Dysf (NM_001077694) Mouse Untagged Clone

CAT#: MC225169

Dysf (untagged) - Mouse dysferlin (Dysf), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dysf
• Synonyms: 2310004N10Rik; AI604795; D6Pas3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225169 representing NM_001077694
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCGAGTCTTCATCCTTTTTGCGGAGAATGTCCACACCCCGGACTCCGACATCAGCGATGCCTACT
  GCTCCGCGGTGTTTGCAGGGGTAAAGAAGAGAACCAAAGTCATCAAGAACAGTGTGAACCCCGTGTGGAA
  TGAGGGCTTTGAGTGGGACCTCAAAGGTATTCCTCTAGATCAGAGCTCAGAACTTCTCGTGGTGGTCAAG
  GACCATGAGACGATGGGAAGAAACAGGTTCCTGGGGGAAGCCAAGATCCCACTCCAGGAGGTCCTTGCCA
  CCCCCAGCCTCTCTGCCAGCTTCAACGCACCTCTGCTAGACGCCAAGCAGCAACCCACGGGGGCCTCTCT
  GGTCCTGCAGGTGTCCTACACGCCACCCCCAGGAGCTGTGCCCCTGTTCCCACCACCTGCTTCTCTAGCA
  CCCTCCCCGACACTGCCTGACATGGATCTGGTGCCTGGTGGGGGACAGAGCCGGGCTGAGACTTGGTCCC
  TGCTCAGTGACAGCACCATGGACACGAGATACTCTGGGAAGAAGTGGCCGGTCCCCACGGACACAGGAGG
  GGAGGAAGACACCGAGGACCAGGGCCTGACTGGAGATGAAGCTGAGCCGTTCCTGGACCAGAGTGCTGCT
  GTGGGCCCAGGGGGACCGACCACACCCAGGAAGCCACCTTCCCACCCTCCTCCCCACTATCCAGGGGCAA
  AAAGAAAAAGAAGCTCTGCCCCACCTAGAAAGCTGCTGTCAGACAAGCCACAGGACTTCCAGATCAGAGT
  CCAGGTGATAGAGGGGCGTCAGCTGCCTGGGGTGAATATTAAGCCTGTGGTCAAGGTCACAGCTGCTGGG
  CAGACCAAGCGAACTCGGATCCAGAAGGGAAATAGCCCACTCTTCAATGAGACTCTTTTCTTCAACGTAT
  TTGACTCTCCCTTGGAGCTGTTTGATGAGCCCATCTTCATCACGGTGGTGGACTCACGCTCCCTTAGGAC
  AGATGCCCTCCTTGGCGAGTTCCGGATGGATGTGGGCACCGTGTACAGAGAGCCCCGACACGCTTATCTC
  CGGAAGTGGCTGCTGCTCTCCGACCCTGATGACTTCTCTGCGGGGGCCAGAGGCTACCTCAAAGCCAGCC
  TGTGTGTGCTAGGGCCTGGAGATGAAGCTCCCCTGGACAAGAAGGACCCTTCTGAAGACAAAGAGGACAT
  TGAAGGCAACCTGCTTAGGCCCACCGGTGTGGCCCTTCGAGGAGCCCACTTCTGCCTGAAGCTCTTCCGG
  GCTGAGGACTTACCACAGATGGACGATGCTGTGATGGACAACGTCAAGCAGATCTTTGGCTTTGACAGCA
  ACAAGAAGAACTTGGTGGATCCCTTTGTGGAAGTCAGCTTTGCTGGGAAAATGCTCTGCAGCAAGATCCT
  GGAGAAGACAGCTAACCCTCAGTGGAACCAGAACATCACCTTGCCTGCGATGTTTCCCTCTATGTGTGAA
  AAAATGAGGATTCGTGTCATGGACTGGGACCGCCTCACTCACAATGACACTGTGGCCACCACCTACTTGG
  GTATGTCGAAAATCTCTGCTACTGGAGGAGAAATAGAAGTGGACGACAACCTGGGCTTCCTCCCCACTTT
  TGGGCCCTGCTATGTGAACCTCTATGGCAGTCCCCGAGAGTTCACGGGCTTCCCAGATCCTTATGCAGAG
  CTCAACACAGGAAAGGGAGAAGGTGTGGCTTATCGAGGCAGGGTTCTGTTGTCCCTGGAGACCAAACTGG
  TGGAACACAGTGAGCAGAAGGTGGAAGACCTTCCTGCTGATGATATCCTGCGTGTGGAGAAGTACCTGCG
  GAGGCGCAAATATTCCCTCTTTGCTGCCTTCTACTCAGCCACAATGCTGCAGGATGTGGATGACGCCATC
  CAGTTCGAGGTCAGCATTGGGAACTACGGGAACAAGTTCGACACCACCTGCCTGCCCTTGGCCTCCACCA
  CCCAGTACAGCCGGGCGGTCTTTGATGGATGCCACTACTATTACTTGCCTTGGGGCAACGTGAAGCCCGT
  GGTGGTGCTGTCCTCATACTGGGAAGACATCAGCCATCGAATTGAGATCCAAAACCAGCTCCTCAGGGTC
  GCTGACCGCCTGGAAGCTAACCTGGAGCAGGTCCACTTGGCTCTGAAGGCACAGTGTTCCTCCGAGGATG
  TGGACGCCCTGGTGGCTCAGTTGACAGATGAGCTCCTTGCAGACTGCAGCCAGCCCCTGTGTGACATCCA
  CGAGATACCTTCTGCCACCCACCTTGATCAGTACCTGCTCCGGCTACGCACCCGTCACCTGAGCCAGATC
  AAAGAAGCTGCTCTGGCCTTGAAGCTTGGCCATAGTGAGCTCTCCACTGCCCTGGAGCAGGCCGAGGACT
  GGCTTCTGCATCTCCGTGCCCTGGCCGAGGAGCCCCAGAACAGCCTGCCGGACATCATCATCTGGATGCT
  GCAGGGTGACAAGCGTGTGGCCTACCAGCGGGTGCCTGCCCATGAAGTCCTCTTCTCCCGGCGGGGCCCC
  AGCTACTGCGGCAGGAACTGTGGGAAGCTGCAGACCATCTTTCTGAAATATCCTATGGAAGGTATGCCTG
  GGGCCCGAATGCCCGTGCAGATTCGGATCAAGCTCTGGTTCGGGCTCTCTGTGGATGAGAAGGAATTCAA
  TCAGTTTGCTGAGGGAAAGCTCTCTGTCTTTGCCGAAACGTATGAGAACCAGACAAAGCTGGCACTGGTC
  GGGAACTGGGGCACAACGGGCCTCACCTACCCCAAGTTTTCTGATGTCACAGGCAAGATCAAACTACCAA
  AGGATAGCTTCCGCCCCTCAGCTGGCTGGGCCTGGGCAGGAGATTGGTTCGTGTGTCCAGAGAAGACCCT
  ACTCCATGATGCTGACGCTGGTCATCTGAGCTTCGTGGAGGAGGTGTTCGAGAACCAGACCCGGCTCCCT
  GGGGGCCAGTGGATTTACATGAGTGATAACTACACAGATGTGAATGGGGAGAAGGTACTTCCGAAGGATG
  ATATTGAATGCCCGCTGGGCTGGAAGTGGGAAGATGAAGAATGGTCCACAGACCTCAATCGGGCTGTTGA
  TGAACAAGGCTGGGAGTACAGCATCACCATACCCCCAGACCGAAAGCCAAAACACTGGGTCCCTGTTGAG
  AAGATGTATTACACTCACAGGCGTCGACGCTGGGTCCGACTGCGCAGGAGGGACCTCAGCCAGATGGAAG
  CACTGAAGAGGCACAGGCAGGCAGAGGCCGAGGGCGAGGGCTGGGAGTATGCCTCTCTCTTCGGCTGGAA
  GTTCCACCTGGAGTACCGCAAGACAGATGCCTTCCGCCGCCGCCGCTGGCGCCGCCGCATGGAGCCGCTG
  GAGAAGACGGGACCTGCAGCTGTGTTTGCCCTCGAGGGGGCCCTGGGCGGCATGGTGGATGACAAGAGCG
  AAGACTCCATGTCTGTTTCTACCCTGAGCTTTGGCGTTAACAGACCCACAATCTCCTGCATCTTTGACTA
  TGGGAACCGCTACCATCTGCGCTGCTACCTGTACCAGGCCCGAGACCTGCCAGCCATGGACAAGGACTCC
  TTCTCTGATCCCTACGCCATCGTCTCCTTCCTGCATCAAAGCCAGAAGACAGTGGTGGAGAAGAACACCC
  TGAATCCCACCTGGGATCAAACCCTCATCTTCTATGAGATTGAGATCTTCGGCGAGCCAGCCAGTATCGC
  CGAGCATCCGCCCTGCATCGTGGTGGAGCTCTATGACCACGACACCTATGGTGCTGACGAGTTCATGGGT
  CGCTGTATTTGTCAGCCAAGCCTGGAACGGATGCCCCGCCTGGCCTGGTTCCCACTGACCCGGGGCAGCC
  AGCCTGCCGGCGAGCTGCTGGCAGCGTTCGAGCTTATCCAGAGAGAGAAGCCAGCCATCCATCACATCCC
  TGGCTTTGAGATGCATGAAACTTCAAGGATTTTGGATGAGACGGAGGACACAGATTTGCCTTACCCACCA
  CCTCAAAGGGAGGCCAACATCTACATGGTTCCCCAGAACATTAAGCCAGCTCTCCAACGTACTGCCATTG
  AGATCCTGGCCTGGGGCCTGCGGAACATGAAGAGCTATCAGATGGCCAGCATCTCTTCCCCCAGCCTCGT
  GGTGGAGTGTGGTGGGCAGACAGTGCAGTCCTGTGTCATCAGAAACCTTCGGAAGAACCCCAACTTTGAT
  GTCTGTACTCTCTTCATGGAAGTGATGTTACCCAGAGAGGACCTCTACTGTCCACCTATTGTGGTCAAGG
  TCATTGATAATCGCCAGTTTGGCCGCCGGCCAGTTGTGGGTCAGTGTACCATTCGCTCCCTGGAGAACTT
  CCTGTGCGACCCATACTCAGCAGAGAGTCCATCCCCACAGGGTGGCCCAGATGATGTGAGCTTACTCAGC
  CCCGGGGAAGATGTGCTGATTGACATCGATGACAAGGAGCCTCTCATTCCTGTCCAGGAGGAGGAGTTCA
  TTGACTGGTGGAGCAAATTCTTTGCTTCTGTCGGGGAGAGGGAAAAGTGTGGTTCCTACCTGGAGAAGGA
  TTTTGACACCTTGAAGGTCTATGACACACAGCTGGAGAATGTGGAGGCTTTTGGAGGCCTGTCCGACTTT
  TGTAACACCTTCAAGCTCTACCGGGGCAGGACTCAGGAGGAGACAGATGATCCATCTGTCATCGGAGAAT
  TTAAGGGTCTCTTCAAAATTTATCCCCTCCCAGAAGATCCAGCCATCCCCATGCCCCCGAGACAATTCCA
  TCAGCTGGCTGCTCAGGGTCCCCAGGAATGCTTGGTCCGTATTTATATCGTCCGAGCATTTGGCTTACAG
  CCCAAGGACCCCAATGGCAAGTGTGACCCTTACATCAAGATCTCCATAGGGAAGAAATCAGTGAGTGACC
  AGGATAACTACATCCCTTGCACCCTGGAGCCTGTATTCGGAAAGATGTTTGAACTCACCTGCACTCTACC
  TCTGGAGAAGGACCTGAAGATCACACTCTACGATTATGACCTGCTCTCCAAGGATGAGAAGATTGGGGAG
  ACGGTCATAGACCTGGAGAACAGGCTGCTGTCCAAGTTTGGGGCTCGCTGTGGGCTCCCACAGACCTACT
  GTGTCTCTGGACCAAATCAGTGGAGAGATCAGCTCCGCCCCTCCCAGCTCCTCCACCTCTTCTGCCAGCA
  GCACAGGATCAAGGCCCCCGTGTACCGGACAGACCGAGTGACGTTTCAGGATAAGGACTACACCATTGAG
  GAGATAGAGGCTGGCAGACTCCCAAACCCACACCTGGGCCCAGTGGAGGAACGCTTAGCCCTGCATGTCC
  TTCAGCAACAAGGCTTGGTTCCTGAGCATGTGGAGTCACGGCCTCTTTATAGTCCTCTGCAGCCAGATAT
  CGAGCAGGGGAAGCTACAGATGTGGATTGACATATTTCCAAAGGTGCTGGGCCGGCCTGGACCTCCCTTC
  AACATCACCCCACGGAAAGCTAGAAGGTTTTTTCTGCGTTGTATTATTTGGAACACAAAAGATGTGATCT
  TGGATGACCTCAGTCTCACGGGGGAGAAGATGAGTGACATCTATGTGAAAGGCTGGATGGTGGGATTCGA
  AGAACACAAGCAGAAGACAGATGTGCACTACCGCTCCCTGGGTGGGGAGGGCAATTTCAACTGGAGGTTC
  GTGTTTCCCTTCGACTATCTGCCTGCTGAGCAAGTCTGTGCTGTTGCCAAGAAGGATGCCTTCTGGAGAC
  TCGACAAGACAGAGAGCAAGATCCCAGCACGTGTGGTCTTCCAGATCTGGGACAATGACAAATTCTCTTT
  TGATGACTTTCTGGGCTCTCTGCAGCTGGATCTCAACCGAATGCCCAAGCCAGCCAAGACGGCTGAGAAG
  TGCTCCTTGGACCAGCTGGATGACACCTTCCACCCAGAATGGTTTGTGTCCCTTTTTGAGCAGAAGACAG
  TGAAAGGATGGTGGCCTTGTGTGACAGAGGAGGGCGAGAAGAAGATGTTGGCGGGCAAGCTGGAAATGAC
  CTTGGAGATTGTTGCAGAAAGTGAACATGAAGAGCGGCCTGCTGGCCAAGGTCGGGATGAACCCAACATG
  AATCCGAAGCTAGAGGATCCAAGGCGCCCCGATACTTCTTTCCTGTGGTTCACCTCCCCGTACAAGACCA
  TGAAGTTCATCTTGTGGCGACGCTTCCGGTGTGCCATCATCCTCTTCATCATCCTCTTCATCCTCCTGCT
  CTTCCTGGGTGTCTTTGTCTACGCCTTCCCGAACTACGCTGCCATGAAGCTGGTGAAGCCCTTTCGATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001077694
• Insert Size: 6300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001077694.2, NP_001071162.1
• RefSeq Size: 6845 bp
• RefSeq ORF: 6300 bp
• Locus ID: 26903
• UniProt ID: Q9ESD7
• Cytogenetics: 6 36.14 cM
• Gene Summary: Key calcium ion sensor involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) contains an alternate 5' terminal exon and differs in the 5' UTR and the 5' coding region, compared to variant 1. It encodes isoform 2, which has a shorter and distinct N-terminus, compared to isoform 1.