Cd44 (NM_001177787) Mouse Tagged ORF Clone

CAT#: MG226618

Cd44 (tGFP-tagged) - Mouse CD44 antigen (Cd44) transcript variant 6, (10ug)

Product Images

Circular map for MG226618

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cd44
• Synonyms: AU023126; AW121933; AW146109; HERMES; Ly-24; Pgp-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226618 representing NM_001177787
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAAGTTTTGGTGGCACACAGCTTGGGGACTTTGCCTCTTGCAGTTGAGCCTGGCACATCCACATC
  AGCAGATCGATTTGAATGTAACCTGCCGCTACGCAGGTGTATTCCATGTGGAGAAAAATGGCCGCTACAG
  TATCTCCCGGACTGAGGCAGCTGACCTCTGCCAGGCTTTCAACAGTACCTTACCCACCATGGACCAAATG
  AAGTTGGCCCTGAGCAAGGGTTTTGAAACATGCAGGTATGGGTTCATAGAAGGAAATGTGGTAATTCCGA
  GGATTCATCCCAACGCTATCTGTGCAGCCAACCACACAGGAGTATATATCCTCGTCACGTCCAACACCTC
  CCACTATGACACATATTGCTTCAATGCCTCAGCCCCTCCTGAAGAAGACTGTACATCAGTCACAGACCTA
  CCCAATTCCTTCGATGGACCGGTTACCATAACTATTGTCAACCGTGATGGTACTCGCTACAGCAAGAAGG
  GCGAGTATAGAACACACCAAGAAGACATCGATGCTTCAAACATTATAGATGACGATGTCAGCAGCGGCTC
  CACCATCGAGAAGAGCACCCCAGAAAGCTACATTTTGCACACCTACCTTCCTACTGAACAGCCTACTGGA
  GATCAGGATGACTCCTTCTTTATCCGGAGCACCTTGGCCACCACACAGAGTCATTCTCAGAACTTCTCTA
  CATTACATGGAGAGCCGGAAGAAGACGAAAACCATCCAACAACTTCTATTCTGCCATCTAGCACTAAGAG
  CGGCGCTAAAGATGCAAGAAGAGGTGGAAGTCTTCCCACAGATACAACTACTTCTGTAGAAGGCTACACC
  TTTCAATACCCAGACACAATGGAAAATGGGACTCTCTTCCCAGTGACCCCTGCTAAAACTGAGGTCTTTG
  GAGAAACTGAAGTGACTCTTGCTACTGACTCCAACGTTAATGTTGATGGCTCCTTACCAGGAGATCGAGA
  CTCATCCAAGGACTCCAGGGGGAGTTCCCGCACTGTGACTCATGGATCCGAATTAGCTGGACACTCAAGT
  GCGAACCAGGACAGTGGAGTGACCACAACTTCTGGTCCTATGAGGAGACCTCAGATTCCAGAATGGCTCA
  TCATCTTGGCATCTCTCCTGGCACTGGCTCTGATTCTTGCCGTCTGCATCGCGGTCAATAGTAGGAGAAG
  GTGTGGGCAGAAGAAAAAGCTGGTGATCAACGGTGGCAATGGGACGGTGGAAGACAGGAAACCCAGTGAG
  CTCAACGGGGAGGCCAGCAAGTCTCAGGAAATGGTGCATTTGGTGAACAAGGAACCATCAGAGACCCCGG
  ACCAGTGTATGACAGCTGACGAGACCCGGAATCTGCAGAGTGTGGACATGAAGATTGGGGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226618 representing NM_001177787
  Red=Cloning site Green=Tags(s)
  
  MDKFWWHTAWGLCLLQLSLAHPHQQIDLNVTCRYAGVFHVEKNGRYSISRTEAADLCQAFNSTLPTMDQM
  KLALSKGFETCRYGFIEGNVVIPRIHPNAICAANHTGVYILVTSNTSHYDTYCFNASAPPEEDCTSVTDL
  PNSFDGPVTITIVNRDGTRYSKKGEYRTHQEDIDASNIIDDDVSSGSTIEKSTPESYILHTYLPTEQPTG
  DQDDSFFIRSTLATTQSHSQNFSTLHGEPEEDENHPTTSILPSSTKSGAKDARRGGSLPTDTTTSVEGYT
  FQYPDTMENGTLFPVTPAKTEVFGETEVTLATDSNVNVDGSLPGDRDSSKDSRGSSRTVTHGSELAGHSS
  ANQDSGVTTTSGPMRRPQIPEWLIILASLLALALILAVCIAVNSRRRCGQKKKLVINGGNGTVEDRKPSE
  LNGEASKSQEMVHLVNKEPSETPDQCMTADETRNLQSVDMKIGV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001177787
• ORF Size: 1392 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177787.1, NP_001171258.1
• RefSeq Size: 4706 bp
• RefSeq ORF: 1395 bp
• Locus ID: 12505
• UniProt ID: P15379
• Cytogenetics: 2 54.13 cM
• Gene Summary: Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment. Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection. Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases (PubMed:8343954, PubMed:25065622). Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion (By similarity).[UniProtKB/Swiss-Prot Function]