Nuggc (NM_001195674) Mouse Untagged Clone

CAT#: MC221828

Nuggc (untagged) - Mouse predicted gene 600 (Gm600), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nuggc
• Synonyms: Gm600; SLIP-GC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221828 representing NM_001195674
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTTGGACTCTATGAAATCCAAGGAGGAGCTCGGAGTAGCACCTCTTGGGACCGGCTATTCCAGTT
  CATTCCAAGGCGTTTCCCCTGCTGACTGCGATCTGTTTAAAGAACCTGTTAGGAAAAGACGCAGGTTGCA
  TCGAGACCGGCAATTCCAGGCCTTTCCTTCTGCAGAGCAAAGTGCTCTTAAGGAATATGAAAAACTGGAG
  TGTCGGACCAGGAGGGTCTTGAGCAACACTTATCAGAAATTGATTCAGTCTGTCTTCCTGGATGACAGCA
  TTCCCAGCGGACTCAAGTATCTCATAAACCGGCTTCTTGCCTTGATTGAAAAATCCCCACTGGAGCCAGT
  CTATGTGGGGTTCTTGGGAATCACTGGAGCTGGCAAGAGCTCTCTGATCAATGCCCTCATCCGGCAAGCA
  ATGTTTCTGCCTGTGTCTGGAGAAAGTGTTTGCACATCATGCATCGTACAAGTGAGCTCTGGCTGCTGTG
  AGCAGTATGAGGCCAAAATTCACCTTCTGTCCGATCAGGAGTGGAAGGCAGAACTGAAGGACTTGACTAA
  GCTCCTGCACAGAGCAGAGCAGTCTGGGGAAGAGGAGGCAGACTTGTGGGACAGGGATGATGCAACGGAA
  GAAGCTGCCCAGAAGCTACGGATGCTCTATGGCCATGGGGCAGAAAGGAGGCACTACGAGGAGTTGCTAA
  GGCTGAAGCCCAGAGGGAGGATTCCCAACTCGAGAACCATCACTCTAAAGGCAGAGGAGGCAGGAGAGCT
  ATCTGTCAAACTGGACCCCTACATCAGGACCAGGAGGAGAGACTGGGATGGAGAATCAGCAGAGACACAA
  ATCTGGCCCTTGATCAAATATGTGGAAGTGATTCTTCCCAAATCTGCACTGATCCCAGAAGGGGTTGTCC
  TGGTAGACATTCCAGGCACAGGAGACTTCAACAGCAAGAGGGATAAAATGTGGAAAAAGACCATTGACAA
  GTGTTCCGTGATCTGGGTGATCAGCGACATCGAGCGAGTTTCTGGAGGAAAGACCCATGAAGACCTTCTG
  AGCGAGAGCATCAAAGCCTGCCAGCGTGGCTTCTGCAGGGACATCGCCCTGGTGGTCACCAAAACAGACA
  AACTCCACTTGCAGGAATATCTCAGGGAAAGAAAGATGGGAAATCAAGCTATTCAGAGTCAGAGAGAAGC
  TGTTCTGCAAAGAAATGAAATAATCAAACTACAAAGGAAGAGAATGCTCAAGGAGAAATTGAAGAGAAAA
  CTGCCAGCTGACTCCAAGGTTCTGGAAGCCTCAGATCTGGTGTACACGGTCAGTGCCCATGAGTACTGGC
  AGCGGACCATCCTGACTGAGGAAGAAAGTGAGATCCCCAAGTTAAGAGAATATATTAGGAAAAGGATCTT
  GGACAAGAAGAGGAGGCTGGTGACCAAGTACGTCACAGAAGCCTTTGGCCTATTGCTTCTCACAGACACG
  TTAAACACGGAGGAAAGCCTGCTGACTGAAGAACTGAACACGGGTGGCCTGCGACAATTTGTAGAGGAGA
  AGATGGAGCTGTTGGAGAAGGCCATCGAGCAGTGCTTTGCTCGAATGGAACAGCCCCTGCAGACAGGGGT
  CCAGGTGGCCATGACTTCCTACCGCCGCATCCTCGGGTCATGCCTGGTGAGGAGCAGAGGAAACCAAGGT
  TTTCACCAGACCCTGAAAGCAGTTTGCCTGAAAAATGGGGTCTATGCCTCCAGAACTCTGGCCAGAATTG
  ACCTGAATGAAGCCCTGTCTCAGCCTATCTATGACCAGATTGACCCTGTATTTGGAGGCATTTTTAGGGA
  TGGGAAGCCCACTGCCCCTGCTCTGATGCAGCACATAGATGCTTTCAAGCATTCCCTGGAGGAGAGGATG
  GCAGAAGTTGGGGTTAGAAGTGGCTGGAAACAAGATGGATACAAGAGAAGCTTCCTGATCCAGGAGATAA
  GCGCCATCCTGGGAGGCCTGGAGAGCCACATCCTCCGGAGGAAAAGGAAGATCTATAAGTCTGTCACCAG
  CTCCATCCAAAACGACCTGAAGCCCTGCTATGAAGAGGCAGCTCAGATCACTGGCAAGAAAGCATGCGAG
  AGAATGAAGGATGTCATCAGGAGAGGGGTGGAGCGGCAGGTGGCTGAGGGCTTGTTTGAGAGGGCTCAAG
  AGAGGATGTGGCACCAGTTTCGACAGCTGAAGCATGGAATCACTGAGAAGGTCAAGGGCAGCATCACTAC
  CATGTTAACCCTTGCTGCACCCCAGGGAGTTGGACTCTGCAAGGAGCTTGCAGATGTCCGGAATGAACAG
  AAGGAAATGGAAAAACTCTACAGAAGCCTGAGGGAGGTTGCTGAGAACGCACAGCTGCGGAGGAGCATGC
  AAGATTTCCTCCTGCGAATGTCCCCCAGCAAAGCCGGCCCCCATGGAACAAAACTTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001195674
• Insert Size: 2439 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001195674.2, NP_001182603.2
• RefSeq Size: 2567 bp
• RefSeq ORF: 2439 bp
• Locus ID: 100503545
• Cytogenetics: 14 D1
• Gene Summary: Nuclear GTPase found in germinal center B-cells, where it may inhibit function of the activation-induced cytidine deaminase AICDA (By similarity). Reduces somatic hypermutation in B-cells which may enhance genome stability (PubMed:22833677).[UniProtKB/Swiss-Prot Function]