Dvl1 (NM_010091) Mouse Tagged ORF Clone

CAT#: MG225845

Dvl1 (tGFP-tagged) - Mouse dishevelled dsh homolog 1 (Drosophila) (Dvl1), (10ug)

Product Images

Circular map for MG225845

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dvl1
• Synonyms: Dvl; mKIAA4029
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225845 representing NM_010091
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGACCAAAATCATCTACCACATGGACGAGGAGGAGACGCCGTACCTGGTCAAGCTGCCCGTAG
  CTCCCGAGCGCGTCACGCTGGCCGACTTCAAGAACGTGCTCAGCAACCGGCCGGTGCACGCCTACAAATT
  CTTCTTCAAGTCCATGGACCAGGACTTCGGGGTGGTGAAGGAGGAGATCTTCGATGACAATGCCAAGTTG
  CCCTGCTTCAATGGCCGGGTGGTTTCCTGGCTGGTCCTGGCTGAGGGCGCTCACTCGGATGCAGGGTCCC
  AGGGCACTGACAGCCACACGGACCTGCCCCCACCCCTTGAGAGGACAGGCGGCATTGGGGACTCCAGGCC
  CCCCTCCTTCCATCCAAATGTTGCCAGTAGCCGGGACGGAATGGACAATGAGACAGGCACAGAGTCTATG
  GTCAGTCACCGGCGGGAGCGAGCCCGACGTCGAAACCGCGATGAGGCTGCCCGGACCAATGGGCACCCGA
  GAGGGGATCGGCGGCGGGACCTGGGACTACCTCCAGACAGTGCATCTACTGTACTGAGCAGTGAGCTTGA
  ATCTAGCAGCTTTATTGACTCAGATGAGGAGGACAATACGAGCCGGCTGAGCAGCTCCACAGAGCAGAGC
  ACCTCCTCTCGGCTAGTTCGGAAGCACAAATGCCGTCGTCGGAAGCAGCGCTTGAGGCAGACAGACCGGG
  CATCCTCCTTCAGCAGCATCACAGACTCCACCATGTCCCTGAACATCATCACCGTCACTCTCAACATGGA
  GAGGCACCACTTCCTGGGCATCAGCATCGTGGGCCAGAGCAACGACCGGGGTGATGGCGGCATCTACATT
  GGATCCATCATGAAGGGCGGGGCCGTGGCTGCTGATGGCCGCATTGAGCCGGGCGACATGTTGCTGCAGG
  TGAACGATGTCAACTTTGAGAACATGAGCAATGACGACGCTGTACGGGTGCTTCGGGAGATCGTGTCCCA
  GACAGGGCCCATCAGTCTCACAGTGGCCAAGTGCTGGGACCCAACCCCTCGGAGCTACTTCACCATCCCA
  AGGGCTGACCCAGTGCGACCCATCGACCCGGCTGCCTGGCTGTCCCACACAGCAGCACTGACGGGTGCCC
  TGCCCCGCTATGGTACGAGTCCCTGCTCCAGCGCCATCACACGCACCAGCTCTTCCTCACTAACCAGCTC
  AGTGCCTGGCGCCCCACAGCTTGAGGAGGCGCCGCTGACTGTGAAGAGTGACATGAGTGCCATTGTCCGC
  GTCATGCAGTTGCCAGACTCAGGACTGGAGATCCGGGACCGCATGTGGCTTAAGATCACCATTGCCAATG
  CTGTCATTGGGGCGGATGTGGTGGACTGGCTGTACACACACGTGGAGGGCTTCAAGGAGCGAAGGGAGGC
  AAGAAAGTATGCCAGCAGTATGCTGAAGCACGGTTTCCTGAGGCACACCGTGAACAAGATCACCTTTTCT
  GAGCAGTGCTACTATGTCTTTGGCGACCTGTGCAGTAACCTCGCATCCCTGAACCTCAACAGTGGCTCCA
  GTGGAGCCTCAGATCAGGACACACTGGCCCCACTGCCCCACCCATCAGTACCCTGGCCCTTGGGTCAAGG
  CTACCCCTACCAGTACCCAGGACCCCCGCCCTGCTTCCCACCTGCTTACCAGGACCCTGGCTTCAGCTGC
  GGCAGCGGCAGTGCTGGGAGCCAGCAGAGTGAAGGGAGCAAGAGCAGTGGGTCCACACGGAGCAGCCATC
  GGACCCCAGGCCGAGAGGAGCGCCGGGCAACTGGAGCTGGGGGTAGTGGCAGTGAATCAGACCACACAGT
  ACCAAGTGGGTCTGGTAGCACCGGCTGGTGGGAGCGGCCTGTCAGCCAGCTTAGCCGTGGCAGTAGCCCT
  CGAAGTCAGGCTTCAGCTGTTGCCCCAGGGCTCCCCCCACTGCACCCCCTTACAAAGGCCTATGCAGTAG
  TGGGTGGGCCACCTGGAGGGCCACCTGTCCGGGAGCTGGCTGCTGTCCCTCCAGAACTTACAGGTAGCCG
  CCAGTCCTTCCAAAAGGCCATGGGAAACCCCTGTGAGTTCTTTGTGGACATCATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225845 representing NM_010091
  Red=Cloning site Green=Tags(s)
  
  MAETKIIYHMDEEETPYLVKLPVAPERVTLADFKNVLSNRPVHAYKFFFKSMDQDFGVVKEEIFDDNAKL
  PCFNGRVVSWLVLAEGAHSDAGSQGTDSHTDLPPPLERTGGIGDSRPPSFHPNVASSRDGMDNETGTESM
  VSHRRERARRRNRDEAARTNGHPRGDRRRDLGLPPDSASTVLSSELESSSFIDSDEEDNTSRLSSSTEQS
  TSSRLVRKHKCRRRKQRLRQTDRASSFSSITDSTMSLNIITVTLNMERHHFLGISIVGQSNDRGDGGIYI
  GSIMKGGAVAADGRIEPGDMLLQVNDVNFENMSNDDAVRVLREIVSQTGPISLTVAKCWDPTPRSYFTIP
  RADPVRPIDPAAWLSHTAALTGALPRYGTSPCSSAITRTSSSSLTSSVPGAPQLEEAPLTVKSDMSAIVR
  VMQLPDSGLEIRDRMWLKITIANAVIGADVVDWLYTHVEGFKERREARKYASSMLKHGFLRHTVNKITFS
  EQCYYVFGDLCSNLASLNLNSGSSGASDQDTLAPLPHPSVPWPLGQGYPYQYPGPPPCFPPAYQDPGFSC
  GSGSAGSQQSEGSKSSGSTRSSHRTPGREERRATGAGGSGSESDHTVPSGSGSTGWWERPVSQLSRGSSP
  RSQASAVAPGLPPLHPLTKAYAVVGGPPGGPPVRELAAVPPELTGSRQSFQKAMGNPCEFFVDIM
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010091
• ORF Size: 2085 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010091.3, NP_034221.3
• RefSeq Size: 3365 bp
• RefSeq ORF: 2088 bp
• Locus ID: 13542
• UniProt ID: P51141
• Cytogenetics: 4 87.61 cM
• Gene Summary: The protein encoded by this gene is a member of the Dishevelled family of proteins. These proteins have an N-terminal Dishevelled/Axin domain, a Dishevelled, EGL-10, Plextrin domain, a central PDZ domain, and a C-terminal domain of approximately 200 amino acids. They regulate both canonical and non-canonical Wnt signaling as well as planar cell polarity pathways. Mice deficient for this gene are viable and fertile but display reduced social interaction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]