Dvl1 (NM_010091) Mouse Untagged Clone

CAT#: MC220568

Dvl1 (untagged) - Mouse dishevelled, dsh homolog 1 (Drosophila) (Dvl1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dvl1
• Synonyms: Dvl; mKIAA4029
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220568 representing NM_010091
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGACCAAAATCATCTACCACATGGACGAGGAGGAGACGCCGTACCTGGTCAAGCTGCCCGTAG
  CTCCCGAGCGCGTCACGCTGGCCGACTTCAAGAACGTGCTCAGCAACCGGCCGGTGCACGCCTACAAATT
  CTTCTTCAAGTCCATGGACCAGGACTTCGGGGTGGTGAAGGAGGAGATCTTCGATGACAATGCCAAGTTG
  CCCTGCTTCAATGGCCGGGTGGTTTCCTGGCTGGTCCTGGCTGAGGGCGCTCACTCGGATGCAGGGTCCC
  AGGGCACTGACAGCCACACGGACCTGCCCCCACCCCTTGAGAGGACAGGCGGCATTGGGGACTCCAGGCC
  CCCCTCCTTCCATCCAAATGTTGCCAGTAGCCGGGACGGAATGGACAATGAGACAGGCACAGAGTCTATG
  GTCAGTCACCGGCGGGAGCGAGCCCGACGTCGAAACCGCGATGAGGCTGCCCGGACCAATGGGCACCCGA
  GAGGGGATCGGCGGCGGGACCTGGGACTACCTCCAGACAGTGCATCCACTGTACTGAGCAGTGAGCTTGA
  ATCTAGCAGCTTTATTGACTCAGATGAGGAGGACAATACGAGCCGGCTGAGCAGCTCCACAGAGCAGAGC
  ACCTCCTCTCGGCTAGTTCGGAAGCACAAATGCCGTCGTCGGAAGCAGCGCTTGAGGCAGACAGACCGGG
  CATCCTCCTTCAGCAGCATCACAGACTCCACCATGTCCCTGAACATCATCACCGTCACTCTCAACATGGA
  GAGGCACCACTTCCTGGGCATCAGCATCGTGGGCCAGAGCAACGACCGGGGTGATGGCGGCATCTACATT
  GGATCCATCATGAAGGGCGGGGCCGTGGCTGCTGATGGCCGCATTGAGCCGGGCGACATGTTGCTGCAGG
  TGAACGATGTCAACTTTGAGAACATGAGCAATGACGACGCTGTACGGGTGCTTCGGGAGATCGTGTCCCA
  GACAGGGCCCATCAGTCTCACAGTGGCCAAGTGCTGGGACCCAACCCCTCGGAGCTACTTCACCATCCCA
  AGGGCTGACCCAGTGCGACCCATCGACCCGGCTGCCTGGCTGTCCCACACAGCAGCACTGACGGGTGCCC
  TGCCCCGCTATGGTACGAGTCCCTGCTCCAGCGCCATCACACGCACCAGCTCTTCCTCACTAACCAGCTC
  AGTGCCTGGCGCCCCACAGCTTGAGGAGGCACCGCTGACTGTGAAGAGTGACATGAGTGCCATTGTCCGC
  GTCATGCAGTTGCCAGACTCAGGACTGGAGATCCGGGACCGCATGTGGCTTAAGATCACCATTGCCAATG
  CTGTCATTGGGGCGGATGTGGTGGACTGGCTGTACACACACGTGGAGGGCTTCAAGGAGCGAAGGGAGGC
  AAGAAAGTATGCCAGCAGTATGCTGAAGCACGGTTTCCTGAGGCACACCGTGAACAAGATCACCTTTTCT
  GAGCAGTGCTACTATGTCTTTGGCGACCTGTGCAGTAACCTCGCATCCCTGAACCTCAACAGTGGCTCCA
  GTGGAGCCTCAGATCAGGACACACTGGCCCCACTGCCCCACCCATCAGTACCCTGGCCCTTGGGTCAAGG
  CTACCCCTACCAGTACCCAGGACCCCCGCCCTGCTTCCCGCCTGCTTACCAGGACCCTGGCTTCAGCTGC
  GGCAGCGGCAGTGCTGGGAGCCAGCAGAGTGAAGGGAGCAAGAGCAGTGGGTCCACACGGAGCAGCCATC
  GGACCCCAGGCCGAGAGGAGCGCCGGGCAACTGGAGCTGGGGGTAGTGGCAGTGAATCAGACCACACAGT
  ACCAAGTGGGTCTGGTAGCACCGGCTGGTGGGAGCGGCCTGTCAGCCAGCTTAGCCGTGGCAGTAGCCCT
  CGAAGTCAGGCTTCAGCTGTTGCCCCAGGGCTCCCCCCACTGCACCCCCTTACAAAGGCCTATGCAGTAG
  TGGGTGGGCCACCTGGAGGGCCACCCGTCCGGGAGCTGGCTGCTGTCCCTCCAGAACTTACAGGTAGCCG
  CCAGTCCTTCCAAAAGGCCATGGGAAACCCCTGTGAGTTCTTTGTGGACATCATGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010091
• Insert Size: 2088 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010091.4, NP_034221.3
• RefSeq Size: 3459 bp
• RefSeq ORF: 2088 bp
• Locus ID: 13542
• UniProt ID: P51141
• Cytogenetics: 4 87.61 cM
• Gene Summary: The protein encoded by this gene is a member of the Dishevelled family of proteins. These proteins have an N-terminal Dishevelled/Axin domain, a Dishevelled, EGL-10, Plextrin domain, a central PDZ domain, and a C-terminal domain of approximately 200 amino acids. They regulate both canonical and non-canonical Wnt signaling as well as planar cell polarity pathways. Mice deficient for this gene are viable and fertile but display reduced social interaction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]