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Dysf (NM_001077694) Mouse Tagged ORF Clone
CAT#: MG225180
- TrueORF®
Dysf (tGFP-tagged) - Mouse dysferlin (Dysf) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_001077694" in other vectors (2)
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Symbol | Dysf |
| Synonyms | 2310004N10Rik; AI604795; D6Pas3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG225180 representing NM_001077694
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCGAGTCTTCATCCTTTTTGCGGAGAATGTCCACACCCCGGACTCCGACATCAGCGATGCCTACT GCTCCGCGGTGTTTGCAGGGGTAAAGAAGAGAACCAAAGTCATCAAGAACAGTGTGAACCCCGTGTGGAA TGAGGGCTTTGAGTGGGACCTCAAAGGTATTCCTCTAGATCAGAGCTCAGAACTTCTCGTGGTGGTCAAG GACCATGAGACGATGGGAAGAAACAGGTTCCTGGGGGAAGCCAAGATCCCACTCCAGGAGGTCCTTGCCA CCCCCAGCCTCTCTGCCAGCTTCAACGCACCTCTGCTAGACGCCAAGCAGCAACCCACGGGGGCCTCTCT GGTCCTGCAGGTGTCCTACACGCCACCCCCAGGAGCTGTGCCCCTGTTCCCACCACCTGCTTCTCTAGCA CCCTCCCCGACACTGCCTGACATGGATCTGGTGCCTGGTGGGGGACAGAGCCGGGCTGAGACTTGGTCCC TGCTCAGTGACAGCACCATGGACACGAGATACTCTGGGAAGAAGTGGCCGGTCCCCACGGACACAGGAGG GGAGGAAGACACCGAGGACCAGGGCCTGACTGGAGATGAAGCTGAGCCGTTCCTGGACCAGAGTGCTGCT GTGGGCCCAGGGGGACCGACCACACCCAGGAAGCCACCTTCCCACCCTCCTCCCCACTATCCAGGGGCAA AAAGAAAAAGAAGCTCTGCCCCACCTAGAAAGCTGCTGTCAGACAAGCCACAGGACTTCCAGATCAGAGT CCAGGTGATAGAGGGGCGTCAGCTGCCTGGGGTGAATATTAAGCCTGTGGTCAAGGTCACAGCTGCTGGG CAGACCAAGCGAACTCGGATCCAGAAGGGAAATAGCCCACTCTTCAATGAGACTCTTTTCTTCAACGTAT TTGACTCTCCCTTGGAGCTGTTTGATGAGCCCATCTTCATCACGGTGGTGGACTCACGCTCCCTTAGGAC AGATGCCCTCCTTGGCGAGTTCCGGATGGATGTGGGCACCGTGTACAGAGAGCCCCGACACGCTTATCTC CGGAAGTGGCTGCTGCTCTCCGACCCTGATGACTTCTCTGCGGGGGCCAGAGGCTACCTCAAAGCCAGCC TGTGTGTGCTAGGGCCTGGAGATGAAGCTCCCCTGGACAAGAAGGACCCTTCTGAAGACAAAGAGGACAT TGAAGGCAACCTGCTTAGGCCCACCGGTGTGGCCCTTCGAGGAGCCCACTTCTGCCTGAAGCTCTTCCGG GCTGAGGACTTACCACAGATGGACGATGCTGTGATGGACAACGTCAAGCAGATCTTTGGCTTTGACAGCA ACAAGAAGAACTTGGTGGATCCCTTTGTGGAAGTCAGCTTTGCTGGGAAAATGCTCTGCAGCAAGATCCT GGAGAAGACAGCTAACCCTCAGTGGAACCAGAACATCACCTTGCCTGCGATGTTTCCCTCTATGTGTGAA AAAATGAGGATTCGTGTCATGGACTGGGACCGCCTCACTCACAATGACACTGTGGCCACCACCTACTTGG GTATGTCGAAAATCTCTGCTACTGGAGGAGAAATAGAAGTGGACGACAACCTGGGCTTCCTCCCCACTTT TGGGCCCTGCTATGTGAACCTCTATGGCAGTCCCCGAGAGTTCACGGGCTTCCCAGATCCTTATGCAGAG CTCAACACAGGAAAGGGAGAAGGTGTGGCTTATCGAGGCAGGGTTCTGTTGTCCCTGGAGACCAAACTGG TGGAACACAGTGAGCAGAAGGTGGAAGACCTTCCTGCTGATGATATCCTGCGTGTGGAGAAGTACCTGCG GAGGCGCAAATATTCCCTCTTTGCTGCCTTCTACTCAGCCACAATGCTGCAGGATGTGGATGACGCCATC CAGTTCGAGGTCAGCATTGGGAACTACGGGAACAAGTTCGACACCACCTGCCTGCCCTTGGCCTCCACCA CCCAGTACAGCCGGGCGGTCTTTGATGGATGCCACTACTATTACTTGCCTTGGGGCAACGTGAAGCCCGT GGTGGTGCTGTCCTCATACTGGGAAGACATCAGCCATCGAATTGAGATCCAAAACCAGCTCCTCAGGGTC GCTGACCGCCTGGAAGCTAACCTGGAGCAGGTCCACTTGGCTCTGAAGGCACAGTGTTCCTCCGAGGATG TGGACGCCCTGGTGGCTCAGTTGACAGATGAGCTCCTTGCAGACTGCAGCCAGCCCCTGTGTGACATCCA CGAGATACCTTCTGCCACCCACCTTGATCAGTACCTGCTCCGGCTACGCACCCGTCACCTGAGCCAGATC AAAGAAGCTGCTCTGGCCTTGAAGCTTGGCCATAGTGAGCTCTCCACTGCCCTGGAGCAGGCCGAGGACT GGCTTCTGCATCTCCGTGCCCTGGCCGAGGAGCCCCAGAACAGCCTGCCGGACATCATCATCTGGATGCT GCAGGGTGACAAGCGTGTGGCCTACCAGCGGGTGCCTGCCCATGAAGTCCTCTTCTCCCGGCGGGGCCCC AGCTACTGCGGCAGGAACTGTGGGAAGCTGCAGACCATCTTTCTGAAATATCCTATGGAAGGTATGCCTG GGGCCCGAATGCCCGTGCAGATTCGGATCAAGCTCTGGTTCGGGCTCTCTGTGGATGAGAAGGAATTCAA TCAGTTTGCTGAGGGAAAGCTCTCTGTCTTTGCCGAAACGTATGAGAACCAGACAAAGCTGGCACTGGTC GGGAACTGGGGCACAACGGGCCTCACCTACCCCAAGTTTTCTGATGTCACAGGCAAGATCAAACTACCAA AGGATAGCTTCCGCCCCTCAGCTGGCTGGGCCTGGGCAGGAGATTGGTTCGTGTGTCCAGAGAAGACCCT ACTCCATGATGCTGACGCTGGTCATCTGAGCTTCGTGGAGGAGGTGTTCGAGAACCAGACCCGGCTCCCT GGGGGCCAGTGGATTTACATGAGTGATAACTACACAGATGTGAATGGGGAGAAGGTACTTCCGAAGGATG ATATTGAATGCCCGCTGGGCTGGAAGTGGGAAGATGAAGAATGGTCCACAGACCTCAATCGGGCTGTTGA TGAACAAGGCTGGGAGTACAGCATCACCATACCCCCAGACCGAAAGCCAAAACACTGGGTCCCTGTTGAG AAGATGTATTACACTCACAGGCGTCGACGCTGGGTCCGACTGCGCAGGAGGGACCTCAGCCAGATGGAAG CACTGAAGAGGCACAGGCAGGCAGAGGCCGAGGGCGAGGGCTGGGAGTATGCCTCTCTCTTCGGCTGGAA GTTCCACCTGGAGTACCGCAAGACAGATGCCTTCCGCCGCCGCCGCTGGCGCCGCCGCATGGAGCCGCTG GAGAAGACGGGACCTGCAGCTGTGTTTGCCCTCGAGGGGGCCCTGGGCGGCATGGTGGATGACAAGAGCG AAGACTCCATGTCTGTTTCTACCCTGAGCTTTGGCGTTAACAGACCCACAATCTCCTGCATCTTTGACTA TGGGAACCGCTACCATCTGCGCTGCTACCTGTACCAGGCCCGAGACCTGCCAGCCATGGACAAGGACTCC TTCTCTGATCCCTACGCCATCGTCTCCTTCCTGCATCAAAGCCAGAAGACAGTGGTGGAGAAGAACACCC TGAATCCCACCTGGGATCAAACCCTCATCTTCTATGAGATTGAGATCTTCGGCGAGCCAGCCAGTATCGC CGAGCATCCGCCCTGCATCGTGGTGGAGCTCTATGACCACGACACCTATGGTGCTGACGAGTTCATGGGT CGCTGTATTTGTCAGCCAAGCCTGGAACGGATGCCCCGCCTGGCCTGGTTCCCACTGACCCGGGGCAGCC AGCCTGCCGGCGAGCTGCTGGCAGCGTTCGAGCTTATCCAGAGAGAGAAGCCAGCCATCCATCACATCCC TGGCTTTGAGATGCATGAAACTTCAAGGATTTTGGATGAGACGGAGGACACAGATTTGCCTTACCCACCA CCTCAAAGGGAGGCCAACATCTACATGGTTCCCCAGAACATTAAGCCAGCTCTCCAACGTACTGCCATTG AGATCCTGGCCTGGGGCCTGCGGAACATGAAGAGCTATCAGATGGCCAGCATCTCTTCCCCCAGCCTCGT GGTGGAGTGTGGTGGGCAGACAGTGCAGTCCTGTGTCATCAGAAACCTTCGGAAGAACCCCAACTTTGAT GTCTGTACTCTCTTCATGGAAGTGATGTTACCCAGAGAGGACCTCTACTGTCCACCTATTGTGGTCAAGG TCATTGATAATCGCCAGTTTGGCCGCCGGCCAGTTGTGGGTCAGTGTACCATTCGCTCCCTGGAGAACTT CCTGTGCGACCCATACTCAGCAGAGAGTCCATCCCCACAGGGTGGCCCAGATGATGTGAGCTTACTCAGC CCCGGGGAAGATGTGCTGATTGACATCGATGACAAGGAGCCTCTCATTCCTGTCCAGGAGGAGGAGTTCA TTGACTGGTGGAGCAAATTCTTTGCTTCTGTCGGGGAGAGGGAAAAGTGTGGTTCCTACCTGGAGAAGGA TTTTGACACCTTGAAGGTCTATGACACACAGCTGGAGAATGTGGAGGCTTTTGGAGGCCTGTCCGACTTT TGTAACACCTTCAAGCTCTACCGGGGCAGGACTCAGGAGGAGACAGATGATCCATCTGTCATCGGAGAAT TTAAGGGTCTCTTCAAAATTTATCCCCTCCCAGAAGATCCAGCCATCCCCATGCCCCCGAGACAATTCCA TCAGCTGGCTGCTCAGGGTCCCCAGGAATGCTTGGTCCGTATTTATATCGTCCGAGCATTTGGCTTACAG CCCAAGGACCCCAATGGCAAGTGTGACCCTTACATCAAGATCTCCATAGGGAAGAAATCAGTGAGTGACC AGGATAACTACATCCCTTGCACCCTGGAGCCTGTATTCGGAAAGATGTTTGAACTCACCTGCACTCTACC TCTGGAGAAGGACCTGAAGATCACACTCTACGATTATGACCTGCTCTCCAAGGATGAGAAGATTGGGGAG ACGGTCATAGACCTGGAGAACAGGCTGCTGTCCAAGTTTGGGGCTCGCTGTGGGCTCCCACAGACCTACT GTGTCTCTGGACCAAATCAGTGGAGAGATCAGCTCCGCCCCTCCCAGCTCCTCCACCTCTTCTGCCAGCA GCACAGGATCAAGGCCCCCGTGTACCGGACAGACCGAGTGACGTTTCAGGATAAGGACTACACCATTGAG GAGATAGAGGCTGGCAGACTCCCAAACCCACACCTGGGCCCAGTGGAGGAACGCTTAGCCCTGCATGTCC TTCAGCAACAAGGCTTGGTTCCTGAGCATGTGGAGTCACGGCCTCTTTATAGTCCTCTGCAGCCAGATAT CGAGCAGGGGAAGCTACAGATGTGGATTGACATATTTCCAAAGGTGCTGGGCCGGCCTGGACCTCCCTTC AACATCACCCCACGGAAAGCTAGAAGGTTTTTTCTGCGTTGTATTATTTGGAACACAAAAGATGTGATCT TGGATGACCTCAGTCTCACGGGGGAGAAGATGAGTGACATCTATGTGAAAGGCTGGATGGTGGGATTCGA AGAACACAAGCAGAAGACAGATGTGCACTACCGCTCCCTGGGTGGGGAGGGCAATTTCAACTGGAGGTTC GTGTTTCCCTTCGACTATCTGCCTGCTGAGCAAGTCTGTGCTGTTGCCAAGAAGGATGCCTTCTGGAGAC TCGACAAGACAGAGAGCAAGATCCCAGCACGTGTGGTCTTCCAGATCTGGGACAATGACAAATTCTCTTT TGATGACTTTCTGGGCTCTCTGCAGCTGGATCTCAACCGAATGCCCAAGCCAGCCAAGACGGCTGAGAAG TGCTCCTTGGACCAGCTGGATGACACCTTCCACCCAGAATGGTTTGTGTCCCTTTTTGAGCAGAAGACAG TGAAAGGATGGTGGCCTTGTGTGACAGAGGAGGGCGAGAAGAAGATGTTGGCGGGCAAGCTGGAAATGAC CTTGGAGATTGTTGCAGAAAGTGAACATGAAGAGCGGCCTGCTGGCCAAGGTCGGGATGAACCCAACATG AATCCGAAGCTAGAGGATCCAAGGCGCCCCGATACTTCTTTCCTGTGGTTCACCTCCCCGTACAAGACCA TGAAGTTCATCTTGTGGCGACGCTTCCGGTGTGCCATCATCCTCTTCATCATCCTCTTCATCCTCCTGCT CTTCCTGGGTGTCTTTGTCTACGCCTTCCCGAACTACGCTGCCATGAAGCTGGTGAAGCCCTTTCGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225180 representing NM_001077694
Red=Cloning site Green=Tags(s) MLRVFILFAENVHTPDSDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELLVVVK DHETMGRNRFLGEAKIPLQEVLATPSLSASFNAPLLDAKQQPTGASLVLQVSYTPPPGAVPLFPPPASLA PSPTLPDMDLVPGGGQSRAETWSLLSDSTMDTRYSGKKWPVPTDTGGEEDTEDQGLTGDEAEPFLDQSAA VGPGGPTTPRKPPSHPPPHYPGAKRKRSSAPPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAG QTKRTRIQKGNSPLFNETLFFNVFDSPLELFDEPIFITVVDSRSLRTDALLGEFRMDVGTVYREPRHAYL RKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLDKKDPSEDKEDIEGNLLRPTGVALRGAHFCLKLFR AEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCE KMRIRVMDWDRLTHNDTVATTYLGMSKISATGGEIEVDDNLGFLPTFGPCYVNLYGSPREFTGFPDPYAE LNTGKGEGVAYRGRVLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAI QFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIEIQNQLLRV ADRLEANLEQVHLALKAQCSSEDVDALVAQLTDELLADCSQPLCDIHEIPSATHLDQYLLRLRTRHLSQI KEAALALKLGHSELSTALEQAEDWLLHLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHEVLFSRRGP SYCGRNCGKLQTIFLKYPMEGMPGARMPVQIRIKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALV GNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDADAGHLSFVEEVFENQTRLP GGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVE KMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPL EKTGPAAVFALEGALGGMVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYLYQARDLPAMDKDS FSDPYAIVSFLHQSQKTVVEKNTLNPTWDQTLIFYEIEIFGEPASIAEHPPCIVVELYDHDTYGADEFMG RCICQPSLERMPRLAWFPLTRGSQPAGELLAAFELIQREKPAIHHIPGFEMHETSRILDETEDTDLPYPP PQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQMASISSPSLVVECGGQTVQSCVIRNLRKNPNFD VCTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLENFLCDPYSAESPSPQGGPDDVSLLS PGEDVLIDIDDKEPLIPVQEEEFIDWWSKFFASVGEREKCGSYLEKDFDTLKVYDTQLENVEAFGGLSDF CNTFKLYRGRTQEETDDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQ PKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGE TVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRIKAPVYRTDRVTFQDKDYTIE EIEAGRLPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDIFPKVLGRPGPPF NITPRKARRFFLRCIIWNTKDVILDDLSLTGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRF VFPFDYLPAEQVCAVAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEK CSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVTEEGEKKMLAGKLEMTLEIVAESEHEERPAGQGRDEPNM NPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGVFVYAFPNYAAMKLVKPFR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001077694 |
| ORF Size | 6297 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001077694.2, NP_001071162.1 |
| RefSeq Size | 6836 bp |
| RefSeq ORF | 6300 bp |
| Locus ID | 26903 |
| UniProt ID | Q9ESD7 |
| Cytogenetics | 6 36.14 cM |
| Gene Summary | Key calcium ion sensor involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress.[UniProtKB/Swiss-Prot Function] |
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