAGCTCAGCCCCTTGAGGACCTCACCTGCTTCCAGGAACT
  GTTCATCTCTCCCGTGCCAACTAACATCATAAAGAAGATTCCTTCCAAATCCCCACACACTCAGCCGGTG
  CGGACTCCTGCTAGCACAAAGAGGCTGTCCAAGACTGGCTTGAGTAAGGTGGATGTACGCCAGGAACCAA
  GCACTCTGGGAAAACGGACAAAGAGCCCTGGTAGGGCACCCGGTACTCCCGCCCCCGTGCAAGAGGAGAA
  CGATAGCACAGCTTTCATGGAAACGCCTAAGCAAAAACTGGACTTCGCAGGTAACAGCAGCGGCTCCAAA
  CGGAGATCTCGAACTTCAAAGAACAGAAGTCAACCCTTGGAGGACCTCGATGGCTTCCAGGAGCTGTTCC
  AGACGCCGGCCGGAGCATCTGACTCCGTGACCGTCGAGGAGTCAGCCAAAATAAGTCTCGAGAGTTCCCA
  AGCTGAACCGGTTAAGACTCCCGCTAGCACAAAGAGAAGATCTAAAATGTCTCTGATGAAAGTGGACATG
  AAGGAGCTGAGCATACTCGAAAAGCAAACGCAAAGTCGGGGTCGAGATGCAGGAACGCCAGCGCCCATGC
  AGGAAGGCAATGGAACAACAGCAATAATGGAGACACCTAAACAGAAGCTGGATTTTACCGGGAACAGCAC
  AGGACATAAGAGGAGGCCAAGAACGCCCAAAATCAGAGCTCAACCCCTGGAGGATCTTGATGGATTCCAG
  GAGCTGTTCCAAACCCCCGCAGGTGCTAACGACTCTGTGACAGTTGAAGAGTCTGCCAAGATGAGTCTTG
  AGAGTAGTCAGGCAGAACCAGTGAAGACACCAGCCTCAACCAAACGCTTGAGTAAAACTGATCTCAGTAA
  AGTGGACGTGCGAGAAGATCCCAGTATCCTGGGGAAGAAGACAAAGTCTCCCGGGAGGGCTCCAGGCACA
  CCAGCCCCAGTTCAGGAAGAGAACGACTGTACCGCTTACATGGAGACTCCCAAGCAGAAACTGGAGTCCA
  TAGAAAACCTGACAGGCCTTAGGAAGCAGTCAAGGACACCAAAGGACATCACCGGATTCCAAGATTCATT
  CCAGATCCCTGACCATGCAAATGGCCCTCTGGTCGTAGTCAAGACCAAGAAGATGTTCTTTAACTCACCA
  CAGCCAGAATCCGCGATAACACGCAAATCACGGGAAAGGCAGAGCAGGGCGAGTATCAGTAAGATAGATG
  TAAAAGAAGAGCTGCTCGAATCTGAAGAACATCTTCAGCTGGGCGAGGGCGTCGACACCTTTCAGGTGTC
  TACTAACAAGGTTATCCGGTCCTCCAGGAAACCTGCTAAAAGGAAGCTGGACAGTACTGCCGGTATGCCT
  AATTCTAAGAGGATGAGATGCTCCTCCAAGGATAACACCCCTTGTCTTGAAGATCTGAATGGGTTCCAGG
  AACTTTTCCAGATGCCAGGCTACGCTAATGACAGTCTTACGACCGGCATCTCTACGATGCTGGCGCGCTC
  ACCCCAGCTCGGTCCTGTAAGGACTCAGATTAACAAGAAGAGCCTTCCAAAAATCATTCTGCGCAAGATG
  GACGTGACCGAGGAGATTAGTGGTCTGTGGAAGCAGTCCCTTGGCAGGGTTCACACTACCCAGGAACAGG
  AGGACAACGCTATCAAAGCCATCATGGAAATTCCTAAAGAGACTCTCCAGACTGCAGCAGACGGCACTCG
  GCTGACCAGGCAGCCCCAGACCCCCAAAGAGAAAGTCCAACCTCTCGAGGACCACAGCGTGTTCCAGGAA
  CTCTTTCAGACTAGCCGATACTGCTCCGACCCTTTGATTGGTAATAAGCAGACTAGGATGAGCTTGCGCT
  CCCCCCAGCCTGGATTCGTGAGGACCCCACGCACCTCAAAAAGACTCGCCAAAACTTCTGTAGGCAACAT
  TGCCGTGAGAGAGAAAATATCTCCCGTGTCACTTCCCCAGTGCGCCACGGGTGAGGTAGTCCACATCCCC
  ATAGGCCCCGAAGATGATACGGAGAACAAGGGCGTTAAGGAATCAACACCACAGACTTTGGACTCATCCG
  CTAGTAGGACAGTGTCAAAGCGACAACAGGGAGCCCACGAGGAAAGACCACAATTCAGTGGGGACCTGTT
  CCATCCGCAAGAACTCTTTCAGACGCCCGCCTCAGGCAAAGATCCAGTGACCGTGGACGAAACAACGAAA
  ATCGCCCTTCAGTCCCCCCAGCCAGGACACATTATAAATCCAGCAAGTATGAAGCGGCAATCCAATATGA
  GTCTCAGGAAGGACATGCGCGAGTTCTCTATTCTCGAAAAGCAAACTCAGTCACGCGGAAGGGACGCCGG
  AACTCCCGCGCCTATGCAGGAGGAGAACGGGACAACAGCAATAATGGAAACCCCGAAACAGAAGCTCGAC
  TTTATCGGGAACTCCACAGGCCACAAGCGACGGCCTCGCACACCCAAAAATAGGGCTCAGCCCCTTGAGG
  ACCTTGACGGATTTCAGGAGCTGTTTCAGACGCCCGCTGGGGCTTCCGACCCTGTTTCCGTGGAGGAATC
  AGCGAAGATCTCCCTGGCATCTTCACAGGCCGAGCCAGTCAGGACCCCCGCCAGCACCAAGCGACGCAGC
  AAGACTGGACTTAGTAAGGTCGATGTTAGACAAGAGCCTAGCACGCTGGGAAAACGCATGAAATCTCTGG
  GAAGAGCTCCGGGTACGCCAGCACCCGTGCAGGAGGAGAACGACAGCACCGCCTTCATGGAGACTCCAAA
  ACAGAAGCTGGACTTCACCGGAAACAGTAGCGGTCATAAGCGACGACCACAGACGCCAAAGATTAGGGCA
  CAGCCTTTGGAAGACCTTGATGGCTTCCAGGAGCTGTTCCAGACGCCAGCGGGTGCTAACGATTCTGTTA
  CAGTCGAGGAGTCAGTGAAGATGTCACTGGAATCCTCACAAGCTGAGCCTGTGAAAACACCCGCCTCTAC
  CAAGAGATTGTCAAAAACTGGCTTGTCAAAGGTGGATGTGCGGGAAGATCCCAGCATCCTGGAAAAAAAG
  ACAAAGTCTCCCGGTACCCCCGCCCCCGTGCAGGAGGAAAATGATTGTACCGCCTTCATGGAGACACCAA
  AGCAGAAACTCGATTTTACCGGGAATAGCTCAGGTCACAAGCGACGGCCCAGGACCCCCAAGATCAGGGC
  TCAGCCCCTCGAAGACCTTGATGGGTTCCAGGAGCTGTTCCAGACCCCAGCAGGCGCTTCCGACTCCGTG
  ACCGTCGAAGAATCAGCAAAGATGTCCCTGGAGAGCAGTCAGGCTAAGCCTGTGAAGACTCCTGCTTCAA
  CCAAAAGGCTCAGCAAGACAGGGCTGAGCAAAGTCGATGTACGGGAGGACCCCAGCACCCTGGGGAAGAA
  AACGAAGTCACCCGGACGCGCACCTGGTACACCAGCACCTGTGCAAGAGGAGAACGATAGCACAGCCTTT
  ATGGAGACACCCAAGCAGAAGCTCGACTTCGCAGAAAACTCATCCGGATCAAAGCGACGGAGCAGAACCA
  GCAAGAATAGGAGCCAACCACTTGAAGACCTGGACGGGTTTCAGGAGCTGTTCCAGACACCCGCAGGAGC
  CTCCAATCCCGTATCCGTGGAAGAATCTGCTAAGATCTCACTTGAAAGCAGCCAAGCTGAGCCCGTACGC
  ACCAGGGCCTCCACAAAGCGACTTTCAAAGACCGGCTTGAACAAGATGGACGTCAGAGAGGGGCACTCTC
  CCCTGAGCAAATCCTCTTGCGCCAGTCAGAAGGTAATGCAGACTCTCACCCTGGGCGAGGATCATGGACG
  AGAAACTAAAGACGGAAAGGTCCTTCTTGCGCAGAAGCTGGAGCCAGCTATTTATGTCACGCGAGGCAAA
  CGACAGCAAAGGAGTTGCAAGAAGCGCAGCCAGTCACCAGAGGATTTGTCCGGTGTGCAGGAGGTTTTTC
  AGACATCTGGGCACAATAAAGATAGCGTCACTGTGGATAATTTGGCCAAGCTGCCCTCCTCTTCTCCACC
  TCTTGAACCTACCGATACTAGCGTGACCAGTAGGCGGCAAGCTCGCACCGGACTTCGGAAGGTCCATGTG
  AAGAACGAACTGAGTGGCGGGATAATGCACCCACAGATCAGCGGGGAGATAGTTGACCTGCCTCGGGAAC
  CGGAGGGCGAGGGTAAAGTGATTAAGACTCGGAAGCAGTCTGTGAAGCGCAAACTGGATACGGAGGTGAA
  CGTCCCACGCTCAAAGAGGCAGAGGATAACACGGGCTGAAAAGACGCTTGAAGACCTGCCAGGGTTCCAA
  GAACTGTGTCAGGCACCCTCTCTGGTAATGGACAGCGTAATCGTTGAAAAGACACCCAAGATGCCTGATA
  AGTCTCCCGAGCCCGTCGACACAACTTCTGAGACACAGGCGAGACGGAGACTGCGAAGACTTGTTGTCAC
  GGAAGAGCCTATCCCTCAGAGAAAAACAACACGAGTGGTCCGCCAAACTCGGAACACCCAGAAGGAGCCT
  ATCAGCGATAACCAAGGCATGGAAGAATTCAAGGAATCCTCCGTGCAGAAGCAGGACCCTTCCGTGAGCC
  TGACCGGTCGACGCAATCAACCCCGGACGGTCAAAGAAAAAACCCAGCCTCTGGAGGAGCTGACATCCTT
  CCAGGAGGAAACTGCAAAACGAATAAGTTCTAAAAGCCCCCAGCCCGAGGAGAAGGAGACACTTGCGGGA
  CTGAAGAGGCAGTTGCGGATTCAGTTGATCAACGACGGCGTGAAAGAGGAGCCTACTGCCCAGAGAAAGC
  AACCGAGCCGCGAGACTCGAAATACACTGAAAGAGCCTGTTGGCGACTCAATTAACGTGGAAGAGGTAAA
  GAAAAGCACCAAACAGAAGATCGATCCAGTTGCATCCGTGCCTGTGTCCAAGCGGCCCAGACGGGTGCCC
  AAGGAGAAGGCCCAGGCACTGGAGTTGGCCGGTCTGAAAGGCCCCATCCAGACCCTGGGACACACCGACG
  AGTCCGCGTCCGACAAAGGACCTACTCAAATGCCCTGTAACAGCTTGCAGCCCGAGCAGGTTGACAGCTT
  TCAAAGCAGTCCTAGACGGCCTAGGACAAGAAGGGGGAAAGTGGAAGCTGATGAAGAACCATCTGCTGTG
  AGAAAAACAGTCTCTACGTCCAGGCAGACCATGAGGAGCAGGAAGGTGCCCGAGATCGGGAATAATGGCA
  CACAGGTGTCAAAAGCCTCAATCAAGCAGACATTGGATACCGTTGCTAAGGTGACTGGGAGCAGACGACA
  GCTTAGGACACACAAGGACGGTGTACAACCACTCGAGGTCTTGGGAGACTCCAAGGAAATCACCCAGATT
  AGCGATCATTCCGAAAAGCTGGCCCACGATACGAGCATCCTGAAATCAACACAGCAGCAAAAGCCCGACT
  CTGTGAAACCGCTGCGGACCTGTCGGAGAGTGCTGCGGGCCTCCAAGGAAGACCCAAAGGAAGTGCTGGT
  GGACACACGGGACCACGCCACATTGCAGAGTAAGAGTAATCCCCTGCTCTCTCCCAAGAGGAAGTCCGCT
  CGCGACGGCTCTATCGTAAGAACCAGAGCACTGAGGTCCCTGGCCCCTAAACAGGAGGCCACTGATGAAA
  AGCCTGTCCCCGAAAAGAAGAGAGCAGCCTCCAGCAAAAGACATGTCAGTCCAGAGCCAGTGAAGATGAA
  GCACCTTAAGATCGTGAGCAATAAACTTGAGTCCGTGGAGGAGCAAGTGAGCACAGTTATGAAGACCGAG
  GAGATGGAGGCTAAACGCGAGAACCCTGTCACACCTGATCAGAACAGTCGGTACCGAAAGAAGACTAACG
  TGAAGCAGCCTCGGCCGAAGTTCGATGCGTCCGCGGAAAATGTGGGCATAAAGAAAAACGAGAAGACGAT
  GAAAACGGCTTCCCAGGAAACAGAGCTTCAGAATCCGGATGACGGTGCCAAAAAGTCTACGTCCAGAGGG
  CAGGTTTCAGGAAAGCGGACTTGTCTCAGATCAAGGGGGACAACCGAGATGCCACAGCCTTGCGAAGCAG
  AGGAAAAAACCAGTAAGCCTGCCGCCGAAATCCTGATTAAACCCCAGGAAGAGAAGGGAGTCAGTGGAGA
  ATCAGATGTCAGGTGCTTGAGGTCTAGAAAGACCAGGGTCGCCCTGGACAGCGAGCCAAAGCCCCGAGTG
  ACTAGAGGTACCAAAAAGGATGCCAAGACGCTGAAAGAGGATGAGGATATCGTCTGCACCAAGAAGCTGC
  GGACCAGGTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227041 representing NM_001081117
  Red=Cloning site Green=Tags(s)
  
  MASSAHLVTIKRSGDDGAHFPLSLSSCLFGRSIECDIRIQLPVVSKRHCKIEVKEQEAILYNFSSTNPTQ
  VNGVTIDEPVRLRHGDIITIIDRSFRYEDGNHEDGSKPTEFPGKSLGKEPSRRASRDSFCADPDGEGQDT
  KASKMTASRRSFVYAKGLSADSPASDGSKNSVSQDSSGHVEQHTGRNIVEPTSGDLFKKSRSTGSSYREP
  KSSPTQSLSNSNEKESPFEKLYQSMKEELDVKSQKSCRKSEPQPDRAAEESRETQLLVSGRARAKSSGST
  PVTAASSPKVGKIWTERWRGGMVPVQTSTETAKMKTPVRHSQQLKDEDSRVTGRRHSVNLDEGESAQAVH
  KTVTPGKLATRNQAAVEAGDVASPADTPEHSSSKKRSIPAKVEAPSAETQKRLSLTQRLVPGEKKTPKGS
  FSKPEKLATAAEQTCSGLPGLSSVDISNFGDSINKSEGMPMKRRRVSFGGHLRPELFDENLPPNTPLKRG
  ETPTKRKSLGTHSPAVLKTIIKERPQSPGKQESPGITPPRTNDQRRRSGRTSSGSKFLCETDIPKKAGRK
  SGNLPAKRASISRSQHGILQMICSKRRSGASEANLIVAKSWADVVKLGVKQTQTKVAKHVPQKQTSKRQR
  RPSTPKKPTSNLHNQFTTGHANSPCTIVVGRAQIEKVSVPARPYKMLNNLMLNRKVDFSEDLSGLTEMFK
  TPVKEKQQQMSDTGSVLSNSANLSERQLQVTNSGDIPEPITTEILGEKVLSSTRNAAKQQSDRYSASPTL
  RRRSIKHENTVQTPKNVHNITDLEKKTPVSETEPLKTASSVSKLRRSRELRHTLVETMNEKTEAVLAENT
  TARHLRGTFREQKVDQQVQDNENAPQRCKESGELSEGSEKTSARRSSARKQKPTKDLLGSQMVTQTADYA
  EELLSQGQGTIQNLEESMHMQNTSISEDQGITEKKVNIIVYATKEKHSPKTPGKKAQPLEGPAGLKEHFE
  TPNPKDKPITEDRTRVLCKSPQVTTENITTNTKPQTSTSGKKVDMKEESSALTKRIHMPGESRHNPKILK
  LECEDIKALKQSENEMLTSTVNGSKRTLEKSKKKAQPLEDLTCFQELFISPVPTNIIKKIPSKSPHTQPV
  RTPASTKRLSKTGLSKVDVRQEPSTLGKRTKSPGRAPGTPAPVQEENDSTAFMETPKQKLDFAGNSSGSK
  RRSRTSKNRSQPLEDLDGFQELFQTPAGASDSVTVEESAKISLESSQAEPVKTPASTKRRSKMSLMKVDM
  KELSILEKQTQSRGRDAGTPAPMQEGNGTTAIMETPKQKLDFTGNSTGHKRRPRTPKIRAQPLEDLDGFQ
  ELFQTPAGANDSVTVEESAKMSLESSQAEPVKTPASTKRLSKTDLSKVDVREDPSILGKKTKSPGRAPGT
  PAPVQEENDCTAYMETPKQKLESIENLTGLRKQSRTPKDITGFQDSFQIPDHANGPLVVVKTKKMFFNSP
  QPESAITRKSRERQSRASISKIDVKEELLESEEHLQLGEGVDTFQVSTNKVIRSSRKPAKRKLDSTAGMP
  NSKRMRCSSKDNTPCLEDLNGFQELFQMPGYANDSLTTGISTMLARSPQLGPVRTQINKKSLPKIILRKM
  DVTEEISGLWKQSLGRVHTTQEQEDNAIKAIMEIPKETLQTAADGTRLTRQPQTPKEKVQPLEDHSVFQE
  LFQTSRYCSDPLIGNKQTRMSLRSPQPGFVRTPRTSKRLAKTSVGNIAVREKISPVSLPQCATGEVVHIP
  IGPEDDTENKGVKESTPQTLDSSASRTVSKRQQGAHEERPQFSGDLFHPQELFQTPASGKDPVTVDETTK
  IALQSPQPGHIINPASMKRQSNMSLRKDMREFSILEKQTQSRGRDAGTPAPMQEENGTTAIMETPKQKLD
  FIGNSTGHKRRPRTPKNRAQPLEDLDGFQELFQTPAGASDPVSVEESAKISLASSQAEPVRTPASTKRRS
  KTGLSKVDVRQEPSTLGKRMKSLGRAPGTPAPVQEENDSTAFMETPKQKLDFTGNSSGHKRRPQTPKIRA
  QPLEDLDGFQELFQTPAGANDSVTVEESVKMSLESSQAEPVKTPASTKRLSKTGLSKVDVREDPSILEKK
  TKSPGTPAPVQEENDCTAFMETPKQKLDFTGNSSGHKRRPRTPKIRAQPLEDLDGFQELFQTPAGASDSV
  TVEESAKMSLESSQAKPVKTPASTKRLSKTGLSKVDVREDPSTLGKKTKSPGRAPGTPAPVQEENDSTAF
  METPKQKLDFAENSSGSKRRSRTSKNRSQPLEDLDGFQELFQTPAGASNPVSVEESAKISLESSQAEPVR
  TRASTKRLSKTGLNKMDVREGHSPLSKSSCASQKVMQTLTLGEDHGRETKDGKVLLAQKLEPAIYVTRGK
  RQQRSCKKRSQSPEDLSGVQEVFQTSGHNKDSVTVDNLAKLPSSSPPLEPTDTSVTSRRQARTGLRKVHV
  KNELSGGIMHPQISGEIVDLPREPEGEGKVIKTRKQSVKRKLDTEVNVPRSKRQRITRAEKTLEDLPGFQ
  ELCQAPSLVMDSVIVEKTPKMPDKSPEPVDTTSETQARRRLRRLVVTEEPIPQRKTTRVVRQTRNTQKEP
  ISDNQGMEEFKESSVQKQDPSVSLTGRRNQPRTVKEKTQPLEELTSFQEETAKRISSKSPQPEEKETLAG
  LKRQLRIQLINDGVKEEPTAQRKQPSRETRNTLKEPVGDSINVEEVKKSTKQKIDPVASVPVSKRPRRVP
  KEKAQALELAGLKGPIQTLGHTDESASDKGPTQMPCNSLQPEQVDSFQSSPRRPRTRRGKVEADEEPSAV
  RKTVSTSRQTMRSRKVPEIGNNGTQVSKASIKQTLDTVAKVTGSRRQLRTHKDGVQPLEVLGDSKEITQI
  SDHSEKLAHDTSILKSTQQQKPDSVKPLRTCRRVLRASKEDPKEVLVDTRDHATLQSKSNPLLSPKRKSA
  RDGSIVRTRALRSLAPKQEATDEKPVPEKKRAASSKRHVSPEPVKMKHLKIVSNKLESVEEQVSTVMKTE
  EMEAKRENPVTPDQNSRYRKKTNVKQPRPKFDASAENVGIKKNEKTMKTASQETELQNPDDGAKKSTSRG
  QVSGKRTCLRSRGTTEMPQPCEAEEKTSKPAAEILIKPQEEKGVSGESDVRCLRSRKTRVALDSEPKPRV
  TRGTKKDAKTLKEDEDIVCTKKLRTRS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081117
• ORF Size: 9531 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081117.2, NP_001074586.2
• RefSeq Size: 10098 bp
• RefSeq ORF: 9534 bp
• Locus ID: 17345
• UniProt ID: E9PVX6
• Cytogenetics: 7 F3
• Gene Summary: Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (By similarity). Does not contribute to the internal structure of mitotic chromosomes (PubMed:26949251). May play a role in chromatin organization (PubMed:26949251). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed.[UniProtKB/Swiss-Prot Function]