Nuggc (NM_001195674) Mouse Tagged ORF Clone

CAT#: MG226953

Nuggc (tGFP-tagged) - Mouse predicted gene 600 (Gm600), (10ug)

Product Images

Circular map for MG226953

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nuggc
• Synonyms: Gm600; SLIP-GC
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226953 representing NM_001195674
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTTGGACTCTATGAAATCCAAGGAGGAGCTCGGAGTAGCACCTCTTGGGACCGGCTATTCCAGTT
  CATTCCAAGGCGTTTCCCCTGCTGACTGCGATCTGTTTAAAGAACCTGTTAGGAAAAGACGCAGGTTGCA
  TCGAGACCGGCAATTCCAGGCCTTTCCTTCTGCAGAGCAAAGTGCTCTTAAGGAATATGAAAAACTGGAG
  TGTCGGACCAGGAGGGTCTTGAGCAACACTTATCAGAAATTGATTCAGTCTGTCTTCCTGGATGACAGCA
  TTCCCAGCGGACTCAAGTATCTCATAAACCGGCTTCTTGCCTTGATTGAAAAATCCCCACTGGAGCCAGT
  CTATGTGGGGTTCTTGGGAATCACTGGAGCTGGCAAGAGCTCTCTGATCAATGCCCTCATCCGGCAAGCA
  ATGTTTCTGCCTGTGTCTGGAGAAAGTGTTTGCACATCATGCATCGTACAAGTGAGCTCTGGCTGCTGTG
  AGCAGTATGAGGCCAAAATTCACCTTCTGTCCGATCAGGAGTGGAAGGCAGAACTGAAGGACTTGACTAA
  GCTCCTGCACAGAGCAGAGCAGTCTGGGGAAGAGGAGGCAGACTTGTGGGACAGGGATGATGCAACGGAA
  GAAGCTGCCCAGAAGCTACGGATGCTCTATGGCCATGGGGCAGAAAGGAGGCACTACGAGGAGTTGCTAA
  GGCTGAAGCCCAGAGGGAGGATTCCCAACTCGAGAACCATCACTCTAAAGGCAGAGGAGGCAGGAGAGCT
  ATCTGTCAAACTGGACCCCTACATCAGGACCAGGAGGAGAGACTGGGATGGAGAATCAGCAGAGACACAA
  ATCTGGCCCTTGATCAAATATGTGGAAGTGATTCTTCCCAAATCTGCACTGATCCCAGAAGGGGTTGTCC
  TGGTAGACATTCCAGGCACAGGAGACTTCAACAGCAAGAGGGATAAAATGTGGAAAAAGACCATTGACAA
  GTGTTCCGTGATCTGGGTGATCAGCGACATCGAGCGAGTTTCTGGAGGAAAGACCCATGAAGACCTTCTG
  AGCGAGAGCATCAAAGCCTGCCAGCGTGGCTTCTGCAGGGACATCGCCCTGGTGGTCACCAAAACAGACA
  AACTCCACTTGCAGGAATATCTCAGGGAAAGAAAGATGGGAAATCAAGCTATTCAGAGTCAGAGAGAAGC
  TGTTCTGCAAAGAAATGAAATAATCAAACTACAAAGGAAGAGAATGCTCAAGGAGAAATTGAAGAGAAAA
  CTGCCAGCTGACTCCAAGGTTCTGGAAGCCTCAGATCTGGTGTACACGGTCAGTGCCCATGAGTACTGGC
  AGCGGACCATCCTGACTGAGGAAGAAAGTGAGATCCCCAAGTTAAGAGAATATATTAGGAAAAGGATCTT
  GGACAAGAAGAGGAGGCTGGTGACCAAGTACGTCACAGAAGCCTTTGGCCTATTGCTTCTCACAGACACG
  TTAAACACGGAGGAAAGCCTGCTGACTGAAGAACTGAACACGGGTGGCCTGCGACAATTTGTAGAGGAGA
  AGATGGAGCTGTTGGAGAAGGCCATCGAGCAGTGCTTTGCTCGAATGGAACAGCCCCTGCAGACAGGGGT
  CCAGGTGGCCATGACTTCCTACCGCCGCATCCTCGGGTCATGCCTGGTGAGGAGCAGAGGAAACCAAGGT
  TTTCACCAGACCCTGAAAGCAGTTTGCCTGAAAAATGGGGTCTATGCCTCCAGAACTCTGGCCAGAATTG
  ACCTGAATGAAGCCCTGTCTCAGCCTATCTATGACCAGATTGACCCTGTATTTGGAGGCATTTTTAGGGA
  TGGGAAGCCCACTGCCCCTGCTCTGATGCAGCACATAGATGCTTTCAAGCATTCCCTGGAGGAGAGGATG
  GCAGAAGTTGGGGTTAGAAGTGGCTGGAAACAAGATGGATACAAGAGAAGCTTCCTGATCCAGGAGATAA
  GCGCCATCCTGGGAGGCCTGGAGAGCCACATCCTCCGGAGGAAAAGGAAGATCTATAAGTCTGTCACCAG
  CTCCATCCAAAACGACCTGAAGCCCTGCTATGAAGAGGCAGCTCAGATCACTGGCAAGAAAGCATGCGAG
  AGAATGAAGGATGTCATCAGGAGAGGGGTGGAGCGGCAGGTGGCTGAGGGCTTGTTTGAGAGGGCTCAAG
  AGAGGATGTGGCACCAGTTTCGACAGCTGAAGCATGGAATCACTGAGAAGGTCAAGGGCAGCATCACTAC
  CATGTTAACCCTTGCTGCACCCCAGGGAGTTGGACTCTGCAAGGAGCTTGCAGATGTCCGGAATGAACAG
  AAGGAAATGGAAAAACTCTACAGAAGCCTGAGGGAGGTTGCTGAGAACGCACAGCTGCGGAGGAGCATGC
  AAGATTTCCTCCTGCGAATGTCCCCCAGCAAAGCCGGCCCCCATGGAACAAAACTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226953 representing NM_001195674
  Red=Cloning site Green=Tags(s)
  
  MDLDSMKSKEELGVAPLGTGYSSSFQGVSPADCDLFKEPVRKRRRLHRDRQFQAFPSAEQSALKEYEKLE
  CRTRRVLSNTYQKLIQSVFLDDSIPSGLKYLINRLLALIEKSPLEPVYVGFLGITGAGKSSLINALIRQA
  MFLPVSGESVCTSCIVQVSSGCCEQYEAKIHLLSDQEWKAELKDLTKLLHRAEQSGEEEADLWDRDDATE
  EAAQKLRMLYGHGAERRHYEELLRLKPRGRIPNSRTITLKAEEAGELSVKLDPYIRTRRRDWDGESAETQ
  IWPLIKYVEVILPKSALIPEGVVLVDIPGTGDFNSKRDKMWKKTIDKCSVIWVISDIERVSGGKTHEDLL
  SESIKACQRGFCRDIALVVTKTDKLHLQEYLRERKMGNQAIQSQREAVLQRNEIIKLQRKRMLKEKLKRK
  LPADSKVLEASDLVYTVSAHEYWQRTILTEEESEIPKLREYIRKRILDKKRRLVTKYVTEAFGLLLLTDT
  LNTEESLLTEELNTGGLRQFVEEKMELLEKAIEQCFARMEQPLQTGVQVAMTSYRRILGSCLVRSRGNQG
  FHQTLKAVCLKNGVYASRTLARIDLNEALSQPIYDQIDPVFGGIFRDGKPTAPALMQHIDAFKHSLEERM
  AEVGVRSGWKQDGYKRSFLIQEISAILGGLESHILRRKRKIYKSVTSSIQNDLKPCYEEAAQITGKKACE
  RMKDVIRRGVERQVAEGLFERAQERMWHQFRQLKHGITEKVKGSITTMLTLAAPQGVGLCKELADVRNEQ
  KEMEKLYRSLREVAENAQLRRSMQDFLLRMSPSKAGPHGTKL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001195674
• ORF Size: 2436 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001195674.2, NP_001182603.2
• RefSeq Size: 2567 bp
• RefSeq ORF: 2439 bp
• Locus ID: 100503545
• Cytogenetics: 14 D1
• Gene Summary: Nuclear GTPase found in germinal center B-cells, where it may inhibit function of the activation-induced cytidine deaminase AICDA (By similarity). Reduces somatic hypermutation in B-cells which may enhance genome stability (PubMed:22833677).[UniProtKB/Swiss-Prot Function]