Adssl1 (NM_007421) Mouse Tagged ORF Clone

CAT#: MG224498

Adssl1 (tGFP-tagged) - Mouse adenylosuccinate synthetase like 1 (Adssl1), (10ug)

Product Images

Circular map for MG224498

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Adssl1
• Synonyms: Adss; Adss1; AI528595
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224498 representing NM_007421
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGGGACCCGAGCCTCCAACGACCGGCCCCCCGGCACAGGCGGAGTCAAGAGGGGGCGGCTGCAGC
  AGGAGGCGGCGGCGACAGGCTCCCGCGTGACCGTGGTGCTGGGCGCGCAGTGGGGGGACGAGGGCAAAGG
  CAAGGTGGTGGACCTGCTGGCCACGGACGCCGACATCGTCAGTCGCTGCCAGGGGGGCAACAATGCGGGG
  CACACAGTAGTGGTGGACGGCAAGGAGTATGACTTTCACCTGCTCCCCAGCGGCATCATCAACACCAAGG
  CCGTGTCATTCATTGGCAATGGCGTGGTCATCCACTTGCCGGGCTTGTTTGAAGAGGCAGAGAAGAACGA
  GAAGAAAGGGCTGAAGGACTGGGAGAAGCGTCTCATCATCTCTGACCGGGCTCACCTTGTGTTCGACTTC
  CACCAGGCAGTGGACGGGCTGCAGGAAGTACAACGTCAAGCACAAGAGGGGAAGAATATCGGCACCACCA
  AGAAGGGAATCGGACCAACTTACTCCTCCAAAGCTGCCCGGACAGGCCTCCGCATCTGTGACCTTCTCTC
  GGATTTTGACGAGTTTTCTGCCAGATTCAAGAACCTGGCCCACCAGCACCAGTCCATGTTCCCCACCCTG
  GAAATAGATGTGGAAGGTCAACTCAAAAGGCTCAAGGGCTTTGCTGAGCGCATCAGACCCATGGTTCGAG
  ATGGTGTCTACTTCATGTATGAAGCCCTGCATGGTCCCCCCAAAAAGGTCCTGGTGGAAGGTGCCAACGC
  GGCCCTGCTCGACATCGATTTCGGGACCTACCCCTTTGTGACTTCTTCCAACTGCACTGTGGGTGGTGTG
  TGCACCGGCCTGGGCATCCCGCCCCAGAACATAGGCGATGTGTATGGTGTGGTAAAGGCCTACACCACCC
  GTGTGGGCATTGGGGCCTTCCCCACGGAGCAGATCAATGAAATTGGAGATCTGCTGCAGAACCGTGGCCA
  TGAGTGGGGGGTGACCACAGGCAGGAAGAGGCGCTGCGGCTGGCTGGACCTGATGATCCTCCGATACGCT
  CACATGGTCAATGGCTTCACTGCGCTGGCATTGACCAAGCTGGACATACTGGATGTCCTGAGCGAGATTA
  AAGTTGGCATCTCCTACAAGCTCAACGGAAAGAGGATTCCCTACTTCCCTGCTAACCAGGAGATCCTGCA
  GAAGGTGGAGGTCGAGTATGAAACACTGCCTGGATGGAAAGCAGACACCACAGGCGCCAGGAAGTGGGAG
  GACCTGCCTCCGCAGGCCCAAAGCTACGTGCGCTTCGTGGAGAATCACATGGGTGTTGCAGTCAAATGGG
  TCGGCGTTGGGAAGTCCAGAGAGTCCATGATCCAGCTTTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224498 representing NM_007421
  Red=Cloning site Green=Tags(s)
  
  MSGTRASNDRPPGTGGVKRGRLQQEAAATGSRVTVVLGAQWGDEGKGKVVDLLATDADIVSRCQGGNNAG
  HTVVVDGKEYDFHLLPSGIINTKAVSFIGNGVVIHLPGLFEEAEKNEKKGLKDWEKRLIISDRAHLVFDF
  HQAVDGLQEVQRQAQEGKNIGTTKKGIGPTYSSKAARTGLRICDLLSDFDEFSARFKNLAHQHQSMFPTL
  EIDVEGQLKRLKGFAERIRPMVRDGVYFMYEALHGPPKKVLVEGANAALLDIDFGTYPFVTSSNCTVGGV
  CTGLGIPPQNIGDVYGVVKAYTTRVGIGAFPTEQINEIGDLLQNRGHEWGVTTGRKRRCGWLDLMILRYA
  HMVNGFTALALTKLDILDVLSEIKVGISYKLNGKRIPYFPANQEILQKVEVEYETLPGWKADTTGARKWE
  DLPPQAQSYVRFVENHMGVAVKWVGVGKSRESMIQLF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007421
• ORF Size: 1371 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007421.2, NP_031447.1
• RefSeq Size: 1806 bp
• RefSeq ORF: 1374 bp
• Locus ID: 11565
• UniProt ID: P28650
• Cytogenetics: 12 F1
• Gene Summary: Component of the purine nucleotide cycle (PNC), which interconverts IMP and AMP to regulate the nucleotide levels in various tissues, and which contributes to glycolysis and ammoniagenesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.[UniProtKB/Swiss-Prot Function]