Nfe2l2 (NM_010902) Mouse Untagged Clone

CAT#: MC219509

Nfe2l2 (untagged) - Mouse nuclear factor, erythroid derived 2, like 2 (Nfe2l2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nfe2l2
• Synonyms: Nr; Nrf2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219509 representing NM_010902
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGACTTGGAGTTGCCACCGCCAGGACTACAGTCCCAGCAGGACATGGATTTGATTGACATCCTTT
  GGAGGCAAGACATAGATCTTGGAGTAAGTCGAGAAGTGTTTGACTTTAGTCAGCGACAGAAGGACTATGA
  GCTGGAAAAACAGAAAAAACTCGAAAAGGAAAGACAAGAGCAACTCCAGAAGGAACAGGAGAAGGCCTTT
  TTTGCTCAGTTTCAACTGGATGAAGAAACAGGAGAATTCCTCCCAATTCAGCCGGCCCAGCACATCCAGA
  CAGACACCAGTGGATCCGCCAGCTACTCCCAGGTTGCCCACATTCCCAAACAAGATGCCTTGTACTTTGA
  AGACTGTATGCAGCTTTTGGCAGAGACATTCCCATTTGTAGATGACCATGAGTCGCTTGCCCTGGATATC
  CCCAGCCACGCTGAAAGTTCAGTCTTCACTGCCCCTCATCAGGCCCAGTCCCTCAATAGCTCTCTGGAGG
  CAGCCATGACTGATTTAAGCAGCATAGAGCAGGACATGGAGCAAGTTTGGCAGGAGCTATTTTCCATTCC
  CGAATTACAGTGTCTTAATACCGAAAACAAGCAGCTGGCTGATACTACCGCTGTTCCCAGCCCAGAAGCC
  ACACTGACAGAAATGGACAGCAATTACCATTTTTACTCATCGATCTCCTCGCTGGAAAAAGAAGTGGGCA
  ACTGTGGTCCACATTTCCTTCATGGTTTTGAGGATTCTTTCAGCAGCATCCTCTCCACTGATGATGCCAG
  CCAGCTGACCTCCTTAGACTCAAATCCCACCTTAAACACAGATTTTGGCGATGAATTTTATTCTGCTTTC
  ATAGCAGAGCCCAGTGACGGTGGCAGCATGCCTTCCTCCGCTGCCATCAGTCAGTCACTCTCTGAACTCC
  TGGACGGGACTATTGAAGGCTGTGACCTGTCACTGTGTAAAGCTTTCAACCCGAAGCACGCTGAAGGCAC
  AATGGAATTCAATGACTCTGACTCTGGCATTTCACTGAACACGAGTCCCAGCCGAGCGTCCCCAGAGCAC
  TCCGTGGAGTCTTCCATTTACGGAGACCCACCGCCTGGGTTCAGTGACTCGGAAATGGAGGAGCTAGATA
  GTGCCCCTGGAAGTGTCAAACAGAACGGCCCTAAAGCACAGCCAGCACATTCTCCTGGAGACACAGTACA
  GCCTCTGTCACCAGCTCAAGGGCACAGTGCTCCTATGCGTGAATCCCAATGTGAAAATACAACAAAAAAA
  GAAGTTCCCGTGAGTCCTGGTCATCAAAAAGCCCCATTCACAAAAGACAAACATTCAAGCCGCTTAGAGG
  CTCATCTCACACGAGATGAGCTTAGGGCAAAAGCTCTCCATATTCCATTCCCTGTCGAAAAAATCATTAA
  CCTCCCTGTTGATGACTTCAATGAAATGATGTCCAAGGAGCAATTCAATGAAGCTCAGCTCGCATTGATC
  CGAGATATACGCAGGAGAGGTAAGAATAAAGTCGCCGCCCAGAACTGTAGGAAAAGGAAGCTGGAGAACA
  TTGTCGAGCTGGAGCAAGACTTGGGCCACTTAAAAGACGAGAGAGAAAAACTACTCAGAGAAAAGGGAGA
  AAACGACAGAAACCTCCATCTACTGAAAAGGCGGCTCAGCACCTTGTATCTTGAAGTCTTCAGCATGTTA
  CGTGATGAGGATGGAAAGCCTTACTCTCCCAGTGAATACTCTCTGCAGCAAACCAGAGATGGCAATGTGT
  TCCTTGTTCCCAAAAGCAAGAAGCCAGATACAAAGAAAAACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010902
• Insert Size: 1794 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010902.3, NP_035032.1
• RefSeq Size: 2469 bp
• RefSeq ORF: 1794 bp
• Locus ID: 18024
• UniProt ID: Q60795
• Cytogenetics: 2 44.75 cM
• Gene Summary: This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. [provided by RefSeq, Sep 2015]
  Transcript Variant: This variant (1) encodes the protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.