Glis1 (NM_147221) Mouse Tagged ORF Clone

CAT#: MG224595

Glis1 (tGFP-tagged) - Mouse GLIS family zinc finger 1 (Glis1), (10ug)

Product Images

Circular map for MG224595

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Glis1
• Synonyms: Gli5; Gli6; GliH1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224595 representing NM_147221
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATTGCGAGGTGGCCGAGGCACTTTCGGACAAGAGGCCAAAGGAGGCCCCTGGTGCTCCTGGCCAGG
  GCCGCGGGCCTGTCAGCCTGGGAGCGCACATGGCCTTCAGGATTGCTGTGAGTGGTGGCGGCTGCGGGGA
  CGGGAACCCGCTAGACCTGCTGCCTCGGCTACCGGTGCCACCACCACGTGCCCACGATCTCCTTCGGCCC
  CGGAGCCCTCGAGACTATGGTGTGTCCAAGACCGGCAGCGGGAAGGTGAACGGGAGCTACGGGCACAGCT
  CAGAGAAGAGCCTGCTGGACCTGGACCTGGCCGAGGGTCCCAGCCCCTCCTGCCACCAGGGTCTGTTTCT
  TCCTGCAGGGACCCCACCACCCCGGGGTCACCCCCCTGTCTGTGAGAAGCTGCTGCACTTCCCCCACCCA
  AACAGGTCACCCAGACCTCAGGCTACGTTTGTGAACGGCAGCCTCCCAGCCGCTCAGCACATCAAGCAAG
  AAGCCCTACCGGACTACCAGGCCATGGTCAGCGCCCACACACCCCTGCCCACCCACTGCCGAGCCCCATC
  GTCCATGGGTCTGCCCTCAGACCTGGACTTTCCAGACCGAGGCCTCACCAACCCTGCACCTTCCTGCTAC
  CTTCTGGGCAATGAACCCATCTCAGACCTGGGTCCCCAACCCGAGGCCCACCTCCCCGAGGGCAGCCTGA
  AACGCTGCTGCCTCCTGGGCCTGCCCCCCACCTCTTCAGCCTCCTCCTCACCCTGTGCCTCCTCAGATAT
  CAATCCTGTCATCCACTCCTCCCAGACAGCTCTAGTTAGCTGTGTAAATGGACTCCGAAGCCCACCTCTG
  CCGGGAGACCTGGGGGGCCCTCCCAAGCGGTCACGGCCCGGGCCTGCATCCAGTGACGGCCAGGAGGGCA
  GCTTGCAGCTTGAAGCATGCCGGAAGTCAGGCTTCCTGAAGCAGGAGCCCATGGACGAGTTTTCAGAGCT
  TTTTGCTCCACACCACCAGGGTTTGCCACCCCCTTACCCCTTGCCTCAGTTGCCAACTGGCCCCGGCCTC
  GGAGGCCTAGGGCTGGGCCTGGCAGGTAGGATGGTTGCCGGTCGGCAGGCATGCCGCTGGGTGGACTGCT
  GCGCAGCCTACGAGCAGCAGGAGGAGCTGGTGCGGCACATCGAGAAGAGCCACATCGACCAGCGCAAGGG
  CGAAGACTTCACCTGCTTCTGGGCCGGGTGTGTGCGGCGCTACAAGCCCTTCAATGCCCGCTACAAGCTG
  CTCATCCACATGAGGGTACACTCAGGCGAGAAGCCCAACAAGTGCATGTTCGAAGGCTGCAGTAAAGCCT
  TTTCCCGTCTGGAGAACCTGAAGATCCATCTGCGGAGCCACACAGGCGAGAAACCATACCTGTGCCAGCA
  CCCAGGCTGCCAGAAGGCCTTCAGCAACTCCAGCGACCGTGCCAAGCACCAACGCACCCACCTCGACACG
  AAGCCATATGCTTGTCAGATCCCTGGCTGCTCCAAGCGCTACACGGACCCCAGCTCCCTCCGCAAGCACG
  TGAAGGCCCACTCAGCCAAAGAGCAGCAGGTGCGTAAGAAGCTGCACACAGGTGCCGACCCAGAGGCTGA
  TGTTCTGTCCGAGTGTCTGTCCCTGCAGCAGCTCCAAGCATCCACACTGTTGCCGGCCAGCAGAGGGAAG
  GGCAGCCAAACCCTGAGCCAGGAGCTCCTCCCAGGTGTGTATCCTGGCTCCGTCACCCCACAAAACGGGC
  TTGCTTCAGGCATCCTGTCCCCCTCCCACGATGTCCCTTCCAGGCACCACCCACTGGAGGTCCCCACTGG
  TTCCCACCACCACCTGTCCCCTCTGCCCACAGCTGAGAGCACCAGGGATGGCCTGGGGCCCAGTCTCCTT
  TCACCCATGGTCAGCCCACTGAAGGGGCTTGGTCCCCCACCGCTACCACCAGCCTCCCAGAGTCAGTCTC
  CAGGGGGACAGTCATTCTCTACAGTCCCCAGCAAGCCTACCTACCCATCCTTCCAAAGCCCACCACCTCT
  GCCCAGCCCCCAAGGCTACCAAGGCAGTTTCCATTCCATCCAGAACTGCTTCCCCTACGCTGACTGCTAC
  CGGGCCACTGAGCCAGCAGCCTCCAGGGATGGACTGGTGGGTGATGCCCACGGTTTCAACCCCTTGCGAC
  CCAGCACATACTCCAGCCTCAGCACACCTTTATCCGCACCAGGCTACGAGACCCTGGCAGAAACGCCGTG
  TCCCCCAGCGCTGCAGCCACAGCCAGCTGAAGACCTGGTACCTAGTGGTCCTGAGGACTGTGGCTTCTTC
  CCCAATGGGGCCTTTGACCACTGTCTGAGTCACATCCCGTCCATCTACACTGACACC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224595 representing NM_147221
  Red=Cloning site Green=Tags(s)
  
  MHCEVAEALSDKRPKEAPGAPGQGRGPVSLGAHMAFRIAVSGGGCGDGNPLDLLPRLPVPPPRAHDLLRP
  RSPRDYGVSKTGSGKVNGSYGHSSEKSLLDLDLAEGPSPSCHQGLFLPAGTPPPRGHPPVCEKLLHFPHP
  NRSPRPQATFVNGSLPAAQHIKQEALPDYQAMVSAHTPLPTHCRAPSSMGLPSDLDFPDRGLTNPAPSCY
  LLGNEPISDLGPQPEAHLPEGSLKRCCLLGLPPTSSASSSPCASSDINPVIHSSQTALVSCVNGLRSPPL
  PGDLGGPPKRSRPGPASSDGQEGSLQLEACRKSGFLKQEPMDEFSELFAPHHQGLPPPYPLPQLPTGPGL
  GGLGLGLAGRMVAGRQACRWVDCCAAYEQQEELVRHIEKSHIDQRKGEDFTCFWAGCVRRYKPFNARYKL
  LIHMRVHSGEKPNKCMFEGCSKAFSRLENLKIHLRSHTGEKPYLCQHPGCQKAFSNSSDRAKHQRTHLDT
  KPYACQIPGCSKRYTDPSSLRKHVKAHSAKEQQVRKKLHTGADPEADVLSECLSLQQLQASTLLPASRGK
  GSQTLSQELLPGVYPGSVTPQNGLASGILSPSHDVPSRHHPLEVPTGSHHHLSPLPTAESTRDGLGPSLL
  SPMVSPLKGLGPPPLPPASQSQSPGGQSFSTVPSKPTYPSFQSPPPLPSPQGYQGSFHSIQNCFPYADCY
  RATEPAASRDGLVGDAHGFNPLRPSTYSSLSTPLSAPGYETLAETPCPPALQPQPAEDLVPSGPEDCGFF
  PNGAFDHCLSHIPSIYTDT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_147221
• ORF Size: 2367 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_147221.2, NP_671754.2
• RefSeq Size: 2904 bp
• RefSeq ORF: 2370 bp
• Locus ID: 230587
• UniProt ID: Q8K1M4
• Cytogenetics: 4 C7
• Gene Summary: Acts as both a repressor and activator of transcription (PubMed:12042312, PubMed:12385751, PubMed:21654807). Binds to the consensus sequence 5'-GACCACCCAC-3' (PubMed:12042312). By controlling the expression of genes involved in cell differentiation inhibits the lineage commitment of multipotent cells (PubMed:21654807, PubMed:30544251). Prevents, for instance, the differentiation of multipotent mesenchymal cells into adipocyte and osteoblast (PubMed:30544251).[UniProtKB/Swiss-Prot Function]