Nfe2l2 (NM_010902) Mouse Tagged ORF Clone

CAT#: MG226717

Nfe2l2 (tGFP-tagged) - Mouse nuclear factor erythroid derived 2 like 2 (Nfe2l2), (10ug)

Product Images

Circular map for MG226717

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nfe2l2
• Synonyms: Nr; Nrf2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226717 representing NM_010902
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGACTTGGAGTTGCCACCGCCAGGACTACAGTCCCAGCAGGACATGGATTTGATTGACATCCTTT
  GGAGGCAAGACATAGATCTTGGAGTAAGTCGAGAAGTGTTTGACTTTAGTCAGCGACAGAAGGACTATGA
  GCTGGAAAAACAGAAAAAACTCGAAAAGGAAAGACAAGAGCAACTCCAGAAGGAACAGGAGAAGGCCTTT
  TTTGCTCAGTTTCAACTGGATGAAGAAACAGGAGAATTCCTCCCAATTCAGCCGGCCCAGCACATCCAGA
  CAGACACCAGTGGATCCGCCAGCTACTCCCAGGTTGCCCACATTCCCAAACAAGATGCCTTGTACTTTGA
  AGACTGTATGCAGCTTTTGGCAGAGACATTCCCATTTGTAGATGACCATGAGTCGCTTGCCCTGGATATC
  CCCAGCCACGCTGAAAGTTCAGTCTTCACTGCCCCTCATCAGGCCCAGTCCCTCAATAGCTCTCTGGAGG
  CAGCCATGACTGATTTAAGCAGCATAGAGCAGGACATGGAGCAAGTTTGGCAGGAGCTATTTTCCATTCC
  CGAATTACAGTGTCTTAATACCGAAAACAAGCAGCTGGCTGATACTACCGCTGTTCCCAGCCCAGAAGCC
  ACACTGACAGAAATGGACAGCAATTACCATTTTTACTCATCGATCTCCTCGCTGGAAAAAGAAGTGGGCA
  ACTGTGGTCCACATTTCCTTCATGGTTTTGAGGATTCTTTCAGCAGCATCCTCTCCACTGATGATGCCAG
  CCAGCTGACCTCCTTAGACTCAAATCCCACCTTAAACACAGATTTTGGCGATGAATTTTATTCTGCTTTC
  ATAGCAGAGCCCAGTGACGGTGGCAGCATGCCTTCCTCCGCTGCCATCAGTCAGTCACTCTCTGAACTCC
  TGGACGGGACTATTGAAGGCTGTGACCTGTCACTGTGTAAAGCTTTCAACCCGAAGCACGCTGAAGGCAC
  AATGGAATTCAATGACTCTGACTCTGGCATTTCACTGAACACGAGTCCCAGCCGAGCGTCCCCAGAGCAC
  TCCGTGGAGTCTTCCATTTACGGAGACCCACCGCCTGGGTTCAGTGACTCGGAAATGGAGGAGCTAGATA
  GTGCCCCTGGAAGTGTCAAACAGAACGGCCCTAAAGCACAGCCAGCACATTCTCCTGGAGACACAGTACA
  GCCTCTGTCACCAGCTCAAGGGCACAGTGCTCCTATGCGTGAATCCCAATGTGAAAATACAACAAAAAAA
  GAAGTTCCCGTGAGTCCTGGTCATCAAAAAGCCCCATTCACAAAAGACAAACATTCAAGCCGCTTAGAGG
  CTCATCTCACACGAGATGAGCTTAGGGCAAAAGCTCTCCATATTCCATTCCCTGTCGAAAAAATCATTAA
  CCTCCCTGTTGATGACTTCAATGAAATGATGTCCAAGGAGCAATTCAATGAAGCTCAGCTCGCATTGATC
  CGAGATATACGCAGGAGAGGTAAGAATAAAGTCGCCGCCCAGAACTGTAGGAAAAGGAAGCTGGAGAACA
  TTGTCGAGCTGGAGCAAGACTTGGGCCACTTAAAAGACGAGAGAGAAAAACTACTCAGAGAAAAGGGAGA
  AAACGACAGAAACCTCCATCTACTGAAAAGGCGGCTCAGCACCTTGTATCTTGAAGTCTTCAGCATGTTA
  CGTGATGAGGATGGAAAGCCTTACTCTCCCAGTGAATACTCTCTGCAGCAAACCAGAGATGGCAATGTGT
  TCCTTGTTCCCAAAAGCAAGAAGCCAGATACAAAGAAAAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226717 representing NM_010902
  Red=Cloning site Green=Tags(s)
  
  MMDLELPPPGLQSQQDMDLIDILWRQDIDLGVSREVFDFSQRQKDYELEKQKKLEKERQEQLQKEQEKAF
  FAQFQLDEETGEFLPIQPAQHIQTDTSGSASYSQVAHIPKQDALYFEDCMQLLAETFPFVDDHESLALDI
  PSHAESSVFTAPHQAQSLNSSLEAAMTDLSSIEQDMEQVWQELFSIPELQCLNTENKQLADTTAVPSPEA
  TLTEMDSNYHFYSSISSLEKEVGNCGPHFLHGFEDSFSSILSTDDASQLTSLDSNPTLNTDFGDEFYSAF
  IAEPSDGGSMPSSAAISQSLSELLDGTIEGCDLSLCKAFNPKHAEGTMEFNDSDSGISLNTSPSRASPEH
  SVESSIYGDPPPGFSDSEMEELDSAPGSVKQNGPKAQPAHSPGDTVQPLSPAQGHSAPMRESQCENTTKK
  EVPVSPGHQKAPFTKDKHSSRLEAHLTRDELRAKALHIPFPVEKIINLPVDDFNEMMSKEQFNEAQLALI
  RDIRRRGKNKVAAQNCRKRKLENIVELEQDLGHLKDEREKLLREKGENDRNLHLLKRRLSTLYLEVFSML
  RDEDGKPYSPSEYSLQQTRDGNVFLVPKSKKPDTKKN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010902
• ORF Size: 1791 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010902.4
• RefSeq Size: 2469 bp
• RefSeq ORF: 1794 bp
• Locus ID: 18024
• UniProt ID: Q60795
• Cytogenetics: 2 44.75 cM
• Gene Summary: This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. [provided by RefSeq, Sep 2015]