Mki67 (NM_001081117) Mouse Untagged Clone

CAT#: MC225467

Mki67 (untagged) - Mouse antigen identified by monoclonal antibody Ki 67 (Mki67), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mki67
• Synonyms: D630048A14Rik; Ki-67; Ki67
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225467 representing NM_001081117
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCCTCGGCTCACCTGGTCACCATCAAGCGGAGCGGCGATGACGGCGCACACTTCCCGCTGAGCC
  TCAGCTCCTGCCTGTTTGGAAGGAGTATTGAATGTGACATTCGTATCCAGCTGCCTGTAGTGTCAAAAAG
  ACATTGCAAAATTGAAGTCAAAGAGCAAGAGGCAATATTATATAATTTCAGTTCTACCAATCCAACTCAA
  GTAAACGGGGTTACTATAGATGAGCCTGTGAGGCTGAGACATGGAGACATAATAACCATCATTGACCGCT
  CCTTTAGGTATGAAGATGGAAATCATGAGGATGGAAGCAAGCCAACAGAATTTCCAGGAAAGTCCCTTGG
  AAAGGAACCATCAAGGCGAGCCTCAAGAGATAGCTTCTGTGCTGACCCTGATGGGGAAGGTCAAGATACC
  AAAGCTTCAAAAATGACTGCTTCAAGAAGATCTTTTGTGTATGCCAAGGGCCTTTCTGCAGATAGCCCTG
  CCTCAGATGGCTCAAAGAACAGTGTTAGCCAAGACTCATCAGGGCATGTAGAACAGCACACTGGCAGAAA
  CATAGTAGAGCCCACTTCTGGGGATCTCTTTAAGAAGTCCAGGTCTACAGGGAGCAGTTACAGGGAACCG
  AAGTCTTCTCCTACACAGAGCCTTAGCAATAGCAACGAAAAGGAATCTCCCTTTGAGAAACTTTATCAAT
  CAATGAAGGAAGAGTTGGATGTAAAATCCCAGAAATCTTGTAGGAAATCAGAACCCCAACCTGACCGTGC
  AGCAGAGGAATCGCGGGAGACACAGCTATTGGTGTCAGGCAGGGCAAGAGCAAAGTCTAGTGGAAGCACC
  CCTGTTACTGCAGCCTCTTCACCCAAAGTAGGAAAGATCTGGACTGAGAGATGGCGCGGTGGAATGGTGC
  CTGTCCAGACTTCCACAGAGACAGCTAAAATGAAGACCCCTGTGCGGCATTCACAGCAACTTAAGGATGA
  AGACTCTCGTGTTACTGGCAGACGACATTCTGTGAATCTGGATGAAGGTGAAAGTGCCCAGGCAGTCCAT
  AAAACAGTCACTCCTGGGAAACTGGCGACTAGAAACCAAGCTGCGGTGGAGGCTGGGGATGTTGCCAGCC
  CCGCTGATACACCAGAACATTCCTCTTCCAAAAAGAGAAGTATTCCTGCAAAGGTAGAGGCTCCATCTGC
  AGAGACACAAAAGCGGCTCTCTTTAACTCAGCGCCTTGTTCCAGGTGAAAAGAAAACTCCCAAGGGTTCC
  TTCAGCAAGCCTGAGAAACTGGCCACAGCCGCCGAACAGACTTGCTCTGGCCTACCTGGTCTTAGTTCCG
  TTGATATCAGCAACTTTGGTGATTCCATTAACAAGAGTGAGGGAATGCCTATGAAGAGAAGACGTGTATC
  CTTTGGTGGACATCTAAGACCTGAATTATTTGATGAAAACTTGCCTCCTAATACACCACTGAAAAGAGGA
  GAAACGCCAACCAAGAGGAAGTCTCTTGGCACTCACAGCCCAGCTGTCCTCAAGACAATCATCAAGGAAC
  GGCCCCAGTCTCCAGGGAAACAAGAGTCTCCTGGGATAACGCCACCGAGGACAAATGATCAAAGACGCAG
  ATCAGGCAGGACTTCCAGTGGAAGCAAATTCTTATGTGAGACAGACATTCCCAAGAAAGCAGGCAGGAAG
  AGCGGTAACCTGCCTGCGAAGAGAGCATCCATCAGCCGGAGTCAGCATGGCATTCTACAGATGATTTGCT
  CCAAAAGGCGAAGTGGAGCTTCTGAAGCCAACTTGATTGTTGCAAAATCATGGGCTGATGTTGTAAAACT
  TGGCGTGAAACAAACACAAACGAAAGTTGCGAAACATGTCCCTCAAAAGCAGACGAGCAAGAGACAAAGA
  AGACCCAGCACTCCAAAGAAACCCACAAGCAATCTTCACAATCAATTTACTACAGGCCATGCAAACTCTC
  CCTGTACCATTGTAGTAGGTAGAGCGCAGATTGAAAAAGTAAGTGTGCCTGCCCGACCCTACAAAATGCT
  GAATAACTTGATGCTAAACCGAAAAGTGGACTTCAGTGAAGATCTGTCAGGACTAACTGAAATGTTCAAG
  ACTCCAGTGAAGGAGAAGCAGCAGCAGATGAGTGATACAGGCTCCGTACTTTCCAATTCAGCGAATTTGT
  CTGAAAGACAATTGCAAGTAACTAATTCAGGAGACATACCTGAGCCCATCACCACAGAGATTTTGGGAGA
  AAAAGTGCTATCCAGTACTCGGAATGCAGCAAAGCAGCAGTCTGATAGATATTCTGCAAGTCCTACCTTA
  AGACGGCGGAGCATCAAACATGAAAACACAGTGCAAACTCCTAAGAATGTCCATAACATTACTGACCTTG
  AGAAGAAGACTCCGGTCTCTGAGACAGAGCCCCTGAAGACTGCATCGAGTGTGAGCAAGTTAAGAAGATC
  TAGAGAGCTCAGACATACCCTTGTGGAAACTATGAATGAAAAAACAGAAGCAGTCCTTGCTGAGAACACC
  ACAGCAAGACATTTAAGGGGGACATTTCGAGAACAAAAAGTAGATCAACAGGTGCAGGACAATGAAAACG
  CTCCTCAAAGATGCAAGGAAAGTGGTGAATTAAGTGAAGGTTCAGAAAAGACATCAGCTAGGAGATCAAG
  TGCCAGGAAGCAGAAGCCGACAAAAGACTTACTAGGAAGTCAGATGGTCACCCAAACAGCAGACTATGCT
  GAGGAACTACTTAGTCAAGGACAAGGAACCATACAAAACCTAGAGGAATCCATGCACATGCAAAACACAT
  CAATAAGTGAGGATCAAGGAATTACAGAAAAGAAAGTGAACATAATAGTATATGCAACCAAAGAGAAGCA
  CTCGCCAAAGACCCCTGGCAAAAAGGCACAACCTCTAGAAGGGCCAGCTGGTCTCAAGGAACACTTTGAA
  ACACCAAACCCCAAAGATAAACCTATAACGGAAGACAGAACTAGAGTCCTTTGCAAATCACCACAAGTCA
  CAACAGAGAATATCACAACAAACACAAAGCCACAGACTAGCACATCTGGGAAGAAAGTAGACATGAAGGA
  AGAAAGCTCTGCCTTGACAAAACGTATACATATGCCAGGGGAATCCAGGCATAATCCCAAAATTTTAAAA
  CTTGAGTGTGAGGATATCAAAGCTTTGAAGCAATCTGAAAATGAAATGCTGACCTCAACAGTAAATGGAA
  GCAAGAGGACTTTAGAAAAATCTAAAAAAAAGGCTCAGCCCCTGGAAGACCTGACTTGTTTCCAGGAACT
  CTTTATATCACCAGTTCCTACTAACATAATCAAAAAAATTCCCAGCAAATCTCCACACACACAACCAGTC
  AGAACCCCAGCGAGCACAAAGAGACTCTCCAAGACAGGTCTCAGTAAAGTGGATGTGAGACAAGAACCTT
  CAACACTTGGGAAAAGAACGAAGTCACCAGGCAGAGCCCCAGGCACACCAGCACCAGTGCAGGAAGAAAA
  TGACAGCACAGCCTTCATGGAAACTCCAAAGCAGAAACTGGATTTTGCAGGGAATTCATCAGGGAGTAAG
  AGAAGGTCACGAACATCTAAGAACAGGTCTCAGCCCCTAGAAGACCTGGATGGCTTCCAAGAACTCTTCC
  AAACACCAGCTGGTGCCAGTGACTCAGTGACTGTTGAGGAAAGTGCAAAGATATCTTTGGAATCTTCACA
  AGCAGAACCAGTCAAAACCCCGGCAAGCACAAAGAGACGGTCTAAGATGAGTCTCATGAAAGTGGATATG
  AAAGAACTTTCCATACTTGAGAAACAAACACAGTCACGAGGCAGAGACGCAGGCACACCAGCACCAATGC
  AGGAAGGAAATGGCACCACAGCCATTATGGAAACTCCAAAGCAGAAACTGGATTTCACAGGAAATTCAAC
  AGGACATAAGAGGAGGCCACGGACACCTAAGATCAGAGCTCAGCCCCTAGAAGACCTGGATGGCTTCCAA
  GAACTCTTCCAAACACCAGCTGGTGCTAATGACTCAGTGACTGTTGAGGAAAGTGCAAAGATGTCTTTGG
  AATCTTCACAAGCAGAACCAGTCAAAACCCCGGCAAGCACAAAGAGACTCTCCAAGACAGATCTCAGTAA
  GGTGGATGTGAGAGAAGACCCTTCAATACTTGGGAAAAAAACAAAGTCACCAGGCAGAGCCCCAGGTACA
  CCAGCACCAGTGCAGGAAGAAAATGACTGCACAGCCTACATGGAAACTCCAAAGCAGAAACTGGAGTCTA
  TAGAAAATTTAACAGGGCTTAGGAAACAGTCCAGAACACCTAAAGACATCACTGGTTTCCAGGATAGTTT
  CCAAATACCAGATCATGCTAATGGCCCATTAGTGGTTGTCAAAACCAAAAAAATGTTCTTTAATTCTCCA
  CAACCAGAAAGTGCCATAACCCGAAAGAGCAGAGAGAGACAGTCTAGGGCAAGTATAAGTAAAATAGATG
  TTAAAGAAGAACTTTTAGAATCAGAGGAACACCTACAATTAGGAGAAGGTGTAGACACATTTCAGGTATC
  CACCAACAAAGTCATTAGATCATCTAGGAAACCTGCAAAGCGTAAACTGGATTCAACAGCTGGTATGCCT
  AACAGCAAGAGGATGCGCTGTTCTTCAAAGGATAACACACCATGCCTAGAAGACCTGAATGGCTTCCAAG
  AGCTCTTCCAAATGCCAGGCTATGCTAATGACTCTTTGACCACTGGAATCTCAACAATGCTTGCTAGATC
  ACCACAATTAGGACCAGTTAGAACCCAAATCAACAAAAAGAGTCTGCCCAAGATCATCTTGAGAAAAATG
  GATGTGACAGAAGAAATTTCAGGTCTCTGGAAGCAGTCACTGGGCAGAGTCCACACCACACAAGAGCAGG
  AGGATAATGCAATCAAAGCAATTATGGAGATTCCAAAGGAAACACTGCAGACTGCAGCAGATGGAACTAG
  GCTTACCAGACAGCCACAAACACCTAAGGAAAAAGTTCAACCGCTGGAAGATCACAGTGTCTTCCAAGAA
  CTCTTCCAAACATCACGCTACTGTTCTGATCCATTAATTGGTAACAAACAAACAAGAATGTCCTTGAGAT
  CTCCACAACCAGGATTTGTTAGAACTCCACGAACCTCAAAGAGACTGGCTAAGACAAGTGTTGGGAATAT
  TGCTGTGAGAGAAAAGATCTCTCCAGTGAGTCTGCCACAGTGTGCTACAGGGGAGGTTGTACACATACCC
  ATAGGGCCAGAAGATGACACAGAGAACAAAGGTGTGAAGGAATCCACACCTCAGACACTGGACTCATCAG
  CAAGTCGAACTGTCAGCAAGAGGCAGCAAGGGGCACATGAGGAAAGGCCTCAGTTCTCAGGAGACTTATT
  TCATCCCCAAGAGCTCTTTCAAACACCAGCCAGTGGCAAAGACCCAGTAACTGTTGATGAAACTACAAAA
  ATAGCTCTGCAGTCTCCACAACCAGGACATATCATAAACCCAGCAAGCATGAAGAGACAGTCCAACATGA
  GTCTCAGGAAAGACATGAGAGAATTTTCCATACTTGAAAAACAAACACAGTCACGAGGCAGAGACGCAGG
  CACACCAGCACCAATGCAGGAAGAAAATGGCACCACAGCCATTATGGAAACACCAAAGCAGAAACTGGAT
  TTCATAGGAAATTCAACAGGACATAAGAGGAGGCCTCGGACACCCAAAAACAGGGCTCAGCCCCTAGAAG
  ACCTGGATGGCTTCCAAGAACTCTTTCAAACACCAGCTGGTGCCAGTGACCCTGTGAGTGTTGAAGAAAG
  TGCAAAGATATCTTTGGCATCTTCACAAGCAGAACCAGTCAGAACCCCAGCAAGTACAAAGAGACGCTCC
  AAGACAGGTCTCAGTAAAGTGGATGTGAGACAAGAACCTTCAACACTTGGGAAAAGAATGAAGTCACTAG
  GCAGAGCCCCAGGCACACCAGCACCAGTGCAGGAAGAAAATGACAGCACAGCCTTCATGGAAACTCCAAA
  GCAGAAACTGGATTTCACAGGAAATTCATCAGGACATAAGAGGAGGCCACAGACACCTAAGATCAGGGCT
  CAGCCCCTAGAAGACCTGGATGGCTTCCAAGAACTCTTCCAAACACCAGCTGGTGCCAATGACTCAGTGA
  CTGTTGAGGAAAGTGTAAAGATGTCTTTGGAATCTTCACAAGCAGAACCAGTCAAAACCCCGGCAAGCAC
  AAAGAGACTCTCCAAGACAGGTCTCAGTAAGGTGGATGTGAGAGAAGACCCTTCAATACTTGAGAAAAAA
  ACAAAGTCACCAGGCACACCAGCACCAGTGCAGGAAGAAAATGACTGCACAGCCTTCATGGAAACTCCAA
  AGCAGAAACTGGATTTCACAGGAAATTCATCAGGACATAAGAGGAGGCCACGGACACCTAAGATCAGAGC
  TCAGCCCCTAGAAGACCTGGATGGCTTCCAAGAACTCTTCCAAACACCAGCTGGTGCTAGTGACTCAGTG
  ACTGTTGAGGAAAGTGCAAAGATGTCTTTGGAATCTTCACAAGCAAAACCAGTCAAAACCCCGGCAAGCA
  CAAAGAGACTCTCCAAGACAGGTCTCAGTAAGGTGGATGTGAGAGAAGACCCTTCAACACTTGGGAAAAA
  AACAAAGTCACCAGGCAGAGCCCCAGGCACACCAGCACCAGTGCAGGAAGAAAATGACAGCACAGCCTTC
  ATGGAAACTCCAAAGCAGAAACTGGATTTTGCAGAGAATTCATCAGGGAGTAAGAGAAGGTCACGAACAT
  CTAAGAACAGGTCTCAGCCCCTAGAAGACCTGGATGGCTTCCAAGAACTCTTCCAAACACCAGCTGGTGC
  CAGTAACCCTGTGAGTGTTGAAGAAAGTGCAAAGATATCTTTGGAATCTTCACAAGCAGAACCAGTCAGA
  ACCCGGGCAAGCACAAAGAGACTTTCCAAGACAGGTCTCAATAAGATGGATGTGAGAGAAGGGCACTCTC
  CGCTCAGTAAGTCAAGCTGTGCATCACAGAAAGTCATGCAAACCCTCACACTTGGAGAAGATCATGGCAG
  AGAGACCAAAGATGGGAAGGTATTGTTAGCTCAGAAATTGGAACCAGCAATATATGTTACTCGTGGCAAG
  AGGCAGCAAAGGTCATGTAAGAAAAGGTCCCAGTCCCCAGAAGACCTCTCTGGTGTTCAGGAGGTCTTCC
  AAACATCAGGCCATAACAAGGATTCAGTGACAGTGGACAATCTTGCAAAACTGCCCAGCTCGTCTCCACC
  ACTAGAGCCAACAGACACTTCAGTAACCTCACGGAGACAGGCCAGAACTGGTCTGAGGAAAGTTCACGTG
  AAAAATGAACTTTCAGGAGGCATAATGCATCCACAAATATCAGGGGAAATTGTGGACTTACCTAGAGAAC
  CAGAAGGTGAAGGCAAAGTCATTAAAACAAGGAAGCAATCTGTAAAACGGAAATTGGACACAGAAGTCAA
  TGTGCCTCGCAGTAAGAGGCAAAGAATTACAAGAGCAGAAAAGACCCTAGAGGATCTGCCTGGCTTCCAA
  GAGCTCTGCCAAGCTCCAAGCTTGGTAATGGACTCAGTTATTGTTGAGAAAACCCCAAAGATGCCCGACA
  AATCTCCAGAACCTGTGGATACAACTTCAGAGACACAGGCAAGAAGAAGACTCAGGAGACTGGTTGTTAC
  TGAAGAGCCCATACCACAAAGAAAGACTACAAGAGTTGTAAGGCAAACCAGAAACACACAGAAAGAGCCC
  ATAAGTGACAATCAAGGTATGGAAGAGTTTAAGGAATCTTCAGTACAGAAACAAGACCCAAGTGTAAGTT
  TAACTGGCAGGAGGAACCAACCAAGGACAGTTAAGGAGAAAACCCAACCCTTAGAAGAACTCACCAGTTT
  CCAAGAGGAAACTGCCAAAAGAATATCTTCCAAATCTCCACAACCGGAAGAGAAGGAAACCTTAGCAGGT
  TTAAAGAGGCAGCTCAGAATACAACTAATCAACGATGGTGTAAAAGAAGAGCCCACAGCACAGAGAAAGC
  AACCATCCAGGGAAACCAGGAACACACTCAAAGAGCCTGTAGGTGACAGTATAAATGTTGAAGAGGTTAA
  GAAGTCTACAAAGCAGAAAATTGATCCAGTAGCAAGTGTGCCTGTCAGCAAGAGGCCACGGAGGGTACCC
  AAGGAAAAGGCACAGGCCCTAGAATTGGCTGGTCTCAAAGGACCAATCCAAACCCTAGGCCACACTGATG
  AATCAGCAAGTGATAAAGGACCCACACAGATGCCCTGTAATTCTCTACAACCAGAGCAAGTTGACAGCTT
  CCAAAGCTCACCAAGGCGACCCAGGACAAGACGTGGGAAAGTAGAGGCAGATGAAGAGCCTTCAGCAGTA
  AGAAAGACAGTATCAACATCAAGGCAAACTATGCGATCCCGCAAGGTCCCTGAAATTGGTAACAATGGTA
  CCCAAGTTTCAAAGGCCTCCATAAAGCAGACATTAGATACAGTAGCCAAAGTAACTGGCAGCAGGAGGCA
  GCTAAGGACACATAAGGATGGGGTTCAACCTCTTGAAGTGTTAGGTGACTCCAAAGAAATAACCCAAATA
  TCAGATCACTCTGAGAAACTAGCACATGACACCAGTATCCTTAAGAGCACTCAACAGCAAAAGCCAGACT
  CAGTAAAACCTCTGAGAACATGCAGAAGAGTGCTGAGGGCCTCTAAAGAGGACCCCAAGGAAGTGTTGGT
  GGACACCAGAGACCATGCAACATTACAAAGCAAAAGCAACCCTTTGCTGTCCCCGAAGAGGAAGTCTGCA
  AGAGATGGAAGCATTGTGAGAACCAGGGCTTTGCGCTCTTTAGCACCAAAGCAGGAAGCAACAGATGAGA
  AGCCTGTACCTGAGAAAAAAAGGGCTGCTTCCAGCAAGAGGCATGTATCACCTGAGCCTGTGAAGATGAA
  ACACCTGAAAATCGTGTCAAACAAACTTGAATCTGTGGAAGAGCAGGTTAGCACTGTTATGAAAACAGAA
  GAAATGGAAGCCAAAAGAGAAAATCCTGTCACTCCAGATCAGAACTCTAGGTACCGAAAGAAAACCAATG
  TAAAACAGCCAAGGCCCAAGTTTGATGCATCTGCAGAGAATGTCGGGATAAAGAAAAACGAGAAGACTAT
  GAAGACTGCCTCCCAGGAGACAGAGCTGCAGAATCCAGATGATGGAGCCAAGAAATCTACATCTCGGGGC
  CAAGTCAGTGGGAAAAGAACATGCTTGAGGTCTAGAGGAACGACTGAGATGCCCCAGCCTTGTGAAGCAG
  AAGAGAAAACAAGCAAACCAGCTGCAGAAATCTTGATAAAGCCTCAGGAAGAGAAAGGAGTCTCTGGAGA
  GTCTGATGTTAGGTGTTTGAGGTCCAGAAAAACTAGAGTCGCTTTGGACAGTGAACCTAAGCCAAGGGTA
  ACTCGTGGAACCAAGAAAGATGCAAAAACTCTGAAGGAGGATGAAGACATTGTATGCACCAAGAAGTTAA
  GAACAAGAAGTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081117
• Insert Size: 9534 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081117.2, NP_001074586.2
• RefSeq Size: 10098 bp
• RefSeq ORF: 9534 bp
• Locus ID: 17345
• UniProt ID: E9PVX6
• Cytogenetics: 7 F3
• Gene Summary: Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (By similarity). Does not contribute to the internal structure of mitotic chromosomes (PubMed:26949251). May play a role in chromatin organization (PubMed:26949251). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed.[UniProtKB/Swiss-Prot Function]